Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HH196_RS09630 Genome accession   NZ_CP051666
Coordinates   1982056..1983192 (+) Length   378 a.a.
NCBI ID   WP_169451906.1    Uniprot ID   A0A858R0D1
Organism   Marinobacterium sp. LSUCC0821     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1977056..1988192
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HH196_RS09600 (HH196_09600) - 1977283..1978449 (-) 1167 WP_169451901.1 homoserine O-acetyltransferase -
  HH196_RS09605 (HH196_09605) - 1978507..1978803 (-) 297 WP_248276849.1 DUF167 family protein -
  HH196_RS09610 (HH196_09610) - 1978817..1979362 (-) 546 WP_169451902.1 YggT family protein -
  HH196_RS09615 (HH196_09615) proC 1979436..1980260 (-) 825 WP_169451903.1 pyrroline-5-carboxylate reductase -
  HH196_RS09620 (HH196_09620) - 1980273..1980965 (-) 693 WP_169451904.1 YggS family pyridoxal phosphate-dependent enzyme -
  HH196_RS09625 (HH196_09625) pilT 1981009..1982043 (+) 1035 WP_169451905.1 type IV pilus twitching motility protein PilT Machinery gene
  HH196_RS09630 (HH196_09630) pilU 1982056..1983192 (+) 1137 WP_169451906.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HH196_RS09635 (HH196_09635) - 1983278..1984555 (-) 1278 WP_169451907.1 dihydroorotase -
  HH196_RS09640 (HH196_09640) - 1984555..1985559 (-) 1005 WP_169451908.1 aspartate carbamoyltransferase catalytic subunit -
  HH196_RS09645 (HH196_09645) pyrR 1985573..1986082 (-) 510 WP_169451909.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  HH196_RS09650 (HH196_09650) ruvX 1986072..1986539 (-) 468 WP_169451910.1 Holliday junction resolvase RuvX -
  HH196_RS09655 (HH196_09655) - 1986551..1987114 (-) 564 WP_169451911.1 YqgE/AlgH family protein -
  HH196_RS09660 (HH196_09660) - 1987163..1988098 (-) 936 WP_169451912.1 energy transducer TonB -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41891.73 Da        Isoelectric Point: 5.9335

>NTDB_id=440366 HH196_RS09630 WP_169451906.1 1982056..1983192(+) (pilU) [Marinobacterium sp. LSUCC0821]
MDFTQLLKVMTERGGSDLFITAGAPPSIKVDGTIKPLTKESLTAAQSRALVYSTMNDKQLAEFEATNECNFALSAHDIGR
FRVSAYVQRNSPGMVLRSIASHIPSMEELKLPEVIKELAMSKRGLILFVGGTSTGKSTSLASMIDYRNENHAGHIITIED
PIEYIHNHKKSIITQREVGLDTDSFETALKNTLRQAPDVILMGEIRSSDTMSYGLAFAETGHLCLATLHANNANQALDRI
ISFFPADHHDQIWMDLSLNLKAIIAQQLVPTKDGKGRRAAIEVMINTPLIQDLIRKGAVHEIKDVIKKSTNLGMKTFDQS
LYDLYREDVITYETALSHADSANDLRLMIKLSADGNEKPTQADDMPSFTLQDEEDKRG

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=440366 HH196_RS09630 WP_169451906.1 1982056..1983192(+) (pilU) [Marinobacterium sp. LSUCC0821]
GTGGACTTTACTCAACTACTTAAAGTGATGACTGAGCGGGGCGGTTCCGATCTATTCATTACAGCAGGCGCGCCGCCATC
GATTAAAGTGGATGGCACCATTAAGCCGCTAACCAAAGAGTCATTAACTGCTGCTCAATCCCGTGCGCTCGTTTATAGCA
CCATGAACGACAAACAATTGGCAGAGTTTGAAGCGACCAATGAGTGTAACTTCGCACTCAGCGCCCATGACATTGGCCGT
TTCCGTGTCAGCGCTTATGTGCAACGCAACTCTCCCGGAATGGTGCTGCGCTCGATCGCTAGCCATATTCCGTCGATGGA
GGAGCTCAAGCTGCCAGAGGTGATTAAAGAGCTGGCGATGAGTAAACGCGGTTTGATTCTGTTTGTGGGTGGTACCTCAA
CCGGTAAATCGACCTCTTTGGCATCCATGATCGATTATCGCAATGAGAATCATGCGGGTCACATCATTACCATCGAAGAT
CCGATCGAGTACATTCATAACCACAAGAAGAGCATCATCACGCAGCGCGAAGTGGGATTGGATACCGACTCATTTGAAAC
TGCGTTAAAGAACACACTTCGCCAAGCGCCGGATGTCATTCTGATGGGTGAGATTCGCTCGTCAGATACGATGAGTTATG
GCTTAGCTTTTGCCGAAACAGGCCATCTCTGTTTGGCAACGCTTCACGCTAACAACGCCAACCAAGCGTTGGATCGCATC
ATCTCATTCTTCCCAGCAGATCATCACGATCAGATCTGGATGGATCTATCGTTGAACCTGAAAGCGATCATTGCTCAGCA
GTTGGTGCCAACTAAAGATGGGAAAGGTCGACGCGCTGCGATTGAAGTAATGATTAATACCCCGTTGATACAGGATCTGA
TTCGCAAAGGTGCGGTACATGAGATCAAGGATGTGATTAAGAAGTCGACGAACCTGGGTATGAAAACCTTTGATCAGTCG
CTCTATGATCTCTATCGAGAAGATGTCATTACCTACGAAACCGCCCTCAGTCATGCCGACTCAGCGAATGACCTGCGTTT
GATGATTAAACTCAGTGCAGATGGTAACGAAAAACCAACTCAAGCTGATGATATGCCCTCTTTTACCCTCCAGGATGAAG
AGGATAAACGCGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(54-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A858R0D1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.644

93.651

0.643
  pilU Acinetobacter baylyi ADP1

62.5

95.238

0.595
  pilU Vibrio cholerae strain A1552

54.301

98.413

0.534
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.658

89.683

0.392
  pilT Acinetobacter nosocomialis M2

43.658

89.683

0.392
  pilT Acinetobacter baumannii D1279779

43.658

89.683

0.392
  pilT Acinetobacter baumannii strain A118

43.658

89.683

0.392
  pilT Pseudomonas aeruginosa PAK

43.068

89.683

0.386
  pilT Acinetobacter baylyi ADP1

41.935

90.212

0.378
  pilT Pseudomonas stutzeri DSM 10701

42.09

88.624

0.373
  pilT Legionella pneumophila strain Lp02

41.791

88.624

0.37
  pilT Legionella pneumophila strain ERS1305867

41.791

88.624

0.37
  pilT Neisseria meningitidis 8013

40.29

91.27

0.368
  pilT Neisseria gonorrhoeae MS11

40.29

91.27

0.368