Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HTY51_RS15315 Genome accession   NZ_CP054424
Coordinates   3176042..3177178 (+) Length   378 a.a.
NCBI ID   WP_174253531.1    Uniprot ID   A0A6N0BTH0
Organism   Rhodoferax sp. BAB1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3171042..3182178
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HTY51_RS15290 (HTY51_15290) - 3171696..3172466 (+) 771 WP_174253526.1 alpha/beta fold hydrolase -
  HTY51_RS15295 (HTY51_15295) - 3172536..3173048 (+) 513 WP_174253527.1 flavin reductase family protein -
  HTY51_RS15300 (HTY51_15300) - 3173081..3174175 (+) 1095 WP_174253528.1 LLM class flavin-dependent oxidoreductase -
  HTY51_RS15305 (HTY51_15305) - 3174172..3174873 (-) 702 WP_174253529.1 YggS family pyridoxal phosphate-dependent enzyme -
  HTY51_RS15310 (HTY51_15310) pilT 3174927..3175970 (+) 1044 WP_174253530.1 type IV pilus twitching motility protein PilT Machinery gene
  HTY51_RS15315 (HTY51_15315) pilU 3176042..3177178 (+) 1137 WP_174253531.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HTY51_RS15320 (HTY51_15320) - 3177352..3178257 (+) 906 WP_174253532.1 NAD(P)-dependent oxidoreductase -
  HTY51_RS15325 (HTY51_15325) - 3178289..3178957 (-) 669 WP_371733841.1 BON domain-containing protein -
  HTY51_RS15330 (HTY51_15330) - 3178954..3179553 (-) 600 WP_174253533.1 phosphoheptose isomerase -
  HTY51_RS15335 (HTY51_15335) - 3179609..3180001 (-) 393 WP_174253534.1 YraN family protein -
  HTY51_RS15340 (HTY51_15340) rsmI 3180001..3180915 (+) 915 WP_174253535.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  HTY51_RS15345 (HTY51_15345) - 3180912..3181400 (+) 489 WP_174253536.1 low molecular weight protein-tyrosine-phosphatase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42003.32 Da        Isoelectric Point: 6.9078

>NTDB_id=452436 HTY51_RS15315 WP_174253531.1 3176042..3177178(+) (pilU) [Rhodoferax sp. BAB1]
MERDQATKFINDLLRLMISRNGSDLFITADFPPAIKVDGKVTKVSPQPLSSAHTMALARSIMNDKQIAEFERTKECNFAI
SPPNVGRFRANAFIQQGKVGLVLRVIPATLPTIDTLGVPQVLKEVTMAKRGLCIMVGATGSGKSTTLAAMVDWRNENSHG
HIITIEDPVEFVHPHKNCVVTQREVGLDTDSWEIALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIINFFPEERRPQLLMDLSLNLRALVSQRLVPKQDGKGRSAAVEVMLNSPLISDLIFKGEISEIKEIMKKSRNMG
MQTFDQALFDLYEGNQITYEDALRNADSVNDLRLQIKLNSQRGKTQDLSAGTENFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=452436 HTY51_RS15315 WP_174253531.1 3176042..3177178(+) (pilU) [Rhodoferax sp. BAB1]
ATGGAGCGCGACCAGGCAACCAAGTTCATCAACGACCTGCTGCGGCTGATGATCAGCCGCAACGGCAGCGATCTGTTCAT
CACCGCCGACTTCCCGCCCGCCATCAAGGTCGACGGCAAGGTGACCAAGGTGTCGCCCCAGCCCCTGAGCTCGGCCCACA
CCATGGCGCTGGCGCGTTCGATCATGAACGACAAGCAGATCGCCGAGTTCGAACGCACCAAGGAGTGCAACTTCGCGATC
TCGCCACCCAATGTCGGGCGCTTCCGTGCCAACGCCTTCATCCAGCAGGGCAAGGTCGGCCTGGTGCTGCGCGTGATTCC
CGCCACGCTGCCGACCATCGACACCCTGGGCGTGCCCCAGGTGCTCAAGGAAGTGACCATGGCCAAGCGCGGCCTGTGCA
TCATGGTGGGCGCCACCGGCTCGGGCAAGTCCACCACGCTGGCCGCCATGGTGGACTGGCGCAATGAGAACTCCCACGGC
CACATCATCACCATCGAGGACCCGGTCGAGTTCGTGCACCCGCACAAGAACTGCGTGGTGACACAGCGCGAAGTGGGGCT
GGACACCGACAGCTGGGAAATCGCCCTCAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTCATGGGTGAGATCCGCG
ACCGCGAGACCATGGAACACGCCGTGGCCTTTGCCGAAACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAGCGCC
AACCAGGCACTGGACCGCATCATCAACTTCTTCCCCGAAGAACGTCGTCCGCAACTGCTGATGGACCTGTCGCTCAACCT
GCGCGCCCTGGTGTCGCAGCGCCTCGTCCCCAAGCAGGACGGCAAGGGCCGCTCGGCGGCGGTGGAGGTCATGCTCAACT
CGCCGCTGATTTCCGACCTGATCTTCAAGGGCGAGATCTCCGAGATCAAGGAGATCATGAAAAAGAGCCGCAACATGGGC
ATGCAGACCTTCGACCAGGCCCTGTTCGACCTGTACGAAGGCAACCAGATCACCTACGAGGACGCGCTGCGCAACGCCGA
CTCGGTCAACGACCTGCGCCTGCAGATCAAGCTCAACAGCCAGCGAGGCAAGACACAGGACTTGAGCGCCGGCACCGAAA
ACTTCGCCATCGTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(45-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N0BTH0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.61

92.328

0.587
  pilU Acinetobacter baylyi ADP1

57.939

94.974

0.55
  pilU Vibrio cholerae strain A1552

53.444

96.032

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.401

89.153

0.405
  pilT Pseudomonas aeruginosa PAK

43.323

89.153

0.386
  pilT Legionella pneumophila strain Lp02

42.943

88.095

0.378
  pilT Legionella pneumophila strain ERS1305867

42.943

88.095

0.378
  pilT Pseudomonas stutzeri DSM 10701

42.136

89.153

0.376
  pilT Acinetobacter nosocomialis M2

43.887

84.392

0.37
  pilT Acinetobacter baumannii strain A118

43.887

84.392

0.37
  pilT Acinetobacter baumannii D1279779

43.887

84.392

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.218

83.862

0.362
  pilT Vibrio cholerae strain A1552

43.218

83.862

0.362