Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DSM104443_RS00575 Genome accession   NZ_CP053069
Coordinates   125003..126142 (-) Length   379 a.a.
NCBI ID   WP_171088779.1    Uniprot ID   A0A6M4GP11
Organism   Usitatibacter rugosus strain 0125_3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 120003..131142
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DSM104443_RS00555 (DSM104443_00111) - 120055..122541 (+) 2487 WP_171088776.1 ATP-binding protein -
  DSM104443_RS00560 (DSM104443_00112) - 122655..123533 (+) 879 WP_171088777.1 EAL domain-containing protein -
  DSM104443_RS00565 (DSM104443_00113) - 123591..124142 (+) 552 WP_212756856.1 ferritin-like domain-containing protein -
  DSM104443_RS00570 (DSM104443_00114) - 124243..124890 (+) 648 WP_171088778.1 Crp/Fnr family transcriptional regulator -
  DSM104443_RS00575 (DSM104443_00115) pilU 125003..126142 (-) 1140 WP_171088779.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DSM104443_RS00580 (DSM104443_00116) pilT 126171..127214 (-) 1044 WP_171088780.1 type IV pilus twitching motility protein PilT Machinery gene
  DSM104443_RS00585 (DSM104443_00117) - 127258..127947 (+) 690 WP_171088781.1 YggS family pyridoxal phosphate-dependent enzyme -
  DSM104443_RS00590 (DSM104443_00118) proC 127944..128756 (+) 813 WP_171088782.1 pyrroline-5-carboxylate reductase -
  DSM104443_RS00595 (DSM104443_00119) - 128762..129355 (+) 594 WP_171088783.1 YggT family protein -
  DSM104443_RS00600 (DSM104443_00120) - 129502..129756 (+) 255 WP_171088784.1 hypothetical protein -
  DSM104443_RS00605 - 129743..129934 (+) 192 WP_171088785.1 hypothetical protein -
  DSM104443_RS00610 (DSM104443_00121) - 130029..130232 (+) 204 WP_171088786.1 hypothetical protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42223.67 Da        Isoelectric Point: 6.8821

>NTDB_id=442659 DSM104443_RS00575 WP_171088779.1 125003..126142(-) (pilU) [Usitatibacter rugosus strain 0125_3]
MEREQSLKFMHDLLRALIARRGSDLFITAGFPPAMKVDGKVTPVSQQSLSPVHSQELCRSIMTDKQAAEFEATNECNFAI
SPSGIGRFRVNAFVQQGRVGIVLRTITTTIPKFDDLGLPHVLKDVAMTKRGLVIFVGGTGSGKSTSLAAMIGHRNENSYG
HIITIEDPVEYVHEHNNCIITQREVGVDTDNWFAALKNTLRQAPDVILIGEIRERETMEFAIAFAETGHLCMSTLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLKAIISQRLIPKKATKGRVAAIEILLNSPLMSDLIFKGEVHEMKALVARSRELG
MQTFDQALFDLFEADMITFEDALRNADSVNDLRLKIKLESKNAKNRDLGSGLEHLEIVK

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=442659 DSM104443_RS00575 WP_171088779.1 125003..126142(-) (pilU) [Usitatibacter rugosus strain 0125_3]
ATGGAACGCGAACAGTCACTCAAGTTCATGCACGACCTGCTGCGCGCCCTGATCGCGCGCCGTGGCTCCGATCTCTTCAT
CACCGCGGGCTTTCCCCCGGCGATGAAGGTGGACGGCAAGGTGACGCCCGTGTCCCAGCAGAGCCTGTCGCCGGTGCACT
CGCAGGAGCTGTGCCGCTCGATCATGACGGACAAGCAGGCGGCCGAATTCGAAGCCACCAACGAGTGCAACTTCGCGATC
TCGCCCTCGGGCATCGGCCGCTTCCGCGTGAATGCGTTCGTGCAGCAGGGCCGCGTCGGCATCGTGCTTCGCACCATCAC
CACGACCATCCCCAAGTTCGACGACCTGGGCCTGCCCCACGTGCTGAAGGACGTGGCGATGACCAAGCGCGGCCTGGTGA
TCTTCGTGGGCGGCACGGGCTCGGGCAAGTCGACCTCGCTCGCCGCGATGATCGGCCACCGCAACGAGAACAGCTACGGC
CACATCATCACCATCGAGGACCCGGTCGAATACGTCCACGAGCACAACAACTGCATCATCACGCAGCGCGAAGTGGGCGT
GGACACCGACAACTGGTTCGCCGCGCTCAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTCATCGGCGAGATCCGCG
AGAGGGAGACGATGGAGTTCGCCATCGCCTTCGCGGAAACCGGCCACCTGTGCATGTCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTCCCCGAAGAGCGCCGCGCCCAGCTGCTGATGGACTTGTCGCTGAACTT
GAAGGCCATCATCTCGCAGCGCCTGATCCCCAAGAAGGCCACCAAGGGCCGCGTGGCCGCGATCGAGATCCTGCTCAACT
CGCCGCTCATGTCGGACCTCATCTTCAAGGGCGAGGTGCACGAGATGAAGGCACTCGTGGCCCGCAGCCGCGAGCTCGGC
ATGCAGACCTTCGACCAGGCGCTCTTCGACCTCTTCGAGGCCGACATGATCACCTTCGAAGACGCGCTGCGGAATGCGGA
CTCGGTCAACGACCTTCGCCTGAAGATCAAGCTCGAGAGCAAGAACGCGAAGAACCGCGACCTCGGCTCCGGCCTGGAGC
ACCTCGAGATCGTGAAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M4GP11

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.043

92.084

0.599
  pilU Acinetobacter baylyi ADP1

59.016

96.57

0.57
  pilU Vibrio cholerae strain A1552

54.696

95.515

0.522
  pilT Pseudomonas aeruginosa PAK

43.713

88.127

0.385
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.582

88.391

0.385
  pilT Pseudomonas stutzeri DSM 10701

42.216

88.127

0.372
  pilT Acinetobacter nosocomialis M2

42.296

87.335

0.369
  pilT Acinetobacter baumannii strain A118

42.296

87.335

0.369
  pilT Acinetobacter baumannii D1279779

42.296

87.335

0.369
  pilT Acinetobacter baylyi ADP1

41.994

87.335

0.367
  pilT Legionella pneumophila strain ERS1305867

41.515

87.071

0.361
  pilT Legionella pneumophila strain Lp02

41.515

87.071

0.361