Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HEP74_RS06435 Genome accession   NZ_CP051190
Coordinates   1548417..1549454 (+) Length   345 a.a.
NCBI ID   WP_185815705.1    Uniprot ID   -
Organism   Xanthomonas sp. SS     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1543417..1554454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HEP74_RS06410 (HEP74_01256) soxR 1544058..1544495 (+) 438 WP_185815702.1 redox-sensitive transcriptional activator SoxR -
  HEP74_RS06415 (HEP74_01258) - 1544852..1545310 (-) 459 WP_255427903.1 DUF4426 domain-containing protein -
  HEP74_RS06420 (HEP74_01259) proC 1545324..1546166 (-) 843 WP_185815703.1 pyrroline-5-carboxylate reductase -
  HEP74_RS06425 (HEP74_01260) - 1546316..1547344 (-) 1029 WP_185816528.1 hypothetical protein -
  HEP74_RS06430 (HEP74_01261) - 1547642..1548328 (-) 687 WP_185815704.1 YggS family pyridoxal phosphate-dependent enzyme -
  HEP74_RS06435 (HEP74_01262) pilT 1548417..1549454 (+) 1038 WP_185815705.1 type IV pilus twitching motility protein PilT Machinery gene
  HEP74_RS06440 (HEP74_01263) pilU 1549554..1550684 (+) 1131 WP_185815706.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HEP74_RS06445 (HEP74_01264) - 1550842..1552110 (+) 1269 WP_185815707.1 PQQ-binding-like beta-propeller repeat protein -
  HEP74_RS06450 (HEP74_01265) - 1552107..1553603 (+) 1497 WP_185816529.1 diguanylate cyclase -
  HEP74_RS06455 (HEP74_01266) - 1553714..1554256 (+) 543 WP_255427905.1 MFS transporter permease -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38551.20 Da        Isoelectric Point: 6.6653

>NTDB_id=436754 HEP74_RS06435 WP_185815705.1 1548417..1549454(+) (pilT) [Xanthomonas sp. SS]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQTGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRVFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=436754 HEP74_RS06435 WP_185815705.1 1548417..1549454(+) (pilT) [Xanthomonas sp. SS]
ATGGATATCGCTGAACTATTGGCGTTCTCGGTCAAGAACAAGGCATCCGACCTGCACCTGTCCGCCGGCCTGCCGCCGAT
GATCCGGGTCGATGGCGACGTGCGCCGCATCAACATCCCGGCGCTAGACCACAAACAGGTGCACGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGCCGCGACTACGAAGAGTTCCTCGAAGTCGACTTCTCGTTCGAGATTCCCAGCCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGCACCATTCCCTCGGAAGTGCTGACCCTGGA
AGACCTGGGCTGCCCGCCGATCTTCCGCCAGCTGATCGACCAGCCGCAGGGCCTGATCCTGGTCACCGGGCCGACCGGCT
CGGGCAAGTCGACCACGCTCGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCACGGCTTCAACGA
GGCGCTGCGCTCGGCGCTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCTGCGGAAACCGGGCACCTGGTGTTTGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTGATCTCGCAGGC
ACTGCTGAAGAAGGTCGGCGGCGGTCGCACCGCGGCCTGGGAGATCATGGTCGGCACCCCGGCGATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACCGGCCAGCAGACCGGCATGCAGACCCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACCCGCAACCAGGCGCGCGAGTATGCCAAGGACAAGCGTGTGTTCGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.233

99.71

0.8
  pilT Acinetobacter baumannii D1279779

80.233

99.71

0.8
  pilT Acinetobacter baumannii strain A118

80.233

99.71

0.8
  pilT Pseudomonas stutzeri DSM 10701

79.07

99.71

0.788
  pilT Acinetobacter baylyi ADP1

78.488

99.71

0.783
  pilT Pseudomonas aeruginosa PAK

79.351

98.261

0.78
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Vibrio cholerae strain A1552

40.597

97.101

0.394