Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HEP74_RS06440 Genome accession   NZ_CP051190
Coordinates   1549554..1550684 (+) Length   376 a.a.
NCBI ID   WP_185815706.1    Uniprot ID   A0A7G7SUL6
Organism   Xanthomonas sp. SS     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1544554..1555684
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HEP74_RS06415 (HEP74_01258) - 1544852..1545310 (-) 459 WP_255427903.1 DUF4426 domain-containing protein -
  HEP74_RS06420 (HEP74_01259) proC 1545324..1546166 (-) 843 WP_185815703.1 pyrroline-5-carboxylate reductase -
  HEP74_RS06425 (HEP74_01260) - 1546316..1547344 (-) 1029 WP_185816528.1 hypothetical protein -
  HEP74_RS06430 (HEP74_01261) - 1547642..1548328 (-) 687 WP_185815704.1 YggS family pyridoxal phosphate-dependent enzyme -
  HEP74_RS06435 (HEP74_01262) pilT 1548417..1549454 (+) 1038 WP_185815705.1 type IV pilus twitching motility protein PilT Machinery gene
  HEP74_RS06440 (HEP74_01263) pilU 1549554..1550684 (+) 1131 WP_185815706.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HEP74_RS06445 (HEP74_01264) - 1550842..1552110 (+) 1269 WP_185815707.1 PQQ-binding-like beta-propeller repeat protein -
  HEP74_RS06450 (HEP74_01265) - 1552107..1553603 (+) 1497 WP_185816529.1 diguanylate cyclase -
  HEP74_RS06455 (HEP74_01266) - 1553714..1554256 (+) 543 WP_255427905.1 MFS transporter permease -
  HEP74_RS06460 (HEP74_01267) - 1554426..1555100 (+) 675 WP_185816530.1 YitT family protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41932.26 Da        Isoelectric Point: 6.8811

>NTDB_id=436755 HEP74_RS06440 WP_185815706.1 1549554..1550684(+) (pilU) [Xanthomonas sp. SS]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKITPITQTPLTSQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRSQLLMDLSLNMRGVVAQQLIPTPDGKGRRVAMEIMLGTPLVQDYIREGEIHKLKDVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRHADSQNEVRLRIKLAQGGDAKTLAQGMDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=436755 HEP74_RS06440 WP_185815706.1 1549554..1550684(+) (pilU) [Xanthomonas sp. SS]
ATGAGCACCATCGACTTCACCTCGTTCCTGAAACTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACTTCGGGCAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCACCCCGATCACGCAGACGCCGCTGACCTCGCAGCAGAGCCGCGACCTGG
TGCTGAACGTGATGACTCCGGCGCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCGTGGCCGGG
GTCGGGCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGACGCGCATTCC
CACCGTCGACGAGCTGAACCTGCCGCCGGTGATCAAGACCCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGTTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGCCACATCATCACC
ATCGAAGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTGAAGAACACTCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCACGCGATCTCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAGGACCGCCGCAGCCAGCTGCTGATGGATCTGTCGCTGAACATGCGCGGCGTGGT
CGCCCAGCAGCTGATTCCCACCCCCGACGGCAAGGGCCGGCGCGTGGCGATGGAGATCATGCTCGGCACGCCGCTGGTGC
AGGACTACATCCGCGAGGGCGAGATCCACAAGCTCAAGGACGTGATGAAGGAATCCACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAAGACGCGCTGCGCCACGCCGACTCGCAGAACGA
AGTGCGCCTGCGCATCAAGCTCGCCCAGGGCGGCGACGCCAAGACCCTGGCGCAGGGCATGGACGGGGTGGAGATCGCCG
AGGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G7SUL6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.583

98.138

0.644
  pilU Acinetobacter baylyi ADP1

63.305

94.947

0.601
  pilU Vibrio cholerae strain A1552

53.371

94.681

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.286

93.085

0.394
  pilT Pseudomonas aeruginosa PAK

40.708

90.16

0.367
  pilT Acinetobacter nosocomialis M2

40.116

91.489

0.367
  pilT Acinetobacter baumannii D1279779

40.116

91.489

0.367
  pilT Acinetobacter baumannii strain A118

40.116

91.489

0.367