Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HMY34_RS10930 Genome accession   NZ_CP053482
Coordinates   2224252..2225409 (+) Length   385 a.a.
NCBI ID   WP_202715529.1    Uniprot ID   -
Organism   Thiothrix subterranea strain Ku-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2219252..2230409
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HMY34_RS10915 (HMY34_10900) - 2221905..2222402 (+) 498 WP_202715526.1 DUF4124 domain-containing protein -
  HMY34_RS10920 (HMY34_10905) - 2222403..2223089 (-) 687 WP_202715527.1 YggS family pyridoxal phosphate-dependent enzyme -
  HMY34_RS10925 (HMY34_10910) pilT 2223151..2224185 (+) 1035 WP_202715528.1 type IV pilus twitching motility protein PilT Machinery gene
  HMY34_RS10930 (HMY34_10915) pilU 2224252..2225409 (+) 1158 WP_202715529.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HMY34_RS10935 (HMY34_10920) - 2225406..2226536 (+) 1131 WP_202715530.1 PilT/PilU family type 4a pilus ATPase -
  HMY34_RS10940 (HMY34_10925) bioD 2226533..2227192 (-) 660 WP_202715531.1 dethiobiotin synthase -
  HMY34_RS10945 (HMY34_10930) - 2227478..2227996 (+) 519 WP_202715532.1 DUF1566 domain-containing protein -
  HMY34_RS10950 (HMY34_10935) - 2227999..2229492 (-) 1494 WP_202715533.1 NuoM family protein -

Sequence

Protein


Download         Length: 385 a.a.        Molecular weight: 43036.69 Da        Isoelectric Point: 7.5373

>NTDB_id=444999 HMY34_RS10930 WP_202715529.1 2224252..2225409(+) (pilU) [Thiothrix subterranea strain Ku-5]
MDRDAAVSYVHKLLATMLEKKASDLYVTAEAAPSAKIASEMVPLTNQPLNEQTARMLVRAIMNDRQLKQFEEDMECNFSI
SLPGKARFRVNAFTQRGCAGMVLRHIPNHIPGLKELGLPPVLREIAMTKRGLVIMVGATGSGKSTTLASMVDFRNTNSKG
HIVTIEDPIEFVHQHKGCLITQREVGVDTSSYEIAMKNTLRQAPDVILLGEIRDREAMDYAIAYAETGHLCLSTLHANSS
NQAIDRIINFFPEERRNQLLMDLSLNLKAVVSQRLVRKAKGEGRLPAVEVVINTPLMADLILKGDVPGMKILTSKSREHG
MRTFDQALFDLIEARQITVHEALRHADSQNDLRLRLKLESRFAQDNDFFKGLDELAIEPLENATR

Nucleotide


Download         Length: 1158 bp        

>NTDB_id=444999 HMY34_RS10930 WP_202715529.1 2224252..2225409(+) (pilU) [Thiothrix subterranea strain Ku-5]
ATGGATCGCGATGCTGCTGTCAGCTATGTGCATAAGTTATTGGCTACTATGTTGGAAAAGAAAGCTTCCGACTTGTATGT
CACCGCCGAGGCTGCCCCTTCTGCCAAAATTGCCAGCGAAATGGTGCCGTTAACCAATCAGCCATTGAATGAGCAAACGG
CGCGGATGTTGGTGCGGGCGATTATGAACGACCGCCAGCTCAAACAGTTTGAGGAGGACATGGAATGCAACTTTTCCATT
AGTCTGCCGGGCAAGGCGCGATTCCGGGTGAATGCGTTCACGCAACGCGGTTGTGCGGGGATGGTGTTACGCCATATTCC
TAACCATATTCCCGGTTTGAAAGAGTTGGGGTTGCCGCCGGTATTGCGCGAGATTGCCATGACCAAACGCGGTTTGGTGA
TTATGGTGGGGGCGACGGGTTCGGGTAAATCGACCACACTGGCGTCGATGGTCGATTTTCGCAATACCAATTCCAAAGGG
CATATCGTCACGATTGAAGACCCGATTGAGTTTGTGCATCAGCACAAGGGGTGCTTGATTACGCAGCGCGAAGTGGGGGT
CGATACCAGCAGTTATGAGATTGCGATGAAAAATACCTTGCGCCAAGCCCCTGATGTGATTTTGTTAGGCGAAATTCGGG
ATCGTGAGGCGATGGATTACGCGATTGCCTACGCTGAAACCGGGCATTTGTGCTTGTCGACCTTGCACGCCAACAGTTCC
AACCAAGCGATCGACCGTATTATCAACTTTTTCCCCGAAGAACGCCGCAACCAGTTATTGATGGATTTATCCCTCAACCT
GAAGGCGGTGGTATCACAGCGCTTGGTGCGTAAAGCCAAAGGGGAAGGGCGTTTGCCAGCGGTGGAAGTGGTGATTAATA
CGCCATTAATGGCAGATTTAATTCTGAAAGGCGATGTGCCGGGCATGAAGATTCTGACCTCGAAATCGCGCGAACACGGG
ATGCGCACCTTTGACCAAGCGCTATTTGATTTGATTGAAGCGCGGCAAATCACCGTGCATGAAGCGTTGCGTCATGCCGA
TTCGCAGAATGACTTGCGCTTACGCCTCAAATTGGAAAGCCGGTTTGCGCAAGATAATGACTTCTTTAAGGGGTTGGATG
AGTTGGCGATTGAGCCGCTGGAGAATGCCACACGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.026

90.649

0.535
  pilU Acinetobacter baylyi ADP1

56.484

90.13

0.509
  pilU Vibrio cholerae strain A1552

51.149

90.39

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.807

87.532

0.392
  pilT Acinetobacter baumannii D1279779

42.941

88.312

0.379
  pilT Acinetobacter baumannii strain A118

42.941

88.312

0.379
  pilT Acinetobacter nosocomialis M2

42.647

88.312

0.377
  pilT Vibrio cholerae strain A1552

41.261

90.649

0.374
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.261

90.649

0.374
  pilT Legionella pneumophila strain Lp02

43.243

86.494

0.374
  pilT Legionella pneumophila strain ERS1305867

43.243

86.494

0.374
  pilT Acinetobacter baylyi ADP1

43.963

83.896

0.369
  pilT Pseudomonas aeruginosa PAK

43.034

83.896

0.361