NTDB

Overview

Name	pilT	Type	Machinery gene
Locus tag	HPQ68_RS03535	Genome accession	NZ_CP053748
Coordinates	776707..777750 (+)	Length	347 a.a.
NCBI ID	WP_050409379.1	Uniprot ID	A0A0K1JZF5
Organism	Massilia sp. erpn
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 771707..782750

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HPQ68_RS03505 (HPQ68_03495)	-	771818..772759 (+)	942	WP_255756477.1	hydrogen peroxide-inducible genes activator	-
HPQ68_RS03510 (HPQ68_03500)	ubiA	772833..773687 (+)	855	WP_255758216.1	4-hydroxybenzoate octaprenyltransferase	-
HPQ68_RS03515 (HPQ68_03505)	-	773771..774145 (+)	375	WP_255756478.1	YqjD family protein	-
HPQ68_RS03520 (HPQ68_03510)	-	774171..774965 (-)	795	WP_255756479.1	transporter substrate-binding domain-containing protein	-
HPQ68_RS03525 (HPQ68_03515)	proC	775070..775906 (-)	837	WP_255756480.1	pyrroline-5-carboxylate reductase	-
HPQ68_RS03530 (HPQ68_03520)	-	775903..776631 (-)	729	WP_255756481.1	YggS family pyridoxal phosphate-dependent enzyme	-
HPQ68_RS03535 (HPQ68_03525)	pilT	776707..777750 (+)	1044	WP_050409379.1	type IV pilus twitching motility protein PilT	Machinery gene
HPQ68_RS03540 (HPQ68_03530)	pilU	777778..778914 (+)	1137	WP_255756482.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HPQ68_RS03545 (HPQ68_03535)	-	778914..779399 (+)	486	WP_050409381.1	glutathione peroxidase	-
HPQ68_RS03550 (HPQ68_03540)	-	779578..782040 (+)	2463	WP_255756483.1	bifunctional diguanylate cyclase/phosphodiesterase	-

Sequence

Protein

Download Length: 347 a.a. Molecular weight: 38060.71 Da Isoelectric Point: 6.7269

                                >NTDB_id=446879 HPQ68_RS03535 WP_050409379.1 776707..777750(+) (pilT) [Massilia sp. erpn]

MDISELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPPLEHKDVHGMIYDIMNDGQRKAYEEMLECDFSFAIPGLAR

FRVNAYNQERGASAVLRTIPSKILSLEELNAPRIFAEFALRPRGLVLVTGPTGSGKSTTLAAMINHLNENEYGHILTIED

PIEFVHESKKCLINQREVGPHTLSFNNALRSALREDPDAILVGELRDLETIRLALSAAETGHLVFGTLHTSSAAKTIDRI

VDVFPAEEKEMVRAMLSESLQAVISQTLLKTKDGSGRVAAHEIMVGTPAIRNLIREAKIAQMYSAIQTGSNVGMQTLDQN

LTDLVRRNVISAAAARSAAKIPENFPG

Nucleotide

Download Length: 1044 bp

                                >NTDB_id=446879 HPQ68_RS03535 WP_050409379.1 776707..777750(+) (pilT) [Massilia sp. erpn]

ATGGACATCTCCGAACTTCTCGCATTCTCCGTCAAGAACAAGGCATCGGACCTGCACCTCTCGGCCGGTCTGCCGCCGAT

GATACGGGTGCATGGCGATGTACGGCGCATCAACCTGCCGCCGCTGGAGCACAAGGACGTGCACGGCATGATCTATGACA

TCATGAACGACGGCCAGCGCAAGGCGTATGAGGAGATGCTCGAATGCGACTTCTCCTTCGCCATACCGGGCCTGGCGCGC

TTCCGCGTCAACGCCTACAACCAGGAGCGCGGCGCCTCGGCCGTGCTGCGCACGATCCCGTCCAAGATCCTGTCGCTGGA

AGAACTCAACGCGCCGCGCATCTTCGCCGAATTCGCGCTCAGGCCGCGCGGCCTGGTGCTGGTGACGGGGCCGACCGGTT

CCGGCAAATCGACCACGCTGGCAGCCATGATCAACCACCTCAACGAAAATGAGTATGGCCACATCCTGACCATCGAAGAC

CCGATCGAGTTCGTGCACGAATCGAAGAAATGCCTGATCAACCAGCGCGAAGTGGGGCCGCACACCCTGTCCTTCAACAA

CGCGCTGCGCTCGGCGCTGCGCGAAGATCCGGACGCCATCCTGGTGGGCGAGCTGCGCGACCTGGAAACCATCCGCCTGG

CGCTGTCGGCCGCCGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGTATC

GTCGACGTCTTCCCGGCCGAGGAAAAGGAAATGGTGCGCGCCATGCTGTCCGAATCGCTGCAGGCGGTGATCTCGCAGAC

CCTGCTGAAAACCAAGGACGGCTCGGGCCGCGTGGCGGCGCACGAGATCATGGTCGGCACGCCCGCCATCCGCAACCTGA

TCCGCGAAGCCAAGATCGCGCAGATGTATTCGGCCATCCAGACCGGCAGCAATGTGGGCATGCAGACGCTGGACCAGAAC

CTTACGGACCTGGTGCGGCGCAATGTTATTTCGGCCGCCGCCGCGCGTTCGGCCGCCAAGATTCCGGAAAACTTCCCCGG

CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A0K1JZF5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilT	Acinetobacter nosocomialis M2	77.101	99.424	0.767
pilT	Acinetobacter baumannii D1279779	77.101	99.424	0.767
pilT	Acinetobacter baumannii strain A118	77.101	99.424	0.767
pilT	Pseudomonas aeruginosa PAK	77.101	99.424	0.767
pilT	Pseudomonas stutzeri DSM 10701	75.072	99.424	0.746
pilT	Acinetobacter baylyi ADP1	74.203	99.424	0.738
pilT	Legionella pneumophila strain Lp02	75.516	97.695	0.738
pilT	Legionella pneumophila strain ERS1305867	75.516	97.695	0.738
pilT	Neisseria gonorrhoeae MS11	69.275	99.424	0.689
pilT	Neisseria meningitidis 8013	69.275	99.424	0.689
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	68.546	97.118	0.666
pilT	Vibrio cholerae strain A1552	68.546	97.118	0.666
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	51.594	99.424	0.513
pilU	Acinetobacter baylyi ADP1	42.197	99.712	0.421
pilU	Pseudomonas stutzeri DSM 10701	41.493	96.542	0.401
pilU	Vibrio cholerae strain A1552	41.003	97.695	0.401

Detailed information NTG0446879