Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HQN60_RS08630 Genome accession   NZ_CP054143
Coordinates   1816835..1817878 (+) Length   347 a.a.
NCBI ID   WP_173533265.1    Uniprot ID   A0A6M8SPV5
Organism   Deefgea piscis strain D17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1811835..1822878
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HQN60_RS08595 (HQN60_08595) dsbD 1811990..1813540 (-) 1551 WP_173533258.1 protein-disulfide reductase DsbD -
  HQN60_RS08600 (HQN60_08600) cutA 1813541..1813798 (-) 258 WP_254456601.1 divalent-cation tolerance protein CutA -
  HQN60_RS08605 (HQN60_08605) - 1813875..1814288 (+) 414 WP_173533260.1 FxsA family protein -
  HQN60_RS08610 (HQN60_08610) - 1814290..1814583 (-) 294 WP_173533261.1 DUF167 domain-containing protein -
  HQN60_RS08615 (HQN60_08615) - 1814583..1815170 (-) 588 WP_173533262.1 YggT family protein -
  HQN60_RS08620 (HQN60_08620) proC 1815186..1816001 (-) 816 WP_173533263.1 pyrroline-5-carboxylate reductase -
  HQN60_RS08625 (HQN60_08625) - 1816051..1816746 (-) 696 WP_173533264.1 YggS family pyridoxal phosphate-dependent enzyme -
  HQN60_RS08630 (HQN60_08630) pilT 1816835..1817878 (+) 1044 WP_173533265.1 type IV pilus twitching motility protein PilT Machinery gene
  HQN60_RS08635 (HQN60_08635) pilU 1817891..1819027 (+) 1137 WP_173533266.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HQN60_RS08640 (HQN60_08640) - 1819474..1821447 (+) 1974 WP_173533267.1 FimV family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38379.15 Da        Isoelectric Point: 6.8274

>NTDB_id=451116 HQN60_RS08630 WP_173533265.1 1816835..1817878(+) (pilT) [Deefgea piscis strain D17]
MEISELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPAMEHKDVHDMVYDIMNDGQRKIYEDTLECDFSFEIPNLAR
FRVNAFVQNRGAGAVFRTIPSKVLSLEELGCPKVFQDIAQNPRGLVLVTGPTGSGKSTTLAAMVNFINDNEYGHILTVED
PIEFVHQSKKCLVNQREVGPHTMSFSNALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDGQGRVAAHEIMIGIPAIRNLIRENKIAQMYSSIQTGSQYGMQTLDQC
LQNLVQRNVVSIIDARGKAAIPDNFKG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=451116 HQN60_RS08630 WP_173533265.1 1816835..1817878(+) (pilT) [Deefgea piscis strain D17]
ATGGAAATCTCAGAACTCTTAGCCTTTTCGGTAAAGAATAAAGCATCCGATTTACACCTTTCTGCGGGTTTGCCACCGAT
GATTCGGGTTCATGGCGACGTTCGGCGCATTAATTTACCGGCAATGGAGCATAAAGACGTCCACGATATGGTGTACGACA
TTATGAATGATGGCCAGCGAAAAATTTACGAAGACACCCTAGAATGCGACTTTTCCTTTGAAATTCCCAATTTAGCGCGT
TTTCGGGTGAATGCCTTTGTGCAAAATCGCGGCGCTGGTGCGGTATTTCGGACGATTCCCTCTAAAGTGCTGTCATTAGA
AGAACTGGGCTGTCCCAAAGTCTTTCAGGACATTGCGCAAAATCCACGTGGTTTGGTGCTAGTCACCGGGCCAACTGGCT
CGGGCAAATCAACCACCCTCGCTGCCATGGTTAATTTTATTAATGACAATGAATATGGCCATATTCTGACGGTAGAAGAC
CCCATCGAGTTCGTACATCAATCTAAAAAATGCCTGGTTAATCAGCGTGAAGTCGGCCCGCACACAATGTCGTTTTCGAA
TGCATTGCGTAGCGCCTTACGTGAAGATCCGGATGTGATTTTGGTCGGTGAGATGCGCGATTTGGAAACCATTCGTTTGG
CACTCACCGCCGCAGAAACCGGGCATTTGGTGTTTGGCACCTTGCATACCACATCGGCCGCGAAAACCATTGACCGGATT
GTTGACGTTTTCCCTGCCGCAGAAAAAGAAATGGTTCGCTCTATGCTTTCTGAATCATTGCGCGCAGTGATTTCGCAAAC
CTTGTTAAAAACCAAAGATGGGCAAGGGCGCGTTGCGGCACATGAAATTATGATTGGGATTCCTGCGATTCGAAATTTAA
TCCGCGAAAATAAAATTGCGCAAATGTATTCATCGATTCAAACCGGCTCACAATATGGCATGCAAACCTTGGATCAGTGT
CTGCAAAATTTAGTGCAACGTAATGTGGTATCAATTATCGATGCGCGTGGTAAAGCGGCGATTCCAGATAATTTTAAGGG
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M8SPV5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

78.261

99.424

0.778
  pilT Acinetobacter baumannii strain A118

78.261

99.424

0.778
  pilT Acinetobacter nosocomialis M2

78.261

99.424

0.778
  pilT Pseudomonas stutzeri DSM 10701

76.812

99.424

0.764
  pilT Pseudomonas aeruginosa PAK

76.812

99.424

0.764
  pilT Acinetobacter baylyi ADP1

76.232

99.424

0.758
  pilT Neisseria meningitidis 8013

73.156

97.695

0.715
  pilT Legionella pneumophila strain Lp02

74.622

95.389

0.712
  pilT Legionella pneumophila strain ERS1305867

74.622

95.389

0.712
  pilT Neisseria gonorrhoeae MS11

72.861

97.695

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.751

97.406

0.66
  pilT Vibrio cholerae strain A1552

67.751

97.406

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

99.424

0.51
  pilU Vibrio cholerae strain A1552

42.687

96.542

0.412
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.542

0.406
  pilU Acinetobacter baylyi ADP1

39.481

100

0.395