Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AMIS_RS04550 Genome accession   NC_017093
Coordinates   965642..966760 (-) Length   372 a.a.
NCBI ID   WP_014441022.1    Uniprot ID   -
Organism   Actinoplanes missouriensis 431     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 960642..971760
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AMIS_RS04520 (AMIS_8990) pilO 960667..961281 (-) 615 WP_014441016.1 type 4a pilus biogenesis protein PilO -
  AMIS_RS04525 (AMIS_9000) - 961281..961979 (-) 699 WP_014441017.1 hypothetical protein -
  AMIS_RS04530 (AMIS_9010) pilM 961976..963001 (-) 1026 WP_014441018.1 type IV pilus assembly protein PilM -
  AMIS_RS04535 (AMIS_9020) - 963023..963802 (-) 780 WP_014441019.1 A24 family peptidase -
  AMIS_RS04540 (AMIS_9030) - 963841..964317 (-) 477 WP_014441020.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AMIS_RS04545 (AMIS_9040) - 964405..965637 (-) 1233 WP_014441021.1 type II secretion system F family protein -
  AMIS_RS04550 (AMIS_9050) pilT 965642..966760 (-) 1119 WP_014441022.1 type IV pilus twitching motility protein PilT Machinery gene
  AMIS_RS04555 (AMIS_9060) - 966843..968504 (-) 1662 WP_041830505.1 GspE/PulE family protein -
  AMIS_RS04560 (AMIS_9070) - 968550..969506 (-) 957 WP_014441024.1 GlxA family transcriptional regulator -
  AMIS_RS04565 (AMIS_9080) - 969573..970955 (+) 1383 WP_083888565.1 MFS transporter -
  AMIS_RS04570 (AMIS_9090) - 970945..971262 (-) 318 WP_014441026.1 STAS domain-containing protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 40355.12 Da        Isoelectric Point: 6.7434

>NTDB_id=44774 AMIS_RS04550 WP_014441022.1 965642..966760(-) (pilT) [Actinoplanes missouriensis 431]
MDTIEQVRPPETAATSDDLDVLDQLLVTLVESRGSDLHLTVGSPPMMRVDGGLRPVPGYGKLNASDTELLARAAVTPAQW
ETFQRVQEMDFAHSIVGVSRFRGNLYRQRASCGAVFRAIPHKIKPLDELGMPESVARFAHLPRGLVLVTGPTGSGKTTTL
ASLLDLANRSRANHIITIEDPIEFLHPHKRSVVNQREVGSDTENFATALKHALRQDPDIILVGELRDLETTATALTAAET
GHLVLATLHTQSATQTIDRVIDIFPPHQQLQIRAQLAASLQGVITQALAPKADGKGRVVVAEILTATPAIRSLIREGKSH
QIPSFMQAGASEGMLAFDQHLAEKIREGAITMQTALEISHSPDELKRLVGRA

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=44774 AMIS_RS04550 WP_014441022.1 965642..966760(-) (pilT) [Actinoplanes missouriensis 431]
ATGGACACGATCGAGCAGGTTCGGCCGCCCGAGACGGCCGCCACCTCGGACGATCTGGACGTGCTCGACCAGTTGCTCGT
CACTCTGGTGGAGTCACGCGGCTCCGACCTGCACCTGACCGTCGGCTCGCCGCCGATGATGCGGGTCGACGGCGGGCTGC
GGCCGGTGCCCGGCTACGGCAAGCTGAACGCCTCGGACACCGAGCTGCTGGCCCGCGCGGCGGTCACCCCGGCGCAGTGG
GAGACATTCCAGCGCGTCCAGGAGATGGACTTCGCGCACAGCATCGTCGGCGTCTCCCGGTTCCGCGGCAACCTCTACCG
GCAGCGGGCCTCCTGCGGCGCGGTCTTCCGGGCCATCCCACACAAGATCAAACCGCTGGACGAGCTCGGCATGCCGGAGT
CGGTGGCCCGTTTCGCCCACCTGCCCCGCGGCCTGGTGCTGGTGACCGGCCCGACCGGTTCCGGCAAGACCACCACGCTG
GCCTCACTGCTCGACCTGGCGAACCGGAGCCGGGCCAACCACATCATCACGATCGAGGACCCGATCGAGTTCCTGCACCC
GCACAAGCGCAGCGTGGTCAACCAGCGCGAGGTCGGCTCGGACACCGAGAACTTCGCCACCGCGCTCAAGCACGCGCTAC
GGCAGGACCCGGACATCATCCTGGTCGGCGAGCTGCGTGACCTGGAGACGACCGCGACCGCGCTGACCGCCGCGGAGACC
GGTCACCTGGTGCTGGCGACGCTGCACACGCAGAGCGCAACCCAGACCATCGATCGGGTCATCGACATCTTCCCGCCGCA
CCAGCAGCTGCAGATCCGCGCCCAGCTGGCCGCCAGCCTGCAGGGCGTCATCACCCAGGCGCTGGCGCCGAAGGCCGACG
GCAAGGGGCGGGTGGTGGTCGCCGAGATCCTCACCGCGACCCCGGCCATCCGCAGCCTGATCCGCGAAGGCAAGTCGCAC
CAGATCCCGTCGTTCATGCAGGCCGGCGCGAGCGAGGGCATGCTCGCCTTCGATCAGCACCTGGCCGAGAAGATTCGGGA
GGGCGCGATCACCATGCAGACCGCGCTGGAGATCAGCCACTCACCGGACGAGCTCAAGCGGCTCGTGGGACGGGCGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(131-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.704

95.43

0.484
  pilT Pseudomonas aeruginosa PAK

49.709

92.473

0.46
  pilT Acinetobacter nosocomialis M2

49.123

91.935

0.452
  pilT Acinetobacter baumannii D1279779

49.123

91.935

0.452
  pilT Acinetobacter baumannii strain A118

49.123

91.935

0.452
  pilT Pseudomonas stutzeri DSM 10701

49.123

91.935

0.452
  pilT Acinetobacter baylyi ADP1

49.123

91.935

0.452
  pilT Legionella pneumophila strain ERS1305867

49.401

89.785

0.444
  pilT Legionella pneumophila strain Lp02

49.401

89.785

0.444
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.485

88.71

0.43
  pilT Vibrio cholerae strain A1552

48.485

88.71

0.43
  pilT Neisseria meningitidis 8013

46.224

88.978

0.411
  pilT Neisseria gonorrhoeae MS11

46.224

88.978

0.411
  pilU Vibrio cholerae strain A1552

42.241

93.548

0.395
  pilU Pseudomonas stutzeri DSM 10701

41.092

93.548

0.384
  pilU Acinetobacter baylyi ADP1

38.793

93.548

0.363

Multiple sequence alignment