Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IR210_RS04570 Genome accession   NZ_CP063983
Coordinates   944570..945550 (+) Length   326 a.a.
NCBI ID   WP_001443100.1    Uniprot ID   -
Organism   Escherichia coli strain 25DN     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 939570..950550
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IR210_RS04540 (IR210_04540) yggM 940171..941178 (+) 1008 WP_000745192.1 DUF1202 family protein -
  IR210_RS04545 (IR210_04545) hemW 941247..942383 (-) 1137 WP_194446254.1 radical SAM family heme chaperone HemW -
  IR210_RS04550 (IR210_04550) rdgB 942376..942969 (-) 594 WP_001174738.1 XTP/dITP diphosphatase -
  IR210_RS04555 (IR210_04555) yggU 942977..943267 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  IR210_RS04560 (IR210_04560) yggT 943264..943830 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  IR210_RS04565 (IR210_04565) yggS 943848..944552 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  IR210_RS04570 (IR210_04570) pilT 944570..945550 (+) 981 WP_001443100.1 type IV pilus twitching motility protein PilT Machinery gene
  IR210_RS04575 (IR210_04575) ruvX 945724..946141 (-) 418 Protein_916 Holliday junction resolvase RuvX -
  IR210_RS04580 (IR210_04580) yqgE 946141..946704 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  IR210_RS04585 (IR210_04585) gshB 946813..947763 (-) 951 WP_000593259.1 glutathione synthase -
  IR210_RS04590 (IR210_04590) rsmE 947776..948507 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  IR210_RS04595 (IR210_04595) endA 948587..949294 (-) 708 WP_001327408.1 deoxyribonuclease I -
  IR210_RS04600 (IR210_04600) yggI 949389..949886 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36073.30 Da        Isoelectric Point: 5.9975

>NTDB_id=441124 IR210_RS04570 WP_001443100.1 944570..945550(+) (pilT) [Escherichia coli strain 25DN]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFTSGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VKEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=441124 IR210_RS04570 WP_001443100.1 944570..945550(+) (pilT) [Escherichia coli strain 25DN]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATACGCGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACTTTCACATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCTGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGTTGTCACAAAAACTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGAAAGAAGGGCGCTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362