Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HMY34_RS10925 Genome accession   NZ_CP053482
Coordinates   2223151..2224185 (+) Length   344 a.a.
NCBI ID   WP_202715528.1    Uniprot ID   -
Organism   Thiothrix subterranea strain Ku-5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2218151..2229185
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HMY34_RS10905 (HMY34_10890) - 2218535..2218936 (+) 402 WP_202715524.1 DUF3037 domain-containing protein -
  HMY34_RS10910 (HMY34_10895) - 2219010..2221835 (+) 2826 WP_202715525.1 TIR domain-containing protein -
  HMY34_RS10915 (HMY34_10900) - 2221905..2222402 (+) 498 WP_202715526.1 DUF4124 domain-containing protein -
  HMY34_RS10920 (HMY34_10905) - 2222403..2223089 (-) 687 WP_202715527.1 YggS family pyridoxal phosphate-dependent enzyme -
  HMY34_RS10925 (HMY34_10910) pilT 2223151..2224185 (+) 1035 WP_202715528.1 type IV pilus twitching motility protein PilT Machinery gene
  HMY34_RS10930 (HMY34_10915) pilU 2224252..2225409 (+) 1158 WP_202715529.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HMY34_RS10935 (HMY34_10920) - 2225406..2226536 (+) 1131 WP_202715530.1 PilT/PilU family type 4a pilus ATPase -
  HMY34_RS10940 (HMY34_10925) bioD 2226533..2227192 (-) 660 WP_202715531.1 dethiobiotin synthase -
  HMY34_RS10945 (HMY34_10930) - 2227478..2227996 (+) 519 WP_202715532.1 DUF1566 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38186.73 Da        Isoelectric Point: 8.1677

>NTDB_id=444998 HMY34_RS10925 WP_202715528.1 2223151..2224185(+) (pilT) [Thiothrix subterranea strain Ku-5]
MDITQLLAFSVKNNASDLHLSGGMPPMIRVDGEMRKINLPVLDHKQVHALVYDIMNDHQRKNYEEHWECDFSFEIPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTLDQLGAPGIFRKISENPRGLVLVTGPTGSGKSTTLAAMVDYKNDTDYGHILTIED
PIEFVHESKKSLVNQREVHRDTKGFEAALRSALREDPDTILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAPKTIDRI
VDVFPAAEKEMVRSMLSESLQAVISQTLLKKKGGGRVAAHEILIGNRAVRNLIRENKIAQMRSAMQTGQSDGMQTLDQSL
KNLVMKGLVDREAARRKADNPDSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=444998 HMY34_RS10925 WP_202715528.1 2223151..2224185(+) (pilT) [Thiothrix subterranea strain Ku-5]
ATGGACATTACCCAACTGCTGGCGTTCAGCGTCAAAAACAATGCCTCCGACTTGCACTTGTCGGGCGGGATGCCCCCCAT
GATCCGTGTCGATGGCGAAATGCGCAAGATCAACTTGCCGGTACTCGACCATAAACAGGTACACGCGCTGGTGTACGACA
TCATGAATGATCACCAACGCAAAAATTACGAAGAGCATTGGGAATGCGATTTCTCGTTTGAAATTCCCGGTGTGGCGCGG
TTTCGTGTGAATGCCTTTAACCAAAACCGTGGTGCTGGTGCGGTGTTTCGTACCATCCCCAGCAAAGTATTAACACTCGA
TCAGTTGGGTGCGCCCGGTATTTTTCGGAAAATTTCGGAAAACCCACGCGGCTTGGTGTTGGTAACAGGGCCGACCGGTT
CGGGCAAATCCACCACGCTTGCGGCAATGGTCGATTACAAAAATGACACCGATTACGGGCATATTCTTACCATCGAAGAC
CCGATCGAATTCGTGCACGAAAGCAAAAAAAGTCTGGTCAACCAACGTGAGGTGCATCGCGACACCAAAGGCTTTGAAGC
CGCTCTCCGTTCCGCTTTGCGTGAAGACCCGGATACCATTCTGGTCGGGGAAATGCGTGACTTGGAAACCATTCGCTTGG
CATTGACGGCGGCGGAAACCGGGCATTTGGTGTTTGGCACGTTGCATACGACATCCGCACCCAAAACCATTGACCGTATT
GTGGATGTGTTCCCCGCTGCCGAAAAAGAGATGGTGCGTTCCATGCTTTCCGAATCCTTGCAGGCGGTGATTTCGCAAAC
CCTGCTGAAGAAAAAAGGCGGCGGGCGCGTGGCAGCACACGAAATTCTGATTGGCAATCGGGCGGTGCGTAACCTGATTC
GCGAAAATAAGATTGCGCAAATGCGTTCTGCAATGCAAACCGGGCAAAGTGACGGGATGCAAACCCTTGATCAATCACTG
AAAAACTTGGTGATGAAAGGTTTGGTGGATCGTGAAGCGGCGCGGCGCAAAGCCGATAACCCGGATTCTATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

79.36

100

0.794
  pilT Pseudomonas aeruginosa PAK

79.36

100

0.794
  pilT Acinetobacter baumannii D1279779

77.907

100

0.779
  pilT Acinetobacter baumannii strain A118

77.907

100

0.779
  pilT Acinetobacter nosocomialis M2

77.907

100

0.779
  pilT Acinetobacter baylyi ADP1

76.453

100

0.765
  pilT Neisseria gonorrhoeae MS11

71.594

100

0.718
  pilT Neisseria meningitidis 8013

71.594

100

0.718
  pilT Legionella pneumophila strain Lp02

71.765

98.837

0.709
  pilT Legionella pneumophila strain ERS1305867

71.765

98.837

0.709
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.257

98.547

0.663
  pilT Vibrio cholerae strain A1552

67.257

98.547

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

40.87

100

0.41
  pilU Pseudomonas stutzeri DSM 10701

40.29

100

0.404
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395