Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DSM104443_RS00580 Genome accession   NZ_CP053069
Coordinates   126171..127214 (-) Length   347 a.a.
NCBI ID   WP_171088780.1    Uniprot ID   -
Organism   Usitatibacter rugosus strain 0125_3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 121171..132214
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DSM104443_RS00560 (DSM104443_00112) - 122655..123533 (+) 879 WP_171088777.1 EAL domain-containing protein -
  DSM104443_RS00565 (DSM104443_00113) - 123591..124142 (+) 552 WP_212756856.1 ferritin-like domain-containing protein -
  DSM104443_RS00570 (DSM104443_00114) - 124243..124890 (+) 648 WP_171088778.1 Crp/Fnr family transcriptional regulator -
  DSM104443_RS00575 (DSM104443_00115) pilU 125003..126142 (-) 1140 WP_171088779.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DSM104443_RS00580 (DSM104443_00116) pilT 126171..127214 (-) 1044 WP_171088780.1 type IV pilus twitching motility protein PilT Machinery gene
  DSM104443_RS00585 (DSM104443_00117) - 127258..127947 (+) 690 WP_171088781.1 YggS family pyridoxal phosphate-dependent enzyme -
  DSM104443_RS00590 (DSM104443_00118) proC 127944..128756 (+) 813 WP_171088782.1 pyrroline-5-carboxylate reductase -
  DSM104443_RS00595 (DSM104443_00119) - 128762..129355 (+) 594 WP_171088783.1 YggT family protein -
  DSM104443_RS00600 (DSM104443_00120) - 129502..129756 (+) 255 WP_171088784.1 hypothetical protein -
  DSM104443_RS00605 - 129743..129934 (+) 192 WP_171088785.1 hypothetical protein -
  DSM104443_RS00610 (DSM104443_00121) - 130029..130232 (+) 204 WP_171088786.1 hypothetical protein -
  DSM104443_RS00615 (DSM104443_00122) - 130235..131905 (-) 1671 WP_212756858.1 long-chain fatty acid--CoA ligase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38128.88 Da        Isoelectric Point: 6.8357

>NTDB_id=442660 DSM104443_RS00580 WP_171088780.1 126171..127214(-) (pilT) [Usitatibacter rugosus strain 0125_3]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVPPLEHKDVHGMVYDIMNDSQRKVYEETLEVDFSFEIPNLAR
FRVNAFNQNRGAGAVFRTIPSKVLTLEDLNAPKIFKEIANQPRGLVLVTGPTGSGKSTTLAAMVNDVNETEYGHILTVED
PIEFVHEAKKCLINQREVGPHTLSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPAAEKEMIRAMLSESLRAVISQTLCKTKDGTGRVAAHEIMIGTPAIRNLIREAKVAQMYSAIQTGGNIGMQTLDQN
LQDLVKRNLISVPEAKTKAANKDAFIG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=442660 DSM104443_RS00580 WP_171088780.1 126171..127214(-) (pilT) [Usitatibacter rugosus strain 0125_3]
ATGGATATCGCAGAGCTTCTCGCCTTTTCGGTCAAGAACAAGGCTTCCGACCTTCACCTCTCCGCCGGCCTGCCGCCGAT
GATCCGCGTGCACGGCGACGTGCGGCGCATCAACGTGCCCCCGCTCGAGCACAAGGACGTGCACGGGATGGTGTACGACA
TCATGAACGACTCGCAGCGCAAGGTGTACGAGGAAACGCTGGAGGTCGACTTCAGCTTCGAGATCCCCAACCTCGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGTGCTGTGTTCCGGACCATTCCGTCGAAGGTCCTCACGCTCGA
GGACCTGAACGCCCCCAAGATCTTCAAGGAAATCGCCAACCAGCCGCGCGGCCTGGTGCTGGTGACGGGCCCGACGGGCT
CGGGCAAGTCCACGACGCTGGCCGCCATGGTCAACGACGTGAACGAGACCGAATACGGCCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTGCACGAGGCGAAGAAGTGCCTCATCAACCAGCGCGAGGTCGGACCGCACACGCTGTCGTTCTCCAA
CGCGCTTCGCAGCGCCCTTCGCGAGGACCCGGACATCATCCTCGTGGGCGAGATGCGAGATCTGGAAACCATCCGCCTCG
CCATGACGGCCGCCGAAACGGGCCACCTGGTGTTCGGCACGCTGCACACGTCCTCGGCCGCCAAGACCGTGGACCGCATC
ATCGACGTCTTCCCGGCGGCGGAAAAGGAAATGATCCGAGCGATGCTGTCGGAATCGCTGCGCGCGGTGATCTCGCAAAC
GCTCTGCAAGACCAAGGACGGCACGGGTCGCGTCGCGGCGCACGAGATCATGATCGGCACGCCCGCGATCCGGAACCTCA
TTCGCGAGGCGAAGGTCGCGCAGATGTACTCCGCGATCCAGACCGGCGGCAACATCGGGATGCAGACGCTCGACCAGAAC
CTGCAGGACCTCGTGAAGCGCAACCTCATCTCGGTCCCCGAGGCCAAGACGAAGGCCGCCAACAAGGATGCGTTCATCGG
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.035

99.712

0.778
  pilT Acinetobacter baumannii D1279779

78.035

99.712

0.778
  pilT Acinetobacter baumannii strain A118

78.035

99.712

0.778
  pilT Pseudomonas aeruginosa PAK

76.812

99.424

0.764
  pilT Pseudomonas stutzeri DSM 10701

75.942

99.424

0.755
  pilT Acinetobacter baylyi ADP1

75.362

99.424

0.749
  pilT Legionella pneumophila strain Lp02

73.913

99.424

0.735
  pilT Legionella pneumophila strain ERS1305867

73.913

99.424

0.735
  pilT Neisseria meningitidis 8013

69.855

99.424

0.695
  pilT Neisseria gonorrhoeae MS11

69.565

99.424

0.692
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.118

97.406

0.683
  pilT Vibrio cholerae strain A1552

70.118

97.406

0.683
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

99.424

0.513
  pilU Acinetobacter baylyi ADP1

42.262

96.83

0.409
  pilU Vibrio cholerae strain A1552

41.791

96.542

0.403
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.542

0.401