Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HPQ68_RS03540 Genome accession   NZ_CP053748
Coordinates   777778..778914 (+) Length   378 a.a.
NCBI ID   WP_255756482.1    Uniprot ID   -
Organism   Massilia sp. erpn     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 772778..783914
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPQ68_RS03510 (HPQ68_03500) ubiA 772833..773687 (+) 855 WP_255758216.1 4-hydroxybenzoate octaprenyltransferase -
  HPQ68_RS03515 (HPQ68_03505) - 773771..774145 (+) 375 WP_255756478.1 YqjD family protein -
  HPQ68_RS03520 (HPQ68_03510) - 774171..774965 (-) 795 WP_255756479.1 transporter substrate-binding domain-containing protein -
  HPQ68_RS03525 (HPQ68_03515) proC 775070..775906 (-) 837 WP_255756480.1 pyrroline-5-carboxylate reductase -
  HPQ68_RS03530 (HPQ68_03520) - 775903..776631 (-) 729 WP_255756481.1 YggS family pyridoxal phosphate-dependent enzyme -
  HPQ68_RS03535 (HPQ68_03525) pilT 776707..777750 (+) 1044 WP_050409379.1 type IV pilus twitching motility protein PilT Machinery gene
  HPQ68_RS03540 (HPQ68_03530) pilU 777778..778914 (+) 1137 WP_255756482.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HPQ68_RS03545 (HPQ68_03535) - 778914..779399 (+) 486 WP_050409381.1 glutathione peroxidase -
  HPQ68_RS03550 (HPQ68_03540) - 779578..782040 (+) 2463 WP_255756483.1 bifunctional diguanylate cyclase/phosphodiesterase -
  HPQ68_RS03555 (HPQ68_03545) glcF 782027..783265 (-) 1239 WP_255756484.1 glycolate oxidase subunit GlcF -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41919.44 Da        Isoelectric Point: 6.7284

>NTDB_id=446880 HPQ68_RS03540 WP_255756482.1 777778..778914(+) (pilU) [Massilia sp. erpn]
MERDQASKFMFDLLRLMVSKKGSDLFITAGFPPAIKIDGKLTPVSNQVLTPAHTADLARSIMNDKQTASFEQTKEANFAI
SPADLGRFRVSAFVQMSSVGMVLRTINSAIPKLEDLGLPEVLKEIIMAKRGLVIMVGATGSGKSTTLAAMVGYRNENSHG
HIITIEDPVEFVHPHKNCIITQREVGVDTEDWEVALKNTLRQAPDVIQIGEIRDRQTMDHAIAFAETGHLCLATLHANSA
NQALDRIINFFPEERRQQLLMDLSLNLKGMISQRLIPLKEAKGRAVAIEIMLNSPLISDLIFKGQVHEIKELMKKSRELG
MQTFDQSLFDLHEADKISYEDALRNADSVNDLRLTIKLEGKAAKTRDLSAGTEHLGII

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=446880 HPQ68_RS03540 WP_255756482.1 777778..778914(+) (pilU) [Massilia sp. erpn]
ATGGAACGCGACCAGGCATCCAAATTCATGTTCGACCTGCTGCGCCTGATGGTCAGCAAAAAAGGCTCGGACCTGTTCAT
CACGGCCGGCTTCCCGCCCGCCATCAAGATCGACGGCAAGCTCACGCCGGTCTCCAACCAGGTGCTGACCCCGGCCCACA
CGGCCGACCTGGCGCGCTCGATCATGAACGACAAGCAGACCGCCAGCTTCGAGCAGACCAAGGAAGCCAACTTCGCCATC
AGCCCCGCCGATCTGGGGCGCTTCCGCGTTTCGGCCTTTGTGCAGATGAGTTCCGTCGGCATGGTGCTGCGCACGATCAA
TAGCGCCATCCCCAAGCTGGAAGACCTGGGCCTGCCCGAGGTGCTGAAGGAGATCATCATGGCCAAGCGTGGCCTGGTGA
TCATGGTGGGCGCCACCGGTTCCGGCAAATCGACCACGCTGGCGGCCATGGTGGGCTACCGCAACGAGAACAGCCACGGC
CACATCATCACCATTGAAGACCCGGTGGAATTCGTCCACCCGCACAAGAACTGCATCATCACCCAGCGCGAAGTCGGCGT
GGATACCGAGGACTGGGAAGTAGCGCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCCAGATCGGCGAGATCCGCG
ACCGCCAGACCATGGACCACGCCATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTCGACCGCATCATCAACTTCTTCCCCGAGGAGCGCCGCCAGCAGCTGCTGATGGACCTGTCGCTGAATCT
GAAAGGCATGATCTCGCAGCGCCTGATTCCGCTCAAGGAAGCCAAGGGGCGCGCCGTCGCCATCGAGATCATGCTCAATT
CGCCGCTGATCTCGGACCTGATCTTCAAGGGCCAGGTGCATGAGATCAAGGAGCTGATGAAGAAATCGCGCGAATTGGGC
ATGCAGACTTTCGACCAGTCCCTGTTCGACCTGCACGAAGCCGACAAGATCAGCTACGAGGACGCGCTGCGCAACGCCGA
CTCGGTCAACGACCTGCGCCTGACCATCAAGCTGGAAGGCAAGGCCGCCAAGACGCGCGACCTGTCGGCCGGCACCGAAC
ACCTGGGGATCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(84-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.162

91.534

0.587
  pilU Acinetobacter baylyi ADP1

59.384

94.444

0.561
  pilU Vibrio cholerae strain A1552

58.944

90.212

0.532
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.985

88.624

0.381
  pilT Pseudomonas aeruginosa PAK

42.687

88.624

0.378
  pilT Acinetobacter nosocomialis M2

43.202

87.566

0.378
  pilT Legionella pneumophila strain ERS1305867

42.598

87.566

0.373
  pilT Legionella pneumophila strain Lp02

42.598

87.566

0.373
  pilT Acinetobacter baylyi ADP1

43.75

84.656

0.37
  pilT Pseudomonas stutzeri DSM 10701

41.791

88.624

0.37
  pilT Acinetobacter baumannii D1279779

43.849

83.862

0.368
  pilT Acinetobacter baumannii strain A118

43.849

83.862

0.368