Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HH212_RS03935 Genome accession   NZ_CP051685
Coordinates   911752..912888 (+) Length   378 a.a.
NCBI ID   WP_169434185.1    Uniprot ID   A0A7Z2ZRL6
Organism   Massilia forsythiae strain GN2-R2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 906752..917888
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HH212_RS03900 (HH212_03900) ubiA 906900..907754 (+) 855 WP_169434178.1 4-hydroxybenzoate octaprenyltransferase -
  HH212_RS03905 (HH212_03905) - 907853..908173 (+) 321 WP_169434179.1 YqjD family protein -
  HH212_RS03910 (HH212_03910) - 908173..908598 (+) 426 WP_169434180.1 phage holin family protein -
  HH212_RS03915 (HH212_03915) - 908591..908908 (+) 318 WP_169434181.1 hypothetical protein -
  HH212_RS03920 (HH212_03920) proC 909051..909869 (-) 819 WP_169434182.1 pyrroline-5-carboxylate reductase -
  HH212_RS03925 (HH212_03925) - 909901..910602 (-) 702 WP_169434183.1 YggS family pyridoxal phosphate-dependent enzyme -
  HH212_RS03930 (HH212_03930) pilT 910691..911734 (+) 1044 WP_169434184.1 type IV pilus twitching motility protein PilT Machinery gene
  HH212_RS03935 (HH212_03935) pilU 911752..912888 (+) 1137 WP_169434185.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HH212_RS03940 (HH212_03940) - 913145..913630 (+) 486 WP_308633268.1 glutathione peroxidase -
  HH212_RS03945 (HH212_03945) - 913913..915583 (+) 1671 WP_169434187.1 glycoside hydrolase family 28 protein -
  HH212_RS03950 (HH212_03950) - 915599..916753 (-) 1155 WP_169434188.1 epoxide hydrolase family protein -
  HH212_RS03955 (HH212_03955) - 916857..917579 (+) 723 WP_169434189.1 TetR/AcrR family transcriptional regulator -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41960.42 Da        Isoelectric Point: 6.6669

>NTDB_id=440454 HH212_RS03935 WP_169434185.1 911752..912888(+) (pilU) [Massilia forsythiae strain GN2-R2]
MERDQASKFMFDLLRLMVNKGGSDLFITAGFPPAVKIDGRMTPVSSQPLTGAHTADLARAIMNDKQAAGFELSKEANFAI
SPGDLGRFRVSAFMQMGAVGMVLRVITTTIPKFAELDLPEVLKDVIMTKRGLVIMVGATGSGKSTTMAAMVGYRNEHSYG
HIITIEDPVEFVHPHRNCVITQREVGVDTDSFEAALKNSLRQAPDVIQIGEIRDRETMEHAIAFAETGHLCLATLHANSA
NQALDRIINFFPEERRQQLLMDLSLNMKGLISQRLIPRKETKGRVVAIEIMLNSPLISDLIFKGEVHEIKEIMKKSRELG
MQTFDQALFDLYEADKISYEDALRNADSVNDLRLNIKLNSKHAKNRDFSAGTETLGLV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=440454 HH212_RS03935 WP_169434185.1 911752..912888(+) (pilU) [Massilia forsythiae strain GN2-R2]
ATGGAACGCGACCAGGCCTCCAAATTCATGTTCGACCTGCTGCGCCTGATGGTGAACAAGGGCGGCTCCGACCTGTTCAT
CACGGCCGGCTTTCCGCCGGCCGTCAAGATCGACGGCCGCATGACGCCGGTCTCCAGCCAGCCGCTCACCGGCGCCCACA
CGGCCGACCTGGCGCGCGCCATCATGAACGACAAGCAGGCGGCCGGCTTCGAGCTGAGCAAGGAAGCCAACTTCGCCATC
AGCCCGGGCGACCTGGGGCGCTTCCGCGTCTCGGCCTTCATGCAGATGGGGGCGGTCGGCATGGTGCTGCGCGTGATCAC
GACGACCATTCCGAAGTTCGCCGAGCTGGACCTGCCGGAGGTATTGAAAGACGTGATCATGACCAAGCGCGGCCTGGTGA
TCATGGTCGGCGCCACCGGTTCCGGCAAGTCGACCACGATGGCGGCCATGGTCGGCTACCGCAACGAGCACAGCTACGGC
CACATCATCACCATCGAGGACCCGGTCGAATTCGTCCACCCGCACAGGAATTGCGTGATCACCCAGCGCGAGGTCGGCGT
GGACACCGACAGCTTCGAGGCGGCGCTGAAGAACTCGCTGCGCCAGGCGCCGGACGTAATCCAGATCGGCGAAATCCGCG
ACCGCGAAACCATGGAGCACGCGATCGCCTTCGCCGAGACCGGCCATTTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTCGACCGCATCATCAACTTCTTCCCGGAAGAGCGGCGCCAGCAGCTGCTGATGGACCTGTCGCTGAACAT
GAAGGGCCTGATCTCGCAGCGCCTGATCCCGAGGAAGGAAACCAAGGGCCGCGTGGTCGCCATCGAGATCATGCTGAACT
CTCCCTTGATTTCGGACCTGATCTTCAAGGGCGAAGTCCACGAGATCAAGGAAATCATGAAGAAGTCGCGCGAGCTCGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTACGAGGCCGACAAGATTTCGTACGAAGATGCGCTGCGCAACGCCGA
CTCGGTGAACGACCTGCGCCTGAACATCAAGCTCAACAGCAAGCACGCGAAGAACCGCGATTTCTCGGCCGGCACCGAAA
CCCTCGGCCTGGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z2ZRL6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.61

92.328

0.587
  pilU Acinetobacter baylyi ADP1

58.824

94.444

0.556
  pilU Vibrio cholerae strain A1552

57.303

94.18

0.54
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.075

88.624

0.399
  pilT Pseudomonas aeruginosa PAK

42.687

88.624

0.378
  pilT Vibrio cholerae strain A1552

43.81

83.333

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.81

83.333

0.365
  pilT Acinetobacter nosocomialis M2

41.39

87.566

0.362
  pilT Acinetobacter baumannii D1279779

41.39

87.566

0.362
  pilT Acinetobacter baumannii strain A118

41.39

87.566

0.362