Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HPY07_RS11485 Genome accession   NZ_CP053806
Coordinates   2559463..2560500 (-) Length   345 a.a.
NCBI ID   WP_071187324.1    Uniprot ID   -
Organism   Vibrio cholerae strain SP6G     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2554463..2565500
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY07_RS11460 (HPY07_11470) - 2554947..2555510 (+) 564 WP_001054771.1 YqgE/AlgH family protein -
  HPY07_RS11465 (HPY07_11475) ruvX 2555577..2555999 (+) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  HPY07_RS11470 (HPY07_11480) tyrS 2556038..2557318 (-) 1281 WP_162806855.1 tyrosine--tRNA ligase -
  HPY07_RS11475 (HPY07_11485) - 2557427..2558224 (-) 798 WP_162806856.1 helix-turn-helix transcriptional regulator -
  HPY07_RS11480 (HPY07_11490) pilU 2558342..2559448 (-) 1107 WP_175242132.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HPY07_RS11485 (HPY07_11495) pilT 2559463..2560500 (-) 1038 WP_071187324.1 type IV pilus twitching motility protein PilT Machinery gene
  HPY07_RS11490 (HPY07_11500) - 2560526..2561236 (+) 711 WP_071187322.1 YggS family pyridoxal phosphate-dependent enzyme -
  HPY07_RS11495 (HPY07_11505) proC 2561289..2562107 (+) 819 WP_000437941.1 pyrroline-5-carboxylate reductase -
  HPY07_RS11500 (HPY07_11510) - 2562161..2562718 (+) 558 WP_001087261.1 YggT family protein -
  HPY07_RS11505 (HPY07_11515) yggU 2562718..2563008 (+) 291 WP_071180105.1 DUF167 family protein YggU -
  HPY07_RS11510 (HPY07_11520) - 2563054..2563485 (+) 432 WP_001233674.1 DUF4426 domain-containing protein -
  HPY07_RS11515 (HPY07_11525) - 2563555..2564157 (+) 603 WP_044126943.1 XTP/dITP diphosphatase -
  HPY07_RS11520 (HPY07_11530) hemW 2564151..2565326 (+) 1176 WP_175242133.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38232.81 Da        Isoelectric Point: 6.5572

>NTDB_id=447620 HPY07_RS11485 WP_071187324.1 2559463..2560500(-) (pilT) [Vibrio cholerae strain SP6G]
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHSDVHRLVFEIMNDAQRSEYEEKLEVDFSFELPNVGR
FRVNAFHQARGCSAVFRTIPTVIPTLEQLDAPEIFTKIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIARGVVDAQEVQSKIELDLKAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=447620 HPY07_RS11485 WP_071187324.1 2559463..2560500(-) (pilT) [Vibrio cholerae strain SP6G]
ATGGATATCGCTGAGTTACTGGAATTTAGTGTAAAACATAACGCCTCAGATCTACATCTTTCCGCCGGTGTTCCGCCTAT
GGTACGGATTGATGGTGAAGTTAGGAAGCTTGGCGTGCCTGCTTTTACCCATTCTGACGTACATCGCTTGGTATTTGAAA
TAATGAATGATGCCCAGCGCAGTGAATATGAAGAAAAATTAGAAGTCGATTTTTCTTTTGAACTGCCTAATGTTGGCCGC
TTCCGGGTTAATGCGTTTCATCAAGCGCGCGGATGCTCTGCGGTGTTTCGTACCATTCCGACCGTGATCCCAACCTTAGA
GCAATTAGATGCACCTGAGATTTTCACCAAGATCGCCAACTATGAAAAAGGCTTAGTGTTGGTCACCGGACCTACCGGTT
CGGGTAAATCCACCACGCTCGCGGCGATGGTCGATTACGTGAATGCTCATCACAACAAGCATATCTTGACCATTGAAGAT
CCGATTGAATTTGTGCACAGCAACAATAAGTGTTTGATCAACCAACGCGAAGTACACCGTGATACTCACAGTTTTAAAAA
TGCCCTTCGCTCGGCATTGCGTGAAGACCCTGATGTGATTCTGGTTGGTGAGCTGCGTGACCAAGAAACCATTAGCTTGG
CGCTTACCGCAGCGGAAACCGGTCACTTGGTGTTTGGTACACTGCACACCAGCTCGGCGGCAAAAACCATTGACCGGATT
ATCGATGTTTTTCCGGGCAGCGACAAAGACATGGTGCGTTCCATGCTGTCGGAATCGCTGCGTGCGGTTATTGCGCAAAA
GCTCCTAAAACGCGTCGGGGGGGGCCGTGTCGCGTGTCATGAAATCATGCTGGCAACGCCAGCGATCCGGAACTTGATCC
GTGAAGATAAAGTGGCGCAGATGTATTCGATCATCCAAACCGGTGCCGCACACGGCATGCAAACCATGGAGCAAAACGCC
AAACAGCTGATCGCTCGTGGTGTGGTGGATGCGCAAGAAGTACAGAGCAAAATAGAGTTGGACTTAAAAGCATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

99.13

100

0.991
  pilT Vibrio cholerae strain A1552

99.13

100

0.991
  pilT Acinetobacter baumannii D1279779

72.321

97.391

0.704
  pilT Acinetobacter baumannii strain A118

72.321

97.391

0.704
  pilT Acinetobacter nosocomialis M2

72.024

97.391

0.701
  pilT Acinetobacter baylyi ADP1

72.424

95.652

0.693
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.681

0.672
  pilT Pseudomonas aeruginosa PAK

66.765

98.551

0.658
  pilT Legionella pneumophila strain Lp02

67.477

95.362

0.643
  pilT Legionella pneumophila strain ERS1305867

67.477

95.362

0.643
  pilT Neisseria meningitidis 8013

64.545

95.652

0.617
  pilT Neisseria gonorrhoeae MS11

64.242

95.652

0.614
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.294

94.783

0.496
  pilU Vibrio cholerae strain A1552

42.216

96.812

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.419

96.812

0.391
  pilU Acinetobacter baylyi ADP1

40.312

92.754

0.374