Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HPE48_RS19235 Genome accession   NZ_CP054368
Coordinates   3967759..3968739 (+) Length   326 a.a.
NCBI ID   WP_142436309.1    Uniprot ID   -
Organism   Escherichia coli strain SCU-115     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3962759..3973739
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPE48_RS19205 (HPE48_19190) yggM 3963364..3964371 (+) 1008 WP_000745230.1 DUF1202 family protein -
  HPE48_RS19210 (HPE48_19195) hemW 3964436..3965572 (-) 1137 WP_042342792.1 radical SAM family heme chaperone HemW -
  HPE48_RS19215 (HPE48_19200) rdgB 3965565..3966158 (-) 594 WP_001174738.1 XTP/dITP diphosphatase -
  HPE48_RS19220 (HPE48_19205) yggU 3966166..3966456 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  HPE48_RS19225 (HPE48_19210) yggT 3966453..3967019 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  HPE48_RS19230 (HPE48_19215) yggS 3967037..3967741 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  HPE48_RS19235 (HPE48_19220) pilT 3967759..3968739 (+) 981 WP_142436309.1 type IV pilus twitching motility protein PilT Machinery gene
  HPE48_RS19240 (HPE48_19225) ruvX 3968913..3969329 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  HPE48_RS19245 (HPE48_19230) yqgE 3969329..3969892 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  HPE48_RS19250 (HPE48_19235) gshB 3970001..3970951 (-) 951 WP_000593260.1 glutathione synthase -
  HPE48_RS19255 (HPE48_19240) rsmE 3970964..3971695 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HPE48_RS19260 (HPE48_19245) endA 3971775..3972482 (-) 708 WP_001305312.1 deoxyribonuclease I -
  HPE48_RS19265 (HPE48_19250) yggI 3972577..3973074 (-) 498 WP_032275985.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36073.30 Da        Isoelectric Point: 5.9975

>NTDB_id=452068 HPE48_RS19235 WP_142436309.1 3967759..3968739(+) (pilT) [Escherichia coli strain SCU-115]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRSAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VKEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=452068 HPE48_RS19235 WP_142436309.1 3967759..3968739(+) (pilT) [Escherichia coli strain SCU-115]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGAATGGAAGCTGCGCCGTTTGACGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCAGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCTGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGTTGTCACAAAAACTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGAAAGAAGGGCGCTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.483

100

0.466
  pilT Legionella pneumophila strain ERS1305867

45.566

100

0.457
  pilT Legionella pneumophila strain Lp02

45.566

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.453

97.546

0.414
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365
  pilB Legionella pneumophila strain ERS1305867

31.152

100

0.365