Detailed information NTG0442310

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HIR79_RS00015
• Genome accession: NZ_CP053032
• Coordinates: 2677..3819 (-)
• Length: 380 a.a.
• NCBI ID: WP_119022734.1
• Uniprot ID: A0A3B7L907
• Organism: Halomonas sp. PGE1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1..8819

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HIR79_RS00005 (HIR79_00005)	tyrS	182..1381 (-)	1200	WP_169955847.1	tyrosine--tRNA ligase	-
HIR79_RS00010 (HIR79_00010)	-	1497..2636 (+)	1140	WP_169955849.1	anhydro-N-acetylmuramic acid kinase	-
HIR79_RS00015 (HIR79_00015)	pilU	2677..3819 (-)	1143	WP_119022734.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HIR79_RS00020 (HIR79_00020)	pilT	3869..4903 (-)	1035	WP_119022735.1	type IV pilus twitching motility protein PilT	Machinery gene
HIR79_RS00025 (HIR79_00025)	-	4970..5689 (+)	720	WP_119022736.1	YggS family pyridoxal phosphate-dependent enzyme	-
HIR79_RS00030 (HIR79_00030)	proC	5760..6584 (+)	825	WP_169955851.1	pyrroline-5-carboxylate reductase	-
HIR79_RS00035 (HIR79_00035)	-	6665..7246 (+)	582	WP_169955853.1	YggT family protein	-
HIR79_RS00040 (HIR79_00040)	-	7293..8450 (+)	1158	WP_110070044.1	homoserine O-acetyltransferase	-

Sequence

Protein

• Length: 380 a.a.
• Molecular weight: 42197.49 Da
• Isoelectric Point: 6.1385

>NTDB_id=442310 HIR79_RS00015 WP_119022734.1 2677..3819(-) (pilU) [Halomonas sp. PGE1]
MTAKEWLYQLLDIMVQKEASDLLISVNAPPTLKMAGKLTPLGDQGLSVAQVDELVSASIPEAVMARFQVEREANFALSLR
DKGRFRVSAFQQRNQKAMVIRRIAFEIPHLEELSLPPVLGELANNKRGLVFVVGGTGTGKSTTLASMIQQRNETLGGHII
SVEDPIEYVHPHKKAIINQREVGIDTDSFEVALKNTLRQAPDVILIGEIRTRETMEHALTFAETGHLCLATLHANNANQA
LDRIIHFFPHERHEQIWLDLSLNLRAIVAQQLLPTVDGGRCPAIEIMLKSPLIGDLVRKGEVNEIKNVMSRSRDLGMQTF
DQALYDLFRAGKITEEVALVHADSANDLRMMIKYGDAGSEGLAQAREAASQLSLKRDEDF

Nucleotide

• Length: 1143 bp

>NTDB_id=442310 HIR79_RS00015 WP_119022734.1 2677..3819(-) (pilU) [Halomonas sp. PGE1]
ATGACGGCGAAAGAGTGGCTCTACCAGCTGCTGGATATCATGGTGCAGAAGGAGGCGTCGGATCTGCTGATCTCGGTGAA
TGCACCGCCGACCCTCAAGATGGCCGGCAAGCTGACTCCCCTGGGCGACCAGGGGCTCTCCGTGGCCCAGGTCGACGAGC
TGGTCAGCGCGTCGATCCCGGAGGCGGTGATGGCGCGCTTCCAGGTCGAGCGCGAGGCCAACTTCGCGCTCAGCCTCAGG
GACAAGGGGCGCTTTCGCGTCAGCGCCTTCCAGCAGCGCAACCAGAAGGCGATGGTGATCCGGCGCATCGCCTTCGAGAT
CCCCCACCTGGAGGAGCTGTCGCTGCCGCCGGTGCTGGGCGAGCTGGCCAACAACAAGCGTGGCCTGGTGTTCGTGGTGG
GCGGCACCGGTACCGGCAAGTCGACCACCCTGGCCTCGATGATCCAGCAGCGCAACGAGACCCTGGGGGGCCATATCATC
AGCGTCGAGGATCCCATCGAGTACGTGCATCCGCACAAGAAGGCGATCATCAACCAGCGCGAGGTGGGCATCGACACCGA
CTCCTTCGAGGTCGCGCTGAAGAACACCCTGCGCCAGGCGCCCGACGTGATCCTGATCGGCGAGATCCGCACCCGGGAGA
CCATGGAGCATGCGCTGACCTTCGCCGAGACCGGCCACCTCTGCCTGGCGACCCTGCACGCCAACAACGCCAACCAGGCG
CTGGATCGCATCATCCACTTCTTCCCCCACGAGCGGCACGAGCAGATCTGGCTCGACCTCTCGCTCAACCTGCGCGCCAT
CGTGGCCCAGCAGCTGCTGCCCACGGTGGATGGTGGCCGCTGCCCGGCCATCGAGATCATGCTCAAGTCACCGCTGATCG
GCGATCTGGTACGCAAGGGCGAGGTCAACGAGATCAAGAACGTGATGTCGCGCTCCCGAGACCTGGGGATGCAGACCTTC
GACCAGGCGCTCTACGACCTCTTCCGGGCGGGCAAGATCACCGAGGAGGTGGCGCTGGTGCACGCCGACTCGGCCAACGA
CCTGCGCATGATGATCAAGTACGGTGACGCCGGCAGCGAAGGGCTGGCCCAGGCACGCGAGGCGGCAAGCCAGCTCTCGC
TCAAGCGCGACGAGGACTTCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (66-273)

3D structure

• AlphaFold DB: A0A3B7L907

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	60.858	98.158	0.597
pilU	Vibrio cholerae strain A1552	60.345	91.579	0.553
pilU	Acinetobacter baylyi ADP1	54.891	96.842	0.532
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.416	100	0.434
pilT	Neisseria meningitidis 8013	39.942	90.263	0.361