Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HPY07_RS11480 Genome accession   NZ_CP053806
Coordinates   2558342..2559448 (-) Length   368 a.a.
NCBI ID   WP_175242132.1    Uniprot ID   -
Organism   Vibrio cholerae strain SP6G     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2553342..2564448
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY07_RS11455 (HPY07_11465) gshB 2553955..2554911 (+) 957 WP_000593313.1 glutathione synthase -
  HPY07_RS11460 (HPY07_11470) - 2554947..2555510 (+) 564 WP_001054771.1 YqgE/AlgH family protein -
  HPY07_RS11465 (HPY07_11475) ruvX 2555577..2555999 (+) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  HPY07_RS11470 (HPY07_11480) tyrS 2556038..2557318 (-) 1281 WP_162806855.1 tyrosine--tRNA ligase -
  HPY07_RS11475 (HPY07_11485) - 2557427..2558224 (-) 798 WP_162806856.1 helix-turn-helix transcriptional regulator -
  HPY07_RS11480 (HPY07_11490) pilU 2558342..2559448 (-) 1107 WP_175242132.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HPY07_RS11485 (HPY07_11495) pilT 2559463..2560500 (-) 1038 WP_071187324.1 type IV pilus twitching motility protein PilT Machinery gene
  HPY07_RS11490 (HPY07_11500) - 2560526..2561236 (+) 711 WP_071187322.1 YggS family pyridoxal phosphate-dependent enzyme -
  HPY07_RS11495 (HPY07_11505) proC 2561289..2562107 (+) 819 WP_000437941.1 pyrroline-5-carboxylate reductase -
  HPY07_RS11500 (HPY07_11510) - 2562161..2562718 (+) 558 WP_001087261.1 YggT family protein -
  HPY07_RS11505 (HPY07_11515) yggU 2562718..2563008 (+) 291 WP_071180105.1 DUF167 family protein YggU -
  HPY07_RS11510 (HPY07_11520) - 2563054..2563485 (+) 432 WP_001233674.1 DUF4426 domain-containing protein -
  HPY07_RS11515 (HPY07_11525) - 2563555..2564157 (+) 603 WP_044126943.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41316.28 Da        Isoelectric Point: 6.5689

>NTDB_id=447619 HPY07_RS11480 WP_175242132.1 2558342..2559448(-) (pilU) [Vibrio cholerae strain SP6G]
MELNQYLDGMLTHKASDLYITVGAPILYRVDGELRAQGEALSVADVTALLHAMMDDARQAEFKQTREANFAVVRDSGRFR
VSAFFQRELPGAVIRRIETRIPTFEELKLPEVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIAQQLLRDKNGKGRHGVFEVLLNSPRIADLIRRGELHELKATMARSHEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGDDYGSGSLQNVKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=447619 HPY07_RS11480 WP_175242132.1 2558342..2559448(-) (pilU) [Vibrio cholerae strain SP6G]
ATGGAGTTGAATCAATATCTGGATGGCATGCTGACTCATAAAGCATCGGATCTGTACATTACGGTGGGTGCACCGATTTT
GTATCGAGTCGATGGTGAATTGCGTGCGCAAGGTGAAGCGCTCAGCGTGGCGGATGTGACGGCCTTATTGCATGCGATGA
TGGATGACGCAAGGCAAGCAGAATTTAAGCAGACGCGCGAGGCGAATTTTGCTGTGGTGCGTGACAGTGGCCGTTTTCGG
GTGAGTGCATTTTTCCAACGTGAATTGCCGGGGGCGGTGATCCGCCGGATTGAAACGCGCATTCCCACCTTTGAAGAGCT
GAAATTGCCTGAAGTGCTGCAAAATTTGGCGATTGCCAAACGCGGTTTAGTGTTGGTGGTCGGTGCAACCGGTTCGGGGA
AATCGACCACTATGGCAGCCATGACTGGCTATCGTAATCAGCATCGCACCGGGCATATTTTGACGGTCGAAGATCCCATT
GAGTTTGTGCATGAACACAAGCGCTGTATCGTGACGCAGCGAGAAGTGGGGCTCGATACCGAAAGCTATGAAGTGGCGCT
GAAAAACTCGCTGCGCCAAGCCCCCGATATGATTTTGATTGGTGAAATCCGCAGCCGAGAAACCATGGAATACGCGATGA
CTTTCGCGGAAACCGGGCATTTGTGTATGGCCACGCTGCACGCCAATAACGCCAACCAAGCGTTAGAGCGGATTTTGCAT
TTAGTGCCGAAAGAGCAGCGTGAGCAGTTTTTGCTCGATTTGTCACTCAACCTCAAAGGCGTGATTGCCCAGCAATTATT
GCGCGATAAAAATGGCAAAGGTCGACACGGGGTGTTTGAAGTGTTGCTCAATAGCCCGCGTATCGCGGATTTAATTCGCC
GCGGTGAACTGCATGAGCTGAAAGCGACCATGGCTCGTTCGCACGAAGTGGGGATGCAAACTTTCGATCAAGCCTTATAC
CAGCTTGTGGTGGATGACAAAATCAGTGAGCAAGATGCCCTGCACAGTGCCGATTCAGCCAACGATCTGCGTTTGATGCT
CAAAACCAAGCGTGGTGATGACTACGGCAGCGGCTCTTTGCAGAATGTGAAGATTGATATGGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

99.728

100

0.997
  pilU Pseudomonas stutzeri DSM 10701

58.857

95.109

0.56
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Legionella pneumophila strain ERS1305867

42.478

92.12

0.391
  pilT Legionella pneumophila strain Lp02

42.478

92.12

0.391
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.806

84.239

0.386
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.216

90.761

0.383
  pilT Vibrio cholerae strain A1552

42.216

90.761

0.383
  pilT Acinetobacter baumannii D1279779

41.593

92.12

0.383
  pilT Acinetobacter baumannii strain A118

41.593

92.12

0.383
  pilT Acinetobacter nosocomialis M2

41.593

92.12

0.383
  pilT Pseudomonas aeruginosa PAK

41.298

92.12

0.38
  pilT Pseudomonas stutzeri DSM 10701

41.003

92.12

0.378
  pilT Acinetobacter baylyi ADP1

42.462

88.315

0.375