Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HH196_RS09625 Genome accession   NZ_CP051666
Coordinates   1981009..1982043 (+) Length   344 a.a.
NCBI ID   WP_169451905.1    Uniprot ID   A0A858QZW3
Organism   Marinobacterium sp. LSUCC0821     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1976009..1987043
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HH196_RS09590 (HH196_09590) - 1976256..1976693 (-) 438 WP_169451899.1 DUF4426 domain-containing protein -
  HH196_RS09595 (HH196_09595) metW 1976703..1977293 (-) 591 WP_169451900.1 methionine biosynthesis protein MetW -
  HH196_RS09600 (HH196_09600) - 1977283..1978449 (-) 1167 WP_169451901.1 homoserine O-acetyltransferase -
  HH196_RS09605 (HH196_09605) - 1978507..1978803 (-) 297 WP_248276849.1 DUF167 family protein -
  HH196_RS09610 (HH196_09610) - 1978817..1979362 (-) 546 WP_169451902.1 YggT family protein -
  HH196_RS09615 (HH196_09615) proC 1979436..1980260 (-) 825 WP_169451903.1 pyrroline-5-carboxylate reductase -
  HH196_RS09620 (HH196_09620) - 1980273..1980965 (-) 693 WP_169451904.1 YggS family pyridoxal phosphate-dependent enzyme -
  HH196_RS09625 (HH196_09625) pilT 1981009..1982043 (+) 1035 WP_169451905.1 type IV pilus twitching motility protein PilT Machinery gene
  HH196_RS09630 (HH196_09630) pilU 1982056..1983192 (+) 1137 WP_169451906.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HH196_RS09635 (HH196_09635) - 1983278..1984555 (-) 1278 WP_169451907.1 dihydroorotase -
  HH196_RS09640 (HH196_09640) - 1984555..1985559 (-) 1005 WP_169451908.1 aspartate carbamoyltransferase catalytic subunit -
  HH196_RS09645 (HH196_09645) pyrR 1985573..1986082 (-) 510 WP_169451909.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  HH196_RS09650 (HH196_09650) ruvX 1986072..1986539 (-) 468 WP_169451910.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38169.75 Da        Isoelectric Point: 6.4368

>NTDB_id=440365 HH196_RS09625 WP_169451905.1 1981009..1982043(+) (pilT) [Marinobacterium sp. LSUCC0821]
MDITELLAFAVQQGASDLHLTAGMPPLIRVDGEVRRIKLPSMEHKEVHALVYEIMNDKQRKEYEENLEADFSFEVPNLSR
FRVNAFNQHRGAGAVFRTVPSKVLTMDQLGMGTVFKQLSDQPRGLVLVTGPTGSGKSTTLAAMIDYINDTRSDHILTIED
PIEFVHESRKSLVNQREVHRDTLSFENALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAAEKDMVRSMLSESLQGVISQTLLKKEGGGRIAAHEIMIGTSAIRNLIREDKVAQMYSAIQTGASVGMKTLDQSL
LELVQKGHITREVARLRAKDPTAF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=440365 HH196_RS09625 WP_169451905.1 1981009..1982043(+) (pilT) [Marinobacterium sp. LSUCC0821]
ATGGATATTACCGAACTTTTGGCCTTTGCAGTACAGCAGGGCGCCTCGGATCTGCATTTAACAGCTGGTATGCCACCTCT
TATTCGAGTTGATGGTGAAGTGCGCCGCATTAAGCTGCCCTCTATGGAGCACAAAGAGGTACATGCGCTTGTCTATGAGA
TTATGAATGACAAGCAGCGTAAAGAGTACGAAGAGAATCTCGAAGCCGATTTCTCGTTTGAGGTACCTAATCTATCTCGC
TTCCGTGTAAATGCGTTTAACCAACACCGTGGTGCGGGTGCCGTATTCCGTACCGTCCCGAGTAAGGTTTTGACGATGGA
TCAGTTAGGCATGGGGACTGTGTTTAAACAGCTCTCCGATCAGCCGCGCGGTCTAGTACTGGTGACGGGTCCGACGGGTT
CAGGTAAATCAACCACACTAGCTGCGATGATCGATTACATTAATGACACTCGCTCTGACCACATTTTGACTATAGAAGAT
CCGATCGAATTTGTTCACGAAAGCCGCAAGAGCTTGGTCAACCAGCGTGAGGTGCATCGCGATACTCTCAGCTTTGAAAA
TGCGCTGCGCTCGGCTCTTCGCGAAGACCCGGACGTTATTCTCGTTGGCGAGATGCGTGACCTAGAGACGATTCGACTTG
CACTAACTGCTGCAGAAACGGGTCACCTAGTCTTTGGTACTCTGCACACCACCTCAGCAGCTAAAACCATCGACCGTATC
ATCGACGTATTCCCAGCCGCCGAGAAAGATATGGTGCGATCTATGCTCTCTGAGTCTCTGCAGGGGGTTATCTCGCAGAC
ACTCCTCAAGAAAGAGGGTGGCGGTCGAATTGCTGCACACGAAATTATGATTGGTACCTCTGCAATCCGTAACCTAATCC
GTGAAGATAAAGTTGCGCAGATGTATTCCGCTATCCAAACCGGCGCCTCGGTTGGTATGAAAACACTGGATCAGTCCCTA
TTGGAGCTGGTTCAGAAAGGTCATATCACACGTGAAGTGGCCCGACTTCGTGCCAAAGACCCAACAGCATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A858QZW3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

81.105

100

0.811
  pilT Acinetobacter nosocomialis M2

79.942

100

0.799
  pilT Acinetobacter baumannii D1279779

79.942

100

0.799
  pilT Acinetobacter baumannii strain A118

79.942

100

0.799
  pilT Acinetobacter baylyi ADP1

79.36

100

0.794
  pilT Pseudomonas stutzeri DSM 10701

79.36

100

0.794
  pilT Legionella pneumophila strain Lp02

70.93

100

0.709
  pilT Legionella pneumophila strain ERS1305867

70.93

100

0.709
  pilT Neisseria meningitidis 8013

67.449

99.128

0.669
  pilT Neisseria gonorrhoeae MS11

66.862

99.128

0.663
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.419

95.058

0.66
  pilT Vibrio cholerae strain A1552

69.419

95.058

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.509
  pilU Pseudomonas stutzeri DSM 10701

42.773

98.547

0.422
  pilU Vibrio cholerae strain A1552

42.09

97.384

0.41
  pilU Acinetobacter baylyi ADP1

39.244

100

0.392