Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HF888_RS01235 Genome accession   NZ_CP051183
Coordinates   276905..277939 (+) Length   344 a.a.
NCBI ID   WP_007018002.1    Uniprot ID   Q1N4D4
Organism   Bermanella marisrubri strain RED65     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 271905..282939
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF888_RS01215 (HF888_01215) - 271958..274261 (+) 2304 WP_007018006.1 sensor histidine kinase -
  HF888_RS01220 (HF888_01220) - 274327..275166 (+) 840 WP_007018005.1 DUF6279 family lipoprotein -
  HF888_RS01225 (HF888_01225) proC 275185..276003 (-) 819 WP_007018004.1 pyrroline-5-carboxylate reductase -
  HF888_RS01230 (HF888_01230) - 276013..276729 (-) 717 WP_007018003.1 YggS family pyridoxal phosphate-dependent enzyme -
  HF888_RS01235 (HF888_01235) pilT 276905..277939 (+) 1035 WP_007018002.1 type IV pilus twitching motility protein PilT Machinery gene
  HF888_RS01240 (HF888_01240) pilU 277988..279100 (+) 1113 WP_007018001.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HF888_RS01245 (HF888_01245) - 279209..279580 (+) 372 WP_007018000.1 hypothetical protein -
  HF888_RS01250 (HF888_01250) - 279674..280471 (+) 798 WP_007017999.1 SDR family oxidoreductase -
  HF888_RS01255 (HF888_01255) - 280489..281499 (-) 1011 WP_007017998.1 2Fe-2S iron-sulfur cluster-binding protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37927.52 Da        Isoelectric Point: 6.9774

>NTDB_id=436687 HF888_RS01235 WP_007018002.1 276905..277939(+) (pilT) [Bermanella marisrubri strain RED65]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGEVRRINLPALSHKEVHALVYDIMNDKQRKDFEEFLETDYSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTCDDLGMGQIFKDLATKPRGLVLVTGPTGSGKSTTLAAMLDYLNDNYYKHILTVED
PIEFVHQSKKSLINQREVHRDTLGFNEALRSALREDPDVILVGEMRDLETIRLALTAAETGHCVFGTLHTTSAAKTIDRI
VDVFPAEEKSMVRSMLSESLQGVISQTLLKKKGGGRVAAHEIMVGTPAIRNLIREDKVAQMYSAIQTGGSLGMTTMDQSL
KTLVQKGLVTPESAREKAKNPEQF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=436687 HF888_RS01235 WP_007018002.1 276905..277939(+) (pilT) [Bermanella marisrubri strain RED65]
ATGGATATCACTGAACTACTCGCCTTCTCTGCCAAGCAAGGGGCATCGGATTTACATTTATCGGCCGGTTTGCCACCGAT
GATCCGTGTAGATGGCGAAGTACGTCGGATAAACCTTCCCGCTTTAAGTCATAAAGAAGTGCATGCGCTGGTTTATGACA
TCATGAACGATAAACAGCGTAAGGATTTTGAAGAATTCCTCGAAACCGATTACTCGTTTGAGGTTCCCGGTGTGGCTCGT
TTTCGTGTCAACGCATTTAATCAAAACCGCGGTGCCGGCGCAGTGTTCCGTACTATCCCGTCGAAGGTACTGACATGTGA
TGACTTGGGTATGGGCCAAATCTTTAAAGATTTGGCCACGAAACCCCGTGGCTTGGTGTTGGTAACTGGGCCAACTGGTT
CAGGTAAATCGACCACACTCGCGGCCATGTTGGATTATCTCAATGACAACTACTATAAGCACATCTTAACCGTAGAAGAC
CCTATTGAATTCGTTCACCAAAGCAAGAAGTCGTTGATTAACCAGCGGGAAGTACACCGAGATACCCTAGGTTTTAACGA
AGCATTGCGCTCGGCACTACGTGAAGACCCGGATGTGATCTTGGTTGGTGAGATGCGTGACTTAGAAACAATCCGCTTGG
CGTTGACCGCGGCAGAAACGGGTCACTGTGTGTTTGGTACCTTGCATACTACGAGCGCAGCTAAAACCATTGACCGTATT
GTGGACGTATTCCCTGCTGAAGAAAAATCCATGGTCCGTTCTATGTTGTCAGAATCTCTACAAGGGGTTATTTCTCAAAC
CCTTTTGAAGAAAAAAGGCGGTGGTCGTGTTGCAGCACACGAAATCATGGTCGGTACGCCAGCCATTCGTAATTTGATTC
GGGAAGATAAAGTAGCGCAAATGTACAGCGCCATTCAAACAGGTGGTTCATTAGGCATGACTACAATGGATCAGAGTTTA
AAAACCTTGGTTCAGAAGGGTTTGGTGACACCAGAATCCGCGCGAGAAAAAGCCAAAAACCCAGAGCAATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q1N4D4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

86.919

100

0.869
  pilT Pseudomonas stutzeri DSM 10701

86.337

100

0.863
  pilT Acinetobacter nosocomialis M2

84.884

100

0.849
  pilT Acinetobacter baumannii D1279779

84.884

100

0.849
  pilT Acinetobacter baumannii strain A118

84.884

100

0.849
  pilT Acinetobacter baylyi ADP1

81.395

100

0.814
  pilT Legionella pneumophila strain Lp02

72.384

100

0.724
  pilT Legionella pneumophila strain ERS1305867

72.384

100

0.724
  pilT Neisseria meningitidis 8013

68.805

99.709

0.686
  pilT Neisseria gonorrhoeae MS11

68.513

99.709

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.249

97.965

0.669
  pilT Vibrio cholerae strain A1552

68.249

97.965

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Vibrio cholerae strain A1552

40.708

98.547

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

39.067

99.709

0.39