Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I6H00_RS22945 Genome accession   NZ_CP065615
Coordinates   4559993..4560934 (+) Length   313 a.a.
NCBI ID   WP_197939774.1    Uniprot ID   -
Organism   Escherichia coli strain FDAARGOS_943     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4554993..4565934
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6H00_RS22915 (I6H00_22915) yggM 4555508..4556515 (+) 1008 WP_197939773.1 DUF1202 family protein -
  I6H00_RS22920 (I6H00_22920) hemW 4556670..4557806 (-) 1137 WP_000239917.1 radical SAM family heme chaperone HemW -
  I6H00_RS22925 (I6H00_22925) rdgB 4557799..4558392 (-) 594 WP_001174735.1 XTP/dITP diphosphatase -
  I6H00_RS22930 (I6H00_22930) yggU 4558400..4558690 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  I6H00_RS22935 (I6H00_22935) yggT 4558687..4559253 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  I6H00_RS22940 (I6H00_22940) yggS 4559271..4559975 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  I6H00_RS22945 (I6H00_22945) pilT 4559993..4560934 (+) 942 WP_197939774.1 type IV pilus twitching motility protein PilT Machinery gene
  I6H00_RS22950 (I6H00_22950) ruvX 4561149..4561565 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  I6H00_RS22955 (I6H00_22955) yqgE 4561565..4562128 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  I6H00_RS22960 (I6H00_22960) gshB 4562237..4563187 (-) 951 WP_000593273.1 glutathione synthase -
  I6H00_RS22965 (I6H00_22965) rsmE 4563200..4563931 (-) 732 WP_001222508.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  I6H00_RS22970 (I6H00_22970) endA 4564011..4564718 (-) 708 WP_000286510.1 deoxyribonuclease I -
  I6H00_RS22975 (I6H00_22975) yggI 4564813..4565310 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 34385.42 Da        Isoelectric Point: 5.3758

>NTDB_id=450347 I6H00_RS22945 WP_197939774.1 4559993..4560934(+) (pilT) [Escherichia coli strain FDAARGOS_943]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKNHQLPHVIQTGQQVGMLTF

Nucleotide


Download         Length: 942 bp        

>NTDB_id=450347 I6H00_RS22945 WP_197939774.1 4559993..4560934(+) (pilT) [Escherichia coli strain FDAARGOS_943]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATATCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAAACCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.476

100

0.508
  pilT Vibrio cholerae strain A1552

50.476

100

0.508
  pilT Neisseria meningitidis 8013

48.896

100

0.495
  pilT Neisseria gonorrhoeae MS11

48.58

100

0.492
  pilT Pseudomonas stutzeri DSM 10701

47.785

100

0.482
  pilT Acinetobacter baumannii D1279779

46.835

100

0.473
  pilT Acinetobacter baylyi ADP1

46.835

100

0.473
  pilT Acinetobacter nosocomialis M2

46.835

100

0.473
  pilT Acinetobacter baumannii strain A118

46.835

100

0.473
  pilT Pseudomonas aeruginosa PAK

46.835

100

0.473
  pilT Legionella pneumophila strain Lp02

46.032

100

0.463
  pilT Legionella pneumophila strain ERS1305867

46.032

100

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

100

0.425
  pilU Vibrio cholerae strain A1552

40.19

100

0.406
  pilU Pseudomonas stutzeri DSM 10701

38.801

100

0.393
  pilU Acinetobacter baylyi ADP1

37.383

100

0.383
  pilB Legionella pneumophila strain ERS1305867

30.997

100

0.367