Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HPY10_RS11395 Genome accession   NZ_CP053822
Coordinates   2551127..2552233 (-) Length   368 a.a.
NCBI ID   WP_175246224.1    Uniprot ID   -
Organism   Vibrio cholerae strain E7G     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2546127..2557233
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY10_RS11370 (HPY10_11420) gshB 2546740..2547696 (+) 957 WP_000593313.1 glutathione synthase -
  HPY10_RS11375 (HPY10_11425) - 2547732..2548295 (+) 564 WP_001054771.1 YqgE/AlgH family protein -
  HPY10_RS11380 (HPY10_11430) ruvX 2548376..2548798 (+) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  HPY10_RS11385 (HPY10_11435) tyrS 2548823..2550103 (-) 1281 WP_175246222.1 tyrosine--tRNA ligase -
  HPY10_RS11390 (HPY10_11440) - 2550212..2551009 (-) 798 WP_175246223.1 helix-turn-helix transcriptional regulator -
  HPY10_RS11395 (HPY10_11445) pilU 2551127..2552233 (-) 1107 WP_175246224.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HPY10_RS11400 (HPY10_11450) pilT 2552248..2553285 (-) 1038 WP_175246225.1 type IV pilus twitching motility protein PilT Machinery gene
  HPY10_RS11405 (HPY10_11455) - 2553311..2554021 (+) 711 WP_001256647.1 YggS family pyridoxal phosphate-dependent enzyme -
  HPY10_RS11410 (HPY10_11460) proC 2554073..2554891 (+) 819 WP_175246226.1 pyrroline-5-carboxylate reductase -
  HPY10_RS11415 (HPY10_11465) - 2554945..2555502 (+) 558 WP_001087261.1 YggT family protein -
  HPY10_RS11420 (HPY10_11470) yggU 2555502..2555792 (+) 291 WP_000741914.1 DUF167 family protein YggU -
  HPY10_RS11425 (HPY10_11475) - 2555838..2556269 (+) 432 WP_069731232.1 DUF4426 domain-containing protein -
  HPY10_RS11430 (HPY10_11480) - 2556339..2556941 (+) 603 WP_033930630.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41356.34 Da        Isoelectric Point: 6.4366

>NTDB_id=447903 HPY10_RS11395 WP_175246224.1 2551127..2552233(-) (pilU) [Vibrio cholerae strain E7G]
MELNQYLDGMLSHKASDLYITVGAPILYRVDGELRAQGEVLSLADVTTLLNAMMDDARQAEFKQTREANFAVVRDSGRFR
VSAFFQRELPGAVIRRIETRIPTFEELKLPEVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEIALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIAQQLLRDKNGKGRHGVFEVLLNSPRIADLIRRGELHELKATMARSQEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGDDYGSGSLQNVKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=447903 HPY10_RS11395 WP_175246224.1 2551127..2552233(-) (pilU) [Vibrio cholerae strain E7G]
ATGGAGTTAAATCAATATCTGGATGGGATGCTCAGCCATAAAGCATCGGATCTTTACATCACCGTGGGAGCGCCGATTTT
ATATCGAGTCGATGGTGAATTGCGTGCGCAAGGTGAAGTGCTCAGCTTGGCGGATGTCACCACCTTGTTGAATGCGATGA
TGGATGACGCGCGGCAAGCAGAATTTAAGCAGACGCGTGAAGCGAACTTTGCGGTGGTGCGTGACAGTGGTCGTTTTCGG
GTCAGCGCGTTTTTCCAACGTGAATTGCCGGGCGCGGTGATCCGCCGGATTGAAACGCGTATTCCCACCTTTGAAGAGCT
GAAATTGCCTGAAGTGCTGCAAAATTTAGCGATCGCGAAACGCGGTTTAGTCTTAGTAGTGGGTGCTACGGGGTCAGGTA
AGTCGACCACTATGGCGGCAATGACGGGGTATCGTAATCAGCACCGTACCGGACATATCTTGACAGTTGAAGATCCGATT
GAGTTTGTGCATGAACACAAGCGCTGCATCGTGACTCAACGAGAAGTCGGGCTCGATACTGAAAGCTATGAAATCGCGCT
GAAAAACTCGCTGCGCCAAGCGCCGGATATGATTTTGATTGGTGAAATCCGCAGCCGAGAAACCATGGAATACGCGATGA
CTTTCGCGGAAACCGGGCATTTGTGTATGGCCACGCTGCACGCCAACAATGCTAACCAAGCGTTAGAGCGGATTTTGCAT
TTAGTGCCGAAAGAGCAGCGTGAGCAGTTTTTGCTCGATTTGTCACTCAACCTCAAAGGCGTGATTGCCCAGCAATTATT
GCGCGATAAAAATGGCAAAGGTCGACACGGGGTGTTTGAAGTGTTGCTCAATAGCCCGCGTATCGCGGATTTAATTCGCC
GCGGTGAACTGCATGAGCTGAAAGCGACCATGGCTCGTTCGCAGGAAGTGGGGATGCAAACCTTTGATCAAGCCTTATAC
CAGCTTGTGGTGGATGACAAAATCAGTGAGCAAGATGCCCTGCACAGTGCCGATTCAGCCAACGATCTGCGTTTGATGCT
CAAAACCAAGCGTGGTGATGACTACGGCAGCGGCTCTTTGCAGAATGTGAAGATTGATATGGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

98.37

100

0.984
  pilU Pseudomonas stutzeri DSM 10701

58.286

95.109

0.554
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Legionella pneumophila strain ERS1305867

42.773

92.12

0.394
  pilT Legionella pneumophila strain Lp02

42.773

92.12

0.394
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.806

84.239

0.386
  pilT Acinetobacter baumannii D1279779

41.298

92.12

0.38
  pilT Acinetobacter baumannii strain A118

41.298

92.12

0.38
  pilT Acinetobacter nosocomialis M2

41.298

92.12

0.38
  pilT Vibrio cholerae strain A1552

41.916

90.761

0.38
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.916

90.761

0.38
  pilT Pseudomonas aeruginosa PAK

41.003

92.12

0.378
  pilT Acinetobacter baylyi ADP1

42.154

88.315

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.413

92.12

0.372
  pilT Neisseria gonorrhoeae MS11

38.329

94.293

0.361
  pilT Neisseria meningitidis 8013

38.551

93.75

0.361