Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RGE_RS00385 Genome accession   NC_017075
Coordinates   81222..82265 (-) Length   347 a.a.
NCBI ID   WP_014426316.1    Uniprot ID   I0HK87
Organism   Rubrivivax gelatinosus IL144     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 76222..87265
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RGE_RS00355 (RGE_00730) - 76786..77172 (+) 387 WP_014426310.1 YraN family protein -
  RGE_RS00360 (RGE_00740) - 77224..77781 (+) 558 WP_014426311.1 SIS domain-containing protein -
  RGE_RS00365 (RGE_00750) - 77796..78428 (+) 633 WP_043783641.1 BON domain-containing protein -
  RGE_RS00370 (RGE_00760) - 78463..79353 (-) 891 WP_014426313.1 NAD(P)-dependent oxidoreductase -
  RGE_RS00375 (RGE_00770) pilU 79402..80538 (-) 1137 WP_014426314.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RGE_RS00380 (RGE_00780) - 80558..81079 (-) 522 WP_148280091.1 cyclic nucleotide-binding domain-containing protein -
  RGE_RS00385 (RGE_00790) pilT 81222..82265 (-) 1044 WP_014426316.1 type IV pilus twitching motility protein PilT Machinery gene
  RGE_RS00390 (RGE_00800) - 82309..83001 (+) 693 WP_014426317.1 YggS family pyridoxal phosphate-dependent enzyme -
  RGE_RS00395 (RGE_00810) - 83012..83221 (-) 210 WP_014426318.1 hypothetical protein -
  RGE_RS00400 (RGE_00820) - 83361..83861 (-) 501 WP_014426319.1 Lrp/AsnC ligand binding domain-containing protein -
  RGE_RS00405 (RGE_00830) - 83955..86954 (+) 3000 WP_014426320.1 L-glutamate gamma-semialdehyde dehydrogenase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38107.93 Da        Isoelectric Point: 7.3203

>NTDB_id=44728 RGE_RS00385 WP_014426316.1 81222..82265(-) (pilT) [Rubrivivax gelatinosus IL144]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVEPLDHKMVHAMVYDIMNDAQRKHYEDTLECDFSFEIQGLAR
FRVNAFNQNRGAGAVFRTIPSKILTLEQLGTPKVFSELALKPRGLVLVTGPTGSGKSTTLAAMINHLNENEYGHVLTVED
PIEFVHESKKCLVNQREVGPHTLSFANALRSALREDPDAILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPAAEKEMVRAMLSESLVAVISQTLCKLKDGSGRVAAHEIMIATSAIKNLIRENKIAQMYSAIQTGQSLGMQTLDQN
LADLVRRNVITPAEARAKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=44728 RGE_RS00385 WP_014426316.1 81222..82265(-) (pilT) [Rubrivivax gelatinosus IL144]
ATGGACATCACCCAGCTGCTCGCATTTTCGGTCAAGAACAAGGCGTCCGACCTGCACCTGTCGGCCGGGCTGCCGCCGAT
GATCCGCGTGCACGGGGACGTGCGTCGCATCAATGTCGAACCGCTGGACCACAAGATGGTCCACGCGATGGTCTACGACA
TCATGAACGACGCCCAGCGCAAGCACTACGAGGACACGCTGGAGTGCGACTTCAGCTTCGAGATCCAGGGCCTCGCACGT
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGCACGATCCCGAGCAAGATCCTGACGCTGGA
GCAGCTGGGCACGCCCAAGGTCTTCTCCGAGCTGGCGCTCAAGCCGCGCGGCCTGGTGCTCGTCACCGGCCCCACCGGCT
CGGGCAAGAGCACTACGCTGGCGGCGATGATCAACCACCTCAACGAGAACGAGTACGGCCATGTGCTCACCGTCGAGGAC
CCGATCGAGTTCGTGCACGAGAGCAAGAAGTGCCTCGTCAACCAGCGCGAGGTCGGCCCGCACACGCTCTCCTTCGCCAA
CGCGCTGCGCTCGGCGCTGCGCGAGGACCCGGACGCGATCCTGGTCGGCGAAATGCGCGACCTGGAGACGATCCGCCTGG
CGCTGACCGCCGCCGAAACCGGCCACCTGGTCTTCGGCACGCTGCACACCTCCAGCGCCGCCAAGACCATCGACCGCGTC
GTCGACGTCTTCCCCGCGGCCGAGAAGGAGATGGTGCGCGCGATGCTGTCCGAGTCGCTGGTCGCCGTCATCTCGCAGAC
GCTGTGCAAGCTCAAGGACGGCTCGGGCCGCGTCGCCGCGCACGAGATCATGATCGCCACCTCGGCGATCAAGAACCTGA
TCCGCGAGAACAAGATCGCCCAGATGTACTCGGCCATCCAGACCGGCCAGAGCCTGGGCATGCAGACGCTGGACCAGAAC
CTCGCCGACCTGGTGCGCCGCAACGTCATCACGCCCGCCGAGGCGCGCGCGAAGGCGAAGATTCCTGAGAACTTCCCCGG
GTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I0HK87

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.681

99.424

0.772
  pilT Pseudomonas stutzeri DSM 10701

76.232

99.424

0.758
  pilT Acinetobacter baumannii D1279779

75.942

99.424

0.755
  pilT Acinetobacter baumannii strain A118

75.942

99.424

0.755
  pilT Acinetobacter nosocomialis M2

75.942

99.424

0.755
  pilT Acinetobacter baylyi ADP1

73.333

99.424

0.729
  pilT Legionella pneumophila strain Lp02

71.976

97.695

0.703
  pilT Legionella pneumophila strain ERS1305867

71.976

97.695

0.703
  pilT Neisseria meningitidis 8013

68.696

99.424

0.683
  pilT Neisseria gonorrhoeae MS11

68.406

99.424

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.689

98.271

0.646
  pilT Vibrio cholerae strain A1552

65.689

98.271

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

99.424

0.513
  pilU Pseudomonas stutzeri DSM 10701

42.687

96.542

0.412
  pilU Vibrio cholerae strain A1552

42.687

96.542

0.412
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412

Multiple sequence alignment