Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RGE_RS00375 Genome accession   NC_017075
Coordinates   79402..80538 (-) Length   378 a.a.
NCBI ID   WP_014426314.1    Uniprot ID   -
Organism   Rubrivivax gelatinosus IL144     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 74402..85538
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RGE_RS00345 (RGE_00710) - 75642..75887 (+) 246 WP_014426308.1 hypothetical protein -
  RGE_RS00350 (RGE_00720) rsmI 75894..76793 (-) 900 WP_014426309.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  RGE_RS00355 (RGE_00730) - 76786..77172 (+) 387 WP_014426310.1 YraN family protein -
  RGE_RS00360 (RGE_00740) - 77224..77781 (+) 558 WP_014426311.1 SIS domain-containing protein -
  RGE_RS00365 (RGE_00750) - 77796..78428 (+) 633 WP_043783641.1 BON domain-containing protein -
  RGE_RS00370 (RGE_00760) - 78463..79353 (-) 891 WP_014426313.1 NAD(P)-dependent oxidoreductase -
  RGE_RS00375 (RGE_00770) pilU 79402..80538 (-) 1137 WP_014426314.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RGE_RS00380 (RGE_00780) - 80558..81079 (-) 522 WP_148280091.1 cyclic nucleotide-binding domain-containing protein -
  RGE_RS00385 (RGE_00790) pilT 81222..82265 (-) 1044 WP_014426316.1 type IV pilus twitching motility protein PilT Machinery gene
  RGE_RS00390 (RGE_00800) - 82309..83001 (+) 693 WP_014426317.1 YggS family pyridoxal phosphate-dependent enzyme -
  RGE_RS00395 (RGE_00810) - 83012..83221 (-) 210 WP_014426318.1 hypothetical protein -
  RGE_RS00400 (RGE_00820) - 83361..83861 (-) 501 WP_014426319.1 Lrp/AsnC ligand binding domain-containing protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42199.47 Da        Isoelectric Point: 6.9138

>NTDB_id=44727 RGE_RS00375 WP_014426314.1 79402..80538(-) (pilU) [Rubrivivax gelatinosus IL144]
MERDQASKFVNDLLRLMITRSGSDLFLTADFPPAIKVDGKVTKVSPQPLTGQHTLQLARAVMNDKQAAEFERTKECNFAI
SPQGVGRFRVNAFMQQGQVGLVLRTIPQTLPTIDTLGLPGVLKDIAMTKRGLVIFVGATGSGKSTSLAAMVDWRNEHSYG
HIITVEDPIEFVHPHKNCIVTQREVGVDTDGWEQALKNTLRQAPDVILMGEVRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRGQLLMDLSLNLKALVSQRLMPRQEGRGRVAAVEIMLNTPLVSDMIFKGEIGEIKELMKRSRELG
MQTFDQSLFDLYEANAITYEDALRNADSVNDLRLQIKLNSQRARSRDLSAGTENMRVI

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=44727 RGE_RS00375 WP_014426314.1 79402..80538(-) (pilU) [Rubrivivax gelatinosus IL144]
ATGGAACGCGACCAGGCCTCGAAATTCGTCAACGATCTGCTGCGGCTGATGATCACGCGCAGCGGCTCGGACCTCTTTCT
CACCGCCGACTTCCCGCCGGCGATCAAGGTCGACGGCAAGGTGACCAAGGTCTCGCCGCAGCCGCTGACCGGCCAGCACA
CGCTGCAGCTGGCGCGCGCGGTGATGAACGACAAGCAGGCCGCCGAGTTCGAGCGCACCAAGGAGTGCAACTTCGCGATC
AGCCCGCAGGGCGTGGGGCGCTTCCGCGTCAACGCCTTCATGCAGCAGGGCCAGGTCGGCCTGGTGCTGCGCACGATCCC
GCAGACGCTGCCGACGATCGACACGCTGGGCCTGCCGGGCGTGCTCAAGGACATCGCGATGACCAAGCGCGGCCTGGTCA
TCTTCGTCGGCGCCACCGGCTCGGGCAAGAGCACCTCGCTGGCGGCGATGGTCGACTGGCGCAACGAGCACTCCTACGGC
CACATCATCACCGTCGAGGACCCGATCGAGTTCGTGCACCCGCACAAGAACTGCATCGTCACGCAGCGCGAGGTCGGCGT
CGACACCGACGGCTGGGAGCAGGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAGGTCCGCG
ACCGCGAGACGATGGAGCACGCGGTGGCCTTCGCCGAGACCGGTCACCTGTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCATCAACTTCTTCCCCGAGGAGCGCCGCGGCCAGCTGCTGATGGACCTGTCGCTGAACCT
CAAGGCCCTGGTCAGCCAGCGCCTGATGCCGCGCCAGGAAGGCCGCGGGCGCGTCGCGGCCGTCGAGATCATGCTCAACA
CGCCGCTCGTGTCGGACATGATCTTCAAGGGCGAGATCGGCGAGATCAAGGAGCTGATGAAGCGCTCGCGCGAGCTCGGC
ATGCAGACCTTCGACCAGTCGCTGTTCGACCTCTACGAAGCCAACGCGATCACCTACGAGGACGCGCTGCGCAACGCCGA
CTCGGTCAACGACCTGCGCCTGCAGATCAAGCTCAACAGCCAGCGCGCGCGCAGCCGCGACCTCTCCGCCGGCACGGAGA
ACATGCGCGTCATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(99-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.043

92.328

0.601
  pilU Acinetobacter baylyi ADP1

59.218

94.709

0.561
  pilU Vibrio cholerae strain A1552

52.304

97.619

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.884

89.153

0.418
  pilT Pseudomonas aeruginosa PAK

43.917

89.153

0.392
  pilT Acinetobacter nosocomialis M2

43.844

88.095

0.386
  pilT Acinetobacter baumannii D1279779

43.844

88.095

0.386
  pilT Acinetobacter baumannii strain A118

43.844

88.095

0.386
  pilT Acinetobacter baylyi ADP1

43.844

88.095

0.386
  pilT Pseudomonas stutzeri DSM 10701

43.027

89.153

0.384
  pilT Legionella pneumophila strain ERS1305867

43.243

88.095

0.381
  pilT Legionella pneumophila strain Lp02

43.243

88.095

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.082

84.127

0.362
  pilT Vibrio cholerae strain A1552

43.082

84.127

0.362

Multiple sequence alignment