Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HTY51_RS15310 Genome accession   NZ_CP054424
Coordinates   3174927..3175970 (+) Length   347 a.a.
NCBI ID   WP_174253530.1    Uniprot ID   A0A6N0BUZ7
Organism   Rhodoferax sp. BAB1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3169927..3180970
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HTY51_RS18805 (HTY51_15280) - 3170349..3170786 (-) 438 WP_305791370.1 prepilin-type cleavage/methylation domain-containing protein -
  HTY51_RS15285 (HTY51_15285) - 3170920..3171642 (-) 723 WP_174253525.1 TetR/AcrR family transcriptional regulator -
  HTY51_RS15290 (HTY51_15290) - 3171696..3172466 (+) 771 WP_174253526.1 alpha/beta fold hydrolase -
  HTY51_RS15295 (HTY51_15295) - 3172536..3173048 (+) 513 WP_174253527.1 flavin reductase family protein -
  HTY51_RS15300 (HTY51_15300) - 3173081..3174175 (+) 1095 WP_174253528.1 LLM class flavin-dependent oxidoreductase -
  HTY51_RS15305 (HTY51_15305) - 3174172..3174873 (-) 702 WP_174253529.1 YggS family pyridoxal phosphate-dependent enzyme -
  HTY51_RS15310 (HTY51_15310) pilT 3174927..3175970 (+) 1044 WP_174253530.1 type IV pilus twitching motility protein PilT Machinery gene
  HTY51_RS15315 (HTY51_15315) pilU 3176042..3177178 (+) 1137 WP_174253531.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HTY51_RS15320 (HTY51_15320) - 3177352..3178257 (+) 906 WP_174253532.1 NAD(P)-dependent oxidoreductase -
  HTY51_RS15325 (HTY51_15325) - 3178289..3178957 (-) 669 WP_371733841.1 BON domain-containing protein -
  HTY51_RS15330 (HTY51_15330) - 3178954..3179553 (-) 600 WP_174253533.1 phosphoheptose isomerase -
  HTY51_RS15335 (HTY51_15335) - 3179609..3180001 (-) 393 WP_174253534.1 YraN family protein -
  HTY51_RS15340 (HTY51_15340) rsmI 3180001..3180915 (+) 915 WP_174253535.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38129.89 Da        Isoelectric Point: 6.8888

>NTDB_id=452435 HTY51_RS15310 WP_174253530.1 3174927..3175970(+) (pilT) [Rhodoferax sp. BAB1]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVEALGHKEVHAMVYDIMNDTQRKNYEEFLEVDFSFEIDGLAR
FRVNAFNHNRGAGAVFRTIPSKILTLEQLNAPKIFGELALRPRGMVLVTGPTGSGKSTTLAAMVNYLNETEFGHILTVED
PIEFVHESKKCLINQREVGPHTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMIRAMLSESLQAVISQTLCKTKDGSGRVAAHEIMLGTPAIRNLIREAKVAQMYSSIQTGNSVGMQTLDQN
LTDLVKRNIISAAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=452435 HTY51_RS15310 WP_174253530.1 3174927..3175970(+) (pilT) [Rhodoferax sp. BAB1]
GTGGACATTACCCAACTGCTGGCATTCAGCGTCAAGAACAAGGCCTCCGACCTGCACCTCTCCGCCGGCCTGCCGCCCAT
GATCCGCGTGCACGGCGACGTGCGCCGCATCAACGTCGAGGCCCTGGGGCACAAGGAAGTGCACGCCATGGTGTACGACA
TCATGAACGACACCCAGCGCAAGAATTACGAAGAATTCCTGGAAGTCGACTTCTCCTTTGAGATCGATGGCCTGGCGCGC
TTCCGGGTCAATGCCTTCAACCACAACCGCGGCGCGGGCGCCGTGTTCCGGACCATCCCGAGCAAGATCCTGACCCTGGA
GCAGCTCAACGCCCCCAAGATCTTCGGTGAACTGGCGCTCAGGCCGCGCGGCATGGTGCTGGTGACCGGCCCCACGGGCT
CGGGCAAGTCCACCACCCTGGCGGCCATGGTCAACTACCTCAACGAGACCGAGTTCGGCCACATCCTGACGGTGGAAGAC
CCGATCGAGTTCGTGCACGAATCCAAGAAGTGCCTGATCAACCAGCGTGAAGTCGGCCCGCACACCCTGAGTTTCGCCGC
CGCCCTGAAATCGGCCCTGCGCGAAGACCCGGACGCCATCCTGGTGGGCGAAATGCGCGACCTGGAAACCATCCGCCTGG
CCATGACCGCAGCCGAGACCGGTCACCTGGTATTCGGCACCCTGCACACCTCCAGCGCCGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGAGGAAAAGGAAATGATCCGCGCCATGCTGTCCGAATCGCTGCAGGCCGTGATCTCGCAGAC
CCTGTGCAAGACCAAGGACGGCAGCGGCCGGGTGGCGGCGCACGAGATCATGCTGGGCACGCCGGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTATTCCAGCATCCAGACCGGCAACAGCGTGGGCATGCAGACGCTGGACCAGAAC
CTGACCGACTTGGTCAAGCGCAACATCATCAGCGCCGCGGAAGCCCGCGGCAAGGCCAAGATCCCCGAGAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N0BUZ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.812

99.424

0.764
  pilT Pseudomonas stutzeri DSM 10701

76.232

99.424

0.758
  pilT Acinetobacter nosocomialis M2

76.232

99.424

0.758
  pilT Acinetobacter baumannii D1279779

76.232

99.424

0.758
  pilT Acinetobacter baumannii strain A118

76.232

99.424

0.758
  pilT Acinetobacter baylyi ADP1

75.362

99.424

0.749
  pilT Legionella pneumophila strain Lp02

73.451

97.695

0.718
  pilT Legionella pneumophila strain ERS1305867

73.451

97.695

0.718
  pilT Neisseria gonorrhoeae MS11

68.696

99.424

0.683
  pilT Neisseria meningitidis 8013

68.696

99.424

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.449

98.271

0.663
  pilT Vibrio cholerae strain A1552

67.449

98.271

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Vibrio cholerae strain A1552

44.179

96.542

0.427
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.542

0.401