Detailed information NTG0439854

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: IQS23_RS22015
• Genome accession: NZ_CP063476
• Coordinates: 4608978..4609958 (+)
• Length: 326 a.a.
• NCBI ID: WP_072023908.1
• Uniprot ID: -
• Organism: Escherichia coli strain EM10-18-28
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4603978..4614958

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IQS23_RS21985 (IQS23_21995)	yggM	4604579..4605586 (+)	1008	WP_032152713.1	DUF1202 family protein	-
IQS23_RS21990 (IQS23_22000)	hemW	4605655..4606791 (-)	1137	WP_032152712.1	radical SAM family heme chaperone HemW	-
IQS23_RS21995 (IQS23_22005)	rdgB	4606784..4607377 (-)	594	WP_001174738.1	XTP/dITP diphosphatase	-
IQS23_RS22000 (IQS23_22010)	yggU	4607385..4607675 (-)	291	WP_001277229.1	DUF167 family protein YggU	-
IQS23_RS22005 (IQS23_22015)	yggT	4607672..4608238 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
IQS23_RS22010 (IQS23_22020)	yggS	4608256..4608960 (-)	705	WP_032152711.1	pyridoxal phosphate homeostasis protein	-
IQS23_RS22015 (IQS23_22025)	pilT	4608978..4609958 (+)	981	WP_072023908.1	type IV pilus twitching motility protein PilT	Machinery gene
IQS23_RS22020 (IQS23_22030)	ruvX	4610073..4610489 (-)	417	WP_000017111.1	Holliday junction resolvase RuvX	-
IQS23_RS22025 (IQS23_22035)	yqgE	4610489..4611052 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
IQS23_RS22030 (IQS23_22040)	gshB	4611161..4612111 (-)	951	WP_000593273.1	glutathione synthase	-
IQS23_RS22035 (IQS23_22045)	rsmE	4612124..4612855 (-)	732	WP_001222509.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
IQS23_RS22040 (IQS23_22050)	endA	4612935..4613642 (-)	708	WP_000286497.1	deoxyribonuclease I	-
IQS23_RS22045 (IQS23_22055)	yggI	4613737..4614234 (-)	498	WP_032152710.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35958.12 Da
• Isoelectric Point: 5.5860

>NTDB_id=439854 IQS23_RS22015 WP_072023908.1 4608978..4609958(+) (pilT) [Escherichia coli strain EM10-18-28]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLVNTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQW
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=439854 IQS23_RS22015 WP_072023908.1 4608978..4609958(+) (pilT) [Escherichia coli strain EM10-18-28]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGCGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTCGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCTGCGTTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTAACAG
CGGCAGAAACCGGACACCTGGTGCTGGCAACTTTACATACGCGTGGTGCGGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCAGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGGTTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACTTTTCAGCAGAGTTATCAGCAGTGG
GTGGGGGAAGGACGTTTGTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.476	100	0.488
pilT	Neisseria gonorrhoeae MS11	48.171	100	0.485
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter baumannii strain A118	46.177	100	0.463
pilT	Acinetobacter baumannii D1279779	46.177	100	0.463
pilT	Acinetobacter nosocomialis M2	46.177	100	0.463
pilT	Pseudomonas stutzeri DSM 10701	46.177	100	0.463
pilT	Pseudomonas aeruginosa PAK	45.872	100	0.46
pilT	Legionella pneumophila strain ERS1305867	45.26	100	0.454
pilT	Legionella pneumophila strain Lp02	45.26	100	0.454
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.138	97.546	0.411
pilU	Vibrio cholerae strain A1552	40.694	97.239	0.396
pilU	Pseudomonas stutzeri DSM 10701	38.037	100	0.38
pilU	Acinetobacter baylyi ADP1	36.646	98.773	0.362
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362