Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HUK68_RS01310 Genome accession   NZ_CP054840
Coordinates   271293..272429 (-) Length   378 a.a.
NCBI ID   WP_175502567.1    Uniprot ID   A0A6N1WXD7
Organism   Comamonas antarctica strain 16-35-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 266293..277429
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUK68_RS01280 (HUK68_01280) - 266490..267389 (-) 900 WP_244146313.1 formylglycine-generating enzyme family protein -
  HUK68_RS01285 (HUK68_01285) rsmI 267564..268490 (-) 927 WP_175502562.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  HUK68_RS01290 (HUK68_01290) - 268490..268900 (+) 411 WP_175502563.1 YraN family protein -
  HUK68_RS01295 (HUK68_01295) - 268950..269558 (+) 609 WP_175502564.1 SIS domain-containing protein -
  HUK68_RS01300 (HUK68_01300) - 269555..270217 (+) 663 WP_175502565.1 BON domain-containing protein -
  HUK68_RS01305 (HUK68_01305) - 270346..271242 (-) 897 WP_175502566.1 NAD(P)-dependent oxidoreductase -
  HUK68_RS01310 (HUK68_01310) pilU 271293..272429 (-) 1137 WP_175502567.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HUK68_RS01315 (HUK68_01315) - 272472..273110 (-) 639 WP_175502568.1 cyclic nucleotide-binding domain-containing protein -
  HUK68_RS01320 (HUK68_01320) pilT 273187..274230 (-) 1044 WP_175502569.1 type IV pilus twitching motility protein PilT Machinery gene
  HUK68_RS01325 (HUK68_01325) - 274269..274997 (+) 729 WP_175502570.1 YggS family pyridoxal phosphate-dependent enzyme -
  HUK68_RS01330 (HUK68_01330) - 275133..276218 (-) 1086 WP_175502571.1 glycosyl hydrolase family 79 C-terminal domain-containing protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42307.75 Da        Isoelectric Point: 6.7010

>NTDB_id=454834 HUK68_RS01310 WP_175502567.1 271293..272429(-) (pilU) [Comamonas antarctica strain 16-35-5]
MERDQASKFINDLLQLMVSRNGSDLFITAEFPPAIKVDGKITKVSPQPLTALHTLTLARSIMSDKQIADFERTKESNFAI
SPAGIGRFRVNTFMQQGRIGMVMRTIPMTVPTIDKLGVPQVLKEVSMSKRGLCILVGATGSGKSTTLAAMVDWRNENSYG
HIITVEDPIEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAIAFSETGHLCLATLHANNA
NQALDRIVNFFPEERRQQLLMDLSLNLRAMVSQRLIPRQDAKGRVAAMEVMLNTPLISDLIFKGEVAEIKEVMKKGRDLG
MQTFDQSLYDLFEAQLITYEDALRNADSVNDLRLNIKLNSQRARNLDLSSGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=454834 HUK68_RS01310 WP_175502567.1 271293..272429(-) (pilU) [Comamonas antarctica strain 16-35-5]
ATGGAACGAGACCAGGCCAGTAAATTCATCAACGATCTGCTCCAGCTGATGGTCAGCCGCAATGGCAGCGACCTGTTCAT
CACCGCCGAATTCCCGCCCGCCATCAAGGTCGATGGCAAGATCACCAAGGTCTCGCCCCAGCCGCTCACGGCGCTGCACA
CGCTGACGCTGGCGCGCTCGATCATGAGCGACAAGCAGATCGCCGACTTCGAGCGCACCAAGGAAAGCAACTTCGCGATC
TCGCCCGCGGGCATCGGGCGCTTTCGCGTCAACACCTTCATGCAGCAGGGCCGCATCGGCATGGTCATGCGCACCATCCC
GATGACCGTGCCGACCATCGACAAGCTCGGCGTGCCGCAGGTGCTCAAGGAGGTCTCGATGAGCAAGCGCGGCCTGTGCA
TCCTGGTCGGCGCCACCGGCTCGGGCAAGTCGACCACGCTGGCCGCCATGGTCGACTGGCGCAACGAGAACTCCTACGGC
CACATCATCACCGTCGAGGACCCGATCGAGTTCGTGCATCCGCACAAGAACTGCGTGGTCACCCAGCGCGAGGTCGGCCT
CGACACCGACAGCTGGGAGGCGGCGCTGAAGAACACGCTGCGCCAGGCGCCCGATGTCATCCTGATGGGCGAAATCCGCG
ACCGCGAGACCATGGAGCATGCGATTGCGTTCTCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAACGCC
AACCAGGCGCTGGACCGCATCGTCAACTTCTTTCCCGAGGAGCGGCGCCAGCAGCTGCTGATGGACCTGTCGCTGAACCT
GCGCGCCATGGTCTCGCAGCGGCTGATCCCGCGCCAGGACGCCAAGGGCCGCGTCGCGGCCATGGAGGTCATGCTCAACA
CGCCGCTGATCTCCGACCTGATCTTCAAGGGCGAGGTCGCCGAGATCAAGGAAGTCATGAAGAAGGGCCGCGACCTGGGC
ATGCAGACCTTCGACCAGTCGCTGTACGATCTGTTCGAGGCCCAGCTCATCACCTACGAGGATGCGCTGCGCAATGCCGA
CTCGGTCAACGACCTGCGCCTGAACATCAAGCTCAACAGCCAGCGCGCACGCAACCTCGACCTGTCCTCGGGCACCGAAC
ACTTCGCGATTGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(36-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N1WXD7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.751

92.328

0.579
  pilU Acinetobacter baylyi ADP1

56.825

94.974

0.54
  pilU Vibrio cholerae strain A1552

51.791

96.032

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.994

89.153

0.41
  pilT Pseudomonas aeruginosa PAK

45.104

89.153

0.402
  pilT Pseudomonas stutzeri DSM 10701

43.027

89.153

0.384
  pilT Legionella pneumophila strain ERS1305867

43.243

88.095

0.381
  pilT Legionella pneumophila strain Lp02

43.243

88.095

0.381
  pilT Acinetobacter nosocomialis M2

43.243

88.095

0.381
  pilT Acinetobacter baumannii D1279779

43.243

88.095

0.381
  pilT Acinetobacter baumannii strain A118

43.243

88.095

0.381
  pilT Acinetobacter baylyi ADP1

42.643

88.095

0.376