Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HIR79_RS00020 Genome accession   NZ_CP053032
Coordinates   3869..4903 (-) Length   344 a.a.
NCBI ID   WP_119022735.1    Uniprot ID   A0A3B7L9D1
Organism   Halomonas sp. PGE1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1..9903
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HIR79_RS00005 (HIR79_00005) tyrS 182..1381 (-) 1200 WP_169955847.1 tyrosine--tRNA ligase -
  HIR79_RS00010 (HIR79_00010) - 1497..2636 (+) 1140 WP_169955849.1 anhydro-N-acetylmuramic acid kinase -
  HIR79_RS00015 (HIR79_00015) pilU 2677..3819 (-) 1143 WP_119022734.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HIR79_RS00020 (HIR79_00020) pilT 3869..4903 (-) 1035 WP_119022735.1 type IV pilus twitching motility protein PilT Machinery gene
  HIR79_RS00025 (HIR79_00025) - 4970..5689 (+) 720 WP_119022736.1 YggS family pyridoxal phosphate-dependent enzyme -
  HIR79_RS00030 (HIR79_00030) proC 5760..6584 (+) 825 WP_169955851.1 pyrroline-5-carboxylate reductase -
  HIR79_RS00035 (HIR79_00035) - 6665..7246 (+) 582 WP_169955853.1 YggT family protein -
  HIR79_RS00040 (HIR79_00040) - 7293..8450 (+) 1158 WP_110070044.1 homoserine O-acetyltransferase -
  HIR79_RS00045 (HIR79_00045) metW 8450..9049 (+) 600 WP_110070055.1 methionine biosynthesis protein MetW -
  HIR79_RS00050 (HIR79_00050) - 9076..9531 (+) 456 WP_169955855.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37934.53 Da        Isoelectric Point: 6.5089

>NTDB_id=442311 HIR79_RS00020 WP_119022735.1 3869..4903(-) (pilT) [Halomonas sp. PGE1]
MDITELLAFSAKQKASDLHLSAGLPPMIRVDGDIRRLNVPAMEDREVRKLIYDIMADRQRRDYEEFFETDFSFEVPGVSR
FRVNVFNQARGAGAVFRTIPSEVLTLEDLGLGTVFRQLSMLPRGLVLVTGPTGSGKSTTLAAMIDYINDNRYEHILTIED
PVEFVHRSKRCLINQREVHRDTHGFAPALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPGDEKSMVRSMLSESLQAVVSQTLLKRMGGGRVAAHEILIATAAVRNLIREDKVAQIYSAIQTGGNLGMQTLDASL
AKLVADNVVARDEAQAKAKGVLAN

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=442311 HIR79_RS00020 WP_119022735.1 3869..4903(-) (pilT) [Halomonas sp. PGE1]
ATGGATATTACCGAACTGCTGGCGTTCTCGGCAAAACAGAAGGCCTCCGACCTGCATCTTTCGGCGGGCCTGCCGCCGAT
GATCCGGGTCGATGGCGACATTCGCCGCCTGAACGTGCCAGCCATGGAGGACCGCGAGGTCCGCAAGCTGATCTACGACA
TCATGGCCGACCGCCAGCGCCGTGACTACGAGGAGTTCTTCGAGACCGACTTCTCCTTCGAGGTGCCCGGCGTGTCGCGC
TTCCGTGTCAACGTCTTCAACCAGGCGCGTGGCGCGGGCGCGGTGTTCCGTACCATTCCCTCCGAGGTGCTGACCCTGGA
GGACCTCGGCCTCGGCACCGTCTTCCGTCAGCTCTCCATGCTGCCCCGCGGCCTGGTGCTGGTGACCGGCCCCACCGGCT
CGGGCAAGAGCACCACGCTTGCGGCGATGATCGACTACATCAACGATAACCGCTACGAGCACATCCTCACCATCGAGGAC
CCGGTGGAATTCGTTCACCGCAGCAAGCGCTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACCCACGGCTTCGCCCC
GGCGCTGCGCAGCGCCCTGCGCGAGGACCCCGACGTGATCCTGGTGGGTGAGCTGCGTGACCTGGAGACCATTCGCCTGG
CGCTGACCGCGGCGGAGACCGGTCACCTGGTGTTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGACCGGATC
ATCGACGTCTTCCCCGGTGATGAGAAATCCATGGTGCGCTCGATGCTCTCGGAGTCGCTGCAGGCGGTGGTCTCCCAGAC
CCTGCTCAAGCGCATGGGCGGCGGGCGCGTCGCGGCCCACGAGATCCTGATCGCCACCGCGGCGGTGCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATCTACTCGGCCATCCAGACCGGCGGCAACCTGGGCATGCAGACCCTCGATGCCTCGCTG
GCCAAGCTGGTGGCGGACAACGTGGTGGCGCGCGACGAGGCGCAGGCCAAGGCCAAGGGCGTGCTGGCCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3B7L9D1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

79.351

98.547

0.782
  pilT Pseudomonas aeruginosa PAK

78.466

98.547

0.773
  pilT Acinetobacter baumannii D1279779

77.581

98.547

0.765
  pilT Acinetobacter baumannii strain A118

77.581

98.547

0.765
  pilT Acinetobacter nosocomialis M2

77.286

98.547

0.762
  pilT Acinetobacter baylyi ADP1

76.991

98.547

0.759
  pilT Legionella pneumophila strain Lp02

72.485

98.256

0.712
  pilT Legionella pneumophila strain ERS1305867

72.485

98.256

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria meningitidis 8013

66.471

98.837

0.657
  pilT Neisseria gonorrhoeae MS11

66.176

98.837

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.802

98.547

0.52
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Acinetobacter baylyi ADP1

40.923

94.477

0.387
  pilU Vibrio cholerae strain A1552

39.701

97.384

0.387