Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CSE_RS07230 Genome accession   NC_017096
Coordinates   1427494..1428549 (+) Length   351 a.a.
NCBI ID   WP_041726633.1    Uniprot ID   -
Organism   Caldisericum exile AZM16c01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1422494..1433549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSE_RS07220 (CSE_14710) - 1423245..1425794 (+) 2550 WP_014453992.1 tetratricopeptide repeat protein -
  CSE_RS07225 (CSE_14720) - 1425791..1427494 (+) 1704 WP_014453993.1 GspE/PulE family protein -
  CSE_RS07230 (CSE_14730) pilT 1427494..1428549 (+) 1056 WP_041726633.1 type IV pilus twitching motility protein PilT Machinery gene
  CSE_RS07235 (CSE_14740) - 1428556..1429782 (+) 1227 WP_014453995.1 type II secretion system F family protein -
  CSE_RS07240 (CSE_14760) - 1430306..1430527 (-) 222 WP_014453997.1 type II toxin-antitoxin system HicA family toxin -
  CSE_RS07245 (CSE_14770) - 1430520..1430726 (-) 207 WP_014453998.1 type II toxin-antitoxin system HicB family antitoxin -
  CSE_RS07250 (CSE_14780) - 1431122..1432075 (-) 954 WP_014453999.1 ABC transporter permease -
  CSE_RS07255 (CSE_14790) - 1432072..1433196 (-) 1125 WP_014454000.1 ABC transporter permease -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39460.73 Da        Isoelectric Point: 6.4365

>NTDB_id=44807 CSE_RS07230 WP_041726633.1 1427494..1428549(+) (pilT) [Caldisericum exile AZM16c01]
MNINEILTLAADHKASDIHLTVGLPPVLRINKELVYLEADPLTPEDIAEMMYSIMTDKQKELFREKLEYDFSYGLKGLAR
FRVNVFMQRGSVAAAFRRIPFEIPPLDSLGVPKIAHKIIEEERGFVLVTGPTGHGKSTTLAAMIDEINMKFSKHIVTIED
PIEYLFRHKRSVVVQRELGSDTLSFPSALRAALREDPDVILVGEMRDLDTISTALTAAETGHLVFATLHTNSAAQSIDRI
VDVFPPHQQEQVKMQLSSVLLAIFSQQLIQKKDKKGLVLATEVLIATPAVRNLIREGKTHMIPSIIQTSSNVGMQTMEQS
LKELVLRGDITYEDGLRYAFNKENFVHLMER

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=44807 CSE_RS07230 WP_041726633.1 1427494..1428549(+) (pilT) [Caldisericum exile AZM16c01]
ATGAATATTAATGAAATTCTAACACTTGCAGCTGACCATAAAGCATCTGATATACATTTAACGGTTGGACTTCCACCCGT
TTTAAGGATAAACAAGGAACTTGTTTATCTTGAAGCAGACCCCCTTACACCTGAAGATATTGCAGAAATGATGTATTCGA
TAATGACAGATAAACAAAAGGAACTCTTTAGGGAAAAACTTGAATACGATTTTTCTTACGGCCTTAAAGGTCTTGCGCGT
TTCAGAGTTAACGTATTCATGCAAAGAGGATCAGTTGCGGCAGCATTTAGAAGAATTCCTTTTGAAATTCCGCCACTTGA
CTCACTTGGTGTCCCAAAGATTGCCCATAAAATAATTGAAGAAGAGCGAGGTTTCGTTCTTGTTACGGGTCCTACAGGGC
ATGGAAAATCAACAACACTTGCGGCAATGATTGACGAAATAAATATGAAATTTTCAAAGCATATCGTGACCATTGAAGAT
CCAATTGAATACCTCTTTAGGCACAAGCGGTCCGTGGTTGTTCAGAGAGAGCTTGGCTCAGACACCTTGTCGTTTCCCTC
AGCATTACGTGCAGCACTTCGTGAAGATCCAGATGTAATTCTTGTAGGAGAAATGAGAGACCTTGATACAATTTCAACTG
CCTTGACCGCAGCAGAAACAGGACATTTGGTTTTTGCAACACTGCATACGAACTCTGCTGCGCAATCCATTGATAGAATT
GTAGACGTTTTCCCTCCGCATCAACAAGAACAGGTAAAAATGCAACTTTCATCAGTTTTACTTGCAATATTCTCCCAACA
ACTCATACAAAAGAAAGACAAAAAGGGACTTGTTCTTGCAACAGAGGTACTCATTGCAACGCCTGCTGTAAGAAACCTCA
TAAGAGAAGGAAAGACTCATATGATTCCGTCAATAATTCAAACCTCATCAAATGTTGGTATGCAAACAATGGAACAATCT
CTTAAAGAACTCGTTCTAAGGGGAGACATAACATACGAAGATGGCCTAAGATACGCATTTAATAAAGAAAACTTTGTACA
TCTAATGGAAAGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.31

99.145

0.538
  pilT Neisseria meningitidis 8013

52.174

98.291

0.513
  pilT Neisseria gonorrhoeae MS11

51.884

98.291

0.51
  pilT Pseudomonas aeruginosa PAK

51.304

98.291

0.504
  pilT Acinetobacter baylyi ADP1

51.014

98.291

0.501
  pilT Pseudomonas stutzeri DSM 10701

50.725

98.291

0.499
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.049

93.447

0.496
  pilT Vibrio cholerae strain A1552

53.049

93.447

0.496
  pilT Acinetobacter baumannii strain A118

50.289

98.575

0.496
  pilT Acinetobacter baumannii D1279779

50.289

98.575

0.496
  pilT Acinetobacter nosocomialis M2

50.289

98.575

0.496
  pilT Legionella pneumophila strain ERS1305867

50.145

98.291

0.493
  pilT Legionella pneumophila strain Lp02

50.145

98.291

0.493
  pilU Pseudomonas stutzeri DSM 10701

42.693

99.43

0.424
  pilU Acinetobacter baylyi ADP1

42.197

98.575

0.416
  pilU Vibrio cholerae strain A1552

40.401

99.43

0.402

Multiple sequence alignment