Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HQN60_RS08635 Genome accession   NZ_CP054143
Coordinates   1817891..1819027 (+) Length   378 a.a.
NCBI ID   WP_173533266.1    Uniprot ID   A0A6M8SNE2
Organism   Deefgea piscis strain D17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1812891..1824027
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HQN60_RS08600 (HQN60_08600) cutA 1813541..1813798 (-) 258 WP_254456601.1 divalent-cation tolerance protein CutA -
  HQN60_RS08605 (HQN60_08605) - 1813875..1814288 (+) 414 WP_173533260.1 FxsA family protein -
  HQN60_RS08610 (HQN60_08610) - 1814290..1814583 (-) 294 WP_173533261.1 DUF167 domain-containing protein -
  HQN60_RS08615 (HQN60_08615) - 1814583..1815170 (-) 588 WP_173533262.1 YggT family protein -
  HQN60_RS08620 (HQN60_08620) proC 1815186..1816001 (-) 816 WP_173533263.1 pyrroline-5-carboxylate reductase -
  HQN60_RS08625 (HQN60_08625) - 1816051..1816746 (-) 696 WP_173533264.1 YggS family pyridoxal phosphate-dependent enzyme -
  HQN60_RS08630 (HQN60_08630) pilT 1816835..1817878 (+) 1044 WP_173533265.1 type IV pilus twitching motility protein PilT Machinery gene
  HQN60_RS08635 (HQN60_08635) pilU 1817891..1819027 (+) 1137 WP_173533266.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HQN60_RS08640 (HQN60_08640) - 1819474..1821447 (+) 1974 WP_173533267.1 FimV family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42539.72 Da        Isoelectric Point: 6.3749

>NTDB_id=451117 HQN60_RS08635 WP_173533266.1 1817891..1819027(+) (pilU) [Deefgea piscis strain D17]
MEKEQAAKFMHDLLRHMRSKNASDLFITVDFPPAMKIDGRVTPVSNQQLTAQHTKELARAIMNDRQAEDFEANKECNFAI
SPGSMGRFRVNAFMQQGRVGMVLRTINSEIPKLDELNLPPVLRDIAMTKRGLVIFVGGTGSGKSTSLAAMIGHRNENAYD
HIITIEDPIEYVHEHKNSIITQREVGVDTDSWMAALKNTLRQAPDVILIGEVRDRETMDYAIAFAETGHLCMATLHANSA
NQALDRIINFFPEERRSQLLMDLSLNLKAFVSQRLVPHLSGKGRVAAVEVMLNSPLISELIFKGEVHEIKEIMKKSRELG
MQTFDQSLFDLFEANRISYEDALRNADSVNDLRLQIKLNGTESKNRDVLSGLDHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=451117 HQN60_RS08635 WP_173533266.1 1817891..1819027(+) (pilU) [Deefgea piscis strain D17]
ATGGAAAAAGAACAAGCAGCCAAGTTTATGCATGATTTATTGCGGCATATGCGCAGTAAAAATGCTTCGGATTTATTTAT
TACCGTTGATTTTCCGCCAGCAATGAAAATCGACGGGCGAGTGACGCCGGTTTCAAATCAGCAACTGACCGCACAACACA
CCAAAGAATTAGCGCGGGCGATTATGAATGATCGCCAAGCTGAAGACTTTGAAGCCAATAAAGAATGTAATTTCGCGATT
AGCCCAGGATCAATGGGGCGCTTTCGGGTGAATGCGTTTATGCAGCAAGGCCGTGTTGGCATGGTGCTGCGAACGATTAA
TTCGGAAATTCCTAAACTCGATGAATTAAATTTGCCGCCAGTATTGCGTGATATTGCGATGACTAAGCGCGGTTTAGTGA
TTTTTGTTGGTGGGACTGGCTCAGGTAAATCCACATCGTTAGCGGCAATGATTGGTCATCGCAATGAAAATGCCTATGAC
CATATCATTACTATTGAAGATCCAATTGAATACGTGCACGAGCATAAAAATTCAATTATTACGCAGCGAGAAGTGGGCGT
TGATACTGATTCTTGGATGGCGGCGCTGAAAAATACGTTGCGCCAAGCGCCAGATGTGATTTTGATCGGCGAAGTGCGTG
ATCGTGAAACCATGGATTACGCGATTGCTTTTGCCGAAACCGGGCATCTTTGCATGGCAACCTTGCATGCCAACTCGGCC
AATCAAGCTCTGGATCGGATTATTAACTTTTTCCCAGAAGAGCGCCGTTCTCAGTTATTAATGGATTTATCGCTTAACTT
AAAAGCTTTTGTGTCACAACGCTTAGTACCGCACTTATCGGGTAAAGGCCGCGTTGCTGCCGTTGAAGTGATGCTCAATT
CGCCGTTGATTTCAGAGTTAATCTTTAAAGGTGAAGTGCACGAGATTAAAGAAATTATGAAAAAATCGCGTGAATTAGGC
ATGCAAACATTTGATCAGTCGCTATTTGATTTATTTGAAGCCAATCGCATTAGCTATGAAGATGCCTTACGTAATGCCGA
CTCGGTGAATGATTTACGCTTGCAAATCAAATTAAATGGCACAGAATCGAAAAATCGTGACGTTTTGTCTGGTCTAGATC
ATCTTGATATTGTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(35-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M8SNE2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.291

94.709

0.59
  pilU Acinetobacter baylyi ADP1

57.923

96.825

0.561
  pilU Vibrio cholerae strain A1552

54.31

92.063

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.269

88.624

0.41
  pilT Acinetobacter baylyi ADP1

43.027

89.153

0.384
  pilT Pseudomonas stutzeri DSM 10701

42.09

88.624

0.373
  pilT Acinetobacter nosocomialis M2

42.598

87.566

0.373
  pilT Legionella pneumophila strain ERS1305867

42.598

87.566

0.373
  pilT Acinetobacter baumannii strain A118

42.598

87.566

0.373
  pilT Acinetobacter baumannii D1279779

42.598

87.566

0.373
  pilT Legionella pneumophila strain Lp02

42.598

87.566

0.373
  pilT Pseudomonas aeruginosa PAK

41.791

88.624

0.37