Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HPY10_RS11400 Genome accession   NZ_CP053822
Coordinates   2552248..2553285 (-) Length   345 a.a.
NCBI ID   WP_175246225.1    Uniprot ID   -
Organism   Vibrio cholerae strain E7G     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2547248..2558285
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY10_RS11375 (HPY10_11425) - 2547732..2548295 (+) 564 WP_001054771.1 YqgE/AlgH family protein -
  HPY10_RS11380 (HPY10_11430) ruvX 2548376..2548798 (+) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  HPY10_RS11385 (HPY10_11435) tyrS 2548823..2550103 (-) 1281 WP_175246222.1 tyrosine--tRNA ligase -
  HPY10_RS11390 (HPY10_11440) - 2550212..2551009 (-) 798 WP_175246223.1 helix-turn-helix transcriptional regulator -
  HPY10_RS11395 (HPY10_11445) pilU 2551127..2552233 (-) 1107 WP_175246224.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HPY10_RS11400 (HPY10_11450) pilT 2552248..2553285 (-) 1038 WP_175246225.1 type IV pilus twitching motility protein PilT Machinery gene
  HPY10_RS11405 (HPY10_11455) - 2553311..2554021 (+) 711 WP_001256647.1 YggS family pyridoxal phosphate-dependent enzyme -
  HPY10_RS11410 (HPY10_11460) proC 2554073..2554891 (+) 819 WP_175246226.1 pyrroline-5-carboxylate reductase -
  HPY10_RS11415 (HPY10_11465) - 2554945..2555502 (+) 558 WP_001087261.1 YggT family protein -
  HPY10_RS11420 (HPY10_11470) yggU 2555502..2555792 (+) 291 WP_000741914.1 DUF167 family protein YggU -
  HPY10_RS11425 (HPY10_11475) - 2555838..2556269 (+) 432 WP_069731232.1 DUF4426 domain-containing protein -
  HPY10_RS11430 (HPY10_11480) - 2556339..2556941 (+) 603 WP_033930630.1 XTP/dITP diphosphatase -
  HPY10_RS11435 (HPY10_11485) hemW 2556935..2558110 (+) 1176 WP_175246227.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38277.91 Da        Isoelectric Point: 6.6672

>NTDB_id=447904 HPY10_RS11400 WP_175246225.1 2552248..2553285(-) (pilT) [Vibrio cholerae strain E7G]
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHSDVHRLIFEIMNDAQRSEYEEKLEVDFSFELPNVGR
FRVNAFHQARGCSAVFRTIPTVIPTLEQLDAPEIFSKIANYEKGLVLVTGPTGSGKSTTLAAMVNYVNAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMVRGVVDAQEVQSKIELDLKAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=447904 HPY10_RS11400 WP_175246225.1 2552248..2553285(-) (pilT) [Vibrio cholerae strain E7G]
ATGGATATCGCTGAGTTACTGGAATTTAGTGTAAAACATAACGCCTCAGATCTACATCTTTCCGCAGGTGTTCCGCCTAT
GGTACGGATTGATGGCGAAGTTAGGAAGCTTGGCGTACCTGCTTTTACCCATTCTGATGTACATCGCTTGATTTTTGAGA
TCATGAATGATGCCCAGCGCAGTGAATATGAAGAGAAATTAGAAGTCGATTTTTCTTTTGAACTGCCTAATGTTGGCCGT
TTCCGGGTTAACGCGTTTCATCAAGCGCGCGGATGCTCGGCGGTGTTTCGTACCATTCCCACTGTTATCCCGACGTTAGA
GCAACTGGATGCTCCTGAGATCTTCAGCAAAATTGCCAATTATGAAAAAGGGTTAGTTTTAGTGACTGGGCCAACGGGGT
CGGGTAAGTCGACGACCTTGGCGGCGATGGTGAACTATGTCAATGCCCATCACAATAAGCATATTTTGACTATTGAAGAT
CCGATTGAATTTGTGCACAGCAACAATAAGTGTTTGATCAACCAACGCGAAGTGCACCGTGATACCCACAGTTTTAAAAA
TGCATTGCGCTCGGCATTGCGTGAAGACCCAGATGTGATTTTGGTTGGTGAGCTGCGTGACCAAGAAACGATTAGCTTGG
CGCTTACCGCAGCGGAAACCGGTCACTTGGTGTTTGGTACACTGCACACCAGCTCGGCGGCAAAAACCATTGACCGGATT
ATTGATGTGTTTCCGGGCAGCGATAAAGACATGGTGCGTTCAATGCTGTCTGAATCGCTGCGTGCGGTTATCGCGCAAAA
GCTCTTAAAACGCGTGGGTGGTGGCCGCGTTGCGTGCCATGAAATCATGCTGGCGACTCCGGCAATCCGGAACTTGATCC
GTGAAGATAAAGTGGCGCAGATGTATTCGATCATCCAAACCGGTGCCGCACATGGTATGCAAACCATGGAGCAAAATGCC
AAACAGCTGATGGTGCGTGGTGTGGTCGATGCGCAAGAAGTGCAGAGCAAAATCGAGTTGGATTTAAAAGCATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

99.42

100

0.994
  pilT Vibrio cholerae strain A1552

99.42

100

0.994
  pilT Acinetobacter baumannii D1279779

71.429

97.391

0.696
  pilT Acinetobacter baumannii strain A118

71.429

97.391

0.696
  pilT Acinetobacter nosocomialis M2

71.429

97.391

0.696
  pilT Acinetobacter baylyi ADP1

72.121

95.652

0.69
  pilT Pseudomonas stutzeri DSM 10701

68.546

97.681

0.67
  pilT Pseudomonas aeruginosa PAK

66.471

98.551

0.655
  pilT Legionella pneumophila strain Lp02

67.477

95.362

0.643
  pilT Legionella pneumophila strain ERS1305867

67.477

95.362

0.643
  pilT Neisseria meningitidis 8013

64.545

95.652

0.617
  pilT Neisseria gonorrhoeae MS11

64.242

95.652

0.614
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.988

94.783

0.493
  pilU Vibrio cholerae strain A1552

42.515

96.812

0.412
  pilU Pseudomonas stutzeri DSM 10701

41.124

97.971

0.403
  pilU Acinetobacter baylyi ADP1

40.625

92.754

0.377