Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HF888_RS01240 Genome accession   NZ_CP051183
Coordinates   277988..279100 (+) Length   370 a.a.
NCBI ID   WP_007018001.1    Uniprot ID   Q1N4D5
Organism   Bermanella marisrubri strain RED65     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 272988..284100
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF888_RS01220 (HF888_01220) - 274327..275166 (+) 840 WP_007018005.1 DUF6279 family lipoprotein -
  HF888_RS01225 (HF888_01225) proC 275185..276003 (-) 819 WP_007018004.1 pyrroline-5-carboxylate reductase -
  HF888_RS01230 (HF888_01230) - 276013..276729 (-) 717 WP_007018003.1 YggS family pyridoxal phosphate-dependent enzyme -
  HF888_RS01235 (HF888_01235) pilT 276905..277939 (+) 1035 WP_007018002.1 type IV pilus twitching motility protein PilT Machinery gene
  HF888_RS01240 (HF888_01240) pilU 277988..279100 (+) 1113 WP_007018001.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HF888_RS01245 (HF888_01245) - 279209..279580 (+) 372 WP_007018000.1 hypothetical protein -
  HF888_RS01250 (HF888_01250) - 279674..280471 (+) 798 WP_007017999.1 SDR family oxidoreductase -
  HF888_RS01255 (HF888_01255) - 280489..281499 (-) 1011 WP_007017998.1 2Fe-2S iron-sulfur cluster-binding protein -
  HF888_RS01260 (HF888_01260) ubiD 281507..282991 (-) 1485 WP_007017997.1 4-hydroxy-3-polyprenylbenzoate decarboxylase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40961.11 Da        Isoelectric Point: 6.2352

>NTDB_id=436688 HF888_RS01240 WP_007018001.1 277988..279100(+) (pilU) [Bermanella marisrubri strain RED65]
MEIEKLLRLMVEKSASDLFITAGMPPSMKVHGRIHSVTKAPLSPEQTKEIIYGMMSAKQIEEFEELKELNFAVSASGIGR
FRASAFFQRNLAGVVLRRIETRIPTIEQLGLPDVIKDIAMSKRGLVLFVGATGAGKSTSLASMIGYRNQNSRGHIISIED
PIEYMHQHQGCIVTQREVGLDTESFEIALKNTLRQAPDVIMIGEVRSAQTMNHAVTFAETGHLCLATLHANNANQALDRV
IHFFPPEQHSQVWMDLSLNLKAIVAQQLIPTPDGKGRRAVVEVLLNTPLVADMIRKGEVHELKELMTKSTEQGMQTFDQG
LYNLYQAGEITYEEALAHADSANDLRLMIKLASEDADQLSSATKGLTLQE

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=436688 HF888_RS01240 WP_007018001.1 277988..279100(+) (pilU) [Bermanella marisrubri strain RED65]
ATGGAAATCGAAAAACTACTTCGCCTTATGGTGGAAAAAAGCGCATCCGATTTGTTCATTACTGCAGGTATGCCACCTAG
CATGAAAGTTCATGGACGTATTCATTCGGTGACCAAAGCGCCTTTGAGCCCCGAGCAAACCAAAGAAATTATCTATGGCA
TGATGAGCGCAAAACAAATCGAAGAGTTTGAAGAGTTAAAAGAACTGAACTTTGCGGTGAGTGCTAGCGGTATAGGTCGT
TTCCGTGCCAGTGCATTTTTTCAGCGTAACCTTGCTGGCGTTGTGCTACGTCGAATCGAAACGCGTATTCCTACTATTGA
GCAGCTTGGCCTTCCTGATGTCATCAAAGATATCGCCATGAGTAAACGCGGTTTGGTTTTATTTGTGGGTGCAACGGGTG
CGGGTAAATCGACTTCGTTGGCCTCCATGATTGGTTATCGTAACCAAAATAGTCGCGGCCACATTATCAGTATCGAAGAC
CCTATCGAATACATGCACCAGCACCAAGGGTGTATCGTAACGCAACGAGAGGTAGGTCTAGATACGGAAAGCTTTGAGAT
AGCCCTGAAAAATACCCTGCGTCAGGCACCGGATGTGATCATGATTGGTGAGGTGCGTAGTGCGCAAACCATGAATCATG
CGGTTACCTTCGCAGAAACAGGTCACTTATGTTTAGCCACATTGCATGCGAATAATGCTAACCAAGCATTGGATCGCGTT
ATTCACTTTTTCCCGCCAGAACAACATTCACAGGTGTGGATGGATTTGAGTCTTAACTTAAAAGCGATCGTCGCACAGCA
ACTTATTCCAACCCCAGATGGCAAGGGTCGTCGCGCCGTGGTTGAAGTACTATTGAATACACCATTGGTTGCCGACATGA
TTCGCAAAGGTGAGGTTCATGAACTTAAAGAATTGATGACGAAATCCACAGAGCAAGGCATGCAAACCTTTGATCAAGGC
CTCTATAACTTGTATCAAGCTGGTGAGATTACGTATGAAGAAGCGCTTGCCCATGCGGATAGTGCAAACGACTTACGTTT
GATGATTAAACTTGCGTCTGAAGATGCCGATCAGTTATCCAGCGCCACTAAAGGCTTAACCCTACAAGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q1N4D5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

76.216

100

0.762
  pilU Acinetobacter baylyi ADP1

60.563

95.946

0.581
  pilU Vibrio cholerae strain A1552

58.31

95.946

0.559
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.526

98.108

0.427
  pilT Legionella pneumophila strain Lp02

41.493

90.541

0.376
  pilT Legionella pneumophila strain ERS1305867

41.493

90.541

0.376
  pilT Pseudomonas aeruginosa PAK

40.896

90.541

0.37
  pilT Acinetobacter nosocomialis M2

40

90.541

0.362
  pilT Acinetobacter baumannii D1279779

40

90.541

0.362
  pilT Acinetobacter baumannii strain A118

40

90.541

0.362
  pilT Pseudomonas stutzeri DSM 10701

40

90.541

0.362