Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HH212_RS03930 Genome accession   NZ_CP051685
Coordinates   910691..911734 (+) Length   347 a.a.
NCBI ID   WP_169434184.1    Uniprot ID   A0A7Z2VTQ4
Organism   Massilia forsythiae strain GN2-R2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 905691..916734
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HH212_RS03895 (HH212_03895) - 905731..906681 (+) 951 WP_169434177.1 hydrogen peroxide-inducible genes activator -
  HH212_RS03900 (HH212_03900) ubiA 906900..907754 (+) 855 WP_169434178.1 4-hydroxybenzoate octaprenyltransferase -
  HH212_RS03905 (HH212_03905) - 907853..908173 (+) 321 WP_169434179.1 YqjD family protein -
  HH212_RS03910 (HH212_03910) - 908173..908598 (+) 426 WP_169434180.1 phage holin family protein -
  HH212_RS03915 (HH212_03915) - 908591..908908 (+) 318 WP_169434181.1 hypothetical protein -
  HH212_RS03920 (HH212_03920) proC 909051..909869 (-) 819 WP_169434182.1 pyrroline-5-carboxylate reductase -
  HH212_RS03925 (HH212_03925) - 909901..910602 (-) 702 WP_169434183.1 YggS family pyridoxal phosphate-dependent enzyme -
  HH212_RS03930 (HH212_03930) pilT 910691..911734 (+) 1044 WP_169434184.1 type IV pilus twitching motility protein PilT Machinery gene
  HH212_RS03935 (HH212_03935) pilU 911752..912888 (+) 1137 WP_169434185.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HH212_RS03940 (HH212_03940) - 913145..913630 (+) 486 WP_308633268.1 glutathione peroxidase -
  HH212_RS03945 (HH212_03945) - 913913..915583 (+) 1671 WP_169434187.1 glycoside hydrolase family 28 protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38068.79 Da        Isoelectric Point: 6.7805

>NTDB_id=440453 HH212_RS03930 WP_169434184.1 910691..911734(+) (pilT) [Massilia forsythiae strain GN2-R2]
MDISELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPPLEHKDVHGMIYDIMNDGQRKQYEEMLECDFSFAIPGLAR
FRVNAYNQERGAAAVLRTIPSKILSLEELNAPKIFAELALKPRGLVLVTGPTGSGKSTTLAAMVNHLNEHEYGHILTIED
PIEFVHDSKKCLINQREVGPHTLSFNNALRSALREDPDAILVGELRDLETIRLALSAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAEEKEMVRAMLSESLQAVISQTLLKTKDGSGRVAAHEIMIGTPAIRNLIREAKIAQMYSAIQTGSNAGMQTLDQN
LTDLVRRNVISSMAARSAAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=440453 HH212_RS03930 WP_169434184.1 910691..911734(+) (pilT) [Massilia forsythiae strain GN2-R2]
ATGGACATTTCGGAACTCCTCGCTTTCTCCGTGAAGAACAAGGCGTCGGACCTGCACCTCTCGGCCGGCCTGCCGCCGAT
GATCCGCGTGCACGGCGACGTCCGCCGCATCAACCTGCCGCCGCTGGAGCACAAGGACGTGCACGGCATGATCTATGACA
TCATGAACGACGGCCAGCGCAAGCAGTACGAGGAAATGCTGGAGTGCGACTTCTCGTTCGCGATTCCCGGCCTGGCGCGC
TTCCGCGTCAACGCGTACAACCAGGAACGCGGCGCCGCCGCGGTGCTGCGCACGATTCCCTCGAAGATCCTGTCGCTGGA
AGAATTGAACGCACCGAAGATCTTCGCCGAACTGGCCTTGAAGCCGCGCGGCCTGGTGCTGGTGACCGGCCCTACCGGTT
CCGGCAAGTCGACCACGCTGGCGGCGATGGTGAACCACCTGAACGAGCACGAGTACGGCCACATCCTCACCATCGAAGAC
CCGATCGAATTCGTGCACGATTCCAAGAAATGCCTGATCAACCAGCGCGAGGTCGGACCGCACACGCTGTCCTTCAACAA
CGCGCTGCGCTCGGCGCTGCGCGAAGACCCGGACGCGATCCTGGTGGGCGAACTGCGCGACCTGGAGACCATCCGCCTGG
CGCTGTCGGCCGCCGAGACCGGCCACCTGGTGTTCGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
GTCGACGTGTTCCCGGCCGAAGAGAAGGAAATGGTGCGCGCGATGTTGTCGGAATCGCTGCAGGCGGTGATCTCACAGAC
GCTGCTCAAGACCAAGGACGGGTCCGGCCGCGTGGCCGCGCACGAGATCATGATCGGTACCCCGGCGATCCGCAACCTGA
TCCGTGAAGCCAAGATCGCCCAGATGTACTCGGCGATCCAGACCGGTTCCAACGCCGGCATGCAGACCCTCGACCAGAAC
CTGACCGACCTGGTGCGCCGCAACGTCATCTCCAGCATGGCCGCGCGCAGCGCCGCCAAGATCCCCGAAAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z2VTQ4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

77.101

99.424

0.767
  pilT Acinetobacter baumannii strain A118

77.101

99.424

0.767
  pilT Pseudomonas aeruginosa PAK

77.101

99.424

0.767
  pilT Acinetobacter nosocomialis M2

77.101

99.424

0.767
  pilT Pseudomonas stutzeri DSM 10701

75.072

99.424

0.746
  pilT Acinetobacter baylyi ADP1

74.783

99.424

0.744
  pilT Legionella pneumophila strain Lp02

75.516

97.695

0.738
  pilT Legionella pneumophila strain ERS1305867

75.516

97.695

0.738
  pilT Neisseria gonorrhoeae MS11

69.565

99.424

0.692
  pilT Neisseria meningitidis 8013

69.565

99.424

0.692
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.118

0.666
  pilT Vibrio cholerae strain A1552

68.546

97.118

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

99.424

0.51
  pilU Acinetobacter baylyi ADP1

42.197

99.712

0.421
  pilU Vibrio cholerae strain A1552

41.003

97.695

0.401
  pilU Pseudomonas stutzeri DSM 10701

42.284

93.372

0.395