Detailed information NTG0436166

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: IL980_RS15975
• Genome accession: NZ_CP063046
• Coordinates: 3288366..3289346 (+)
• Length: 326 a.a.
• NCBI ID: WP_001467825.1
• Uniprot ID: -
• Organism: Escherichia coli strain KC-Dl-1
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3283366..3294346

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IL980_RS15945 (IL980_15945)	yggM	3283971..3284978 (+)	1008	WP_021580233.1	DUF1202 family protein	-
IL980_RS15950 (IL980_15950)	hemW	3285043..3286179 (-)	1137	WP_000239951.1	radical SAM family heme chaperone HemW	-
IL980_RS15955 (IL980_15955)	rdgB	3286172..3286765 (-)	594	WP_001174738.1	XTP/dITP diphosphatase	-
IL980_RS15960 (IL980_15960)	yggU	3286773..3287063 (-)	291	WP_001277222.1	DUF167 family protein YggU	-
IL980_RS15965 (IL980_15965)	yggT	3287060..3287626 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
IL980_RS15970 (IL980_15970)	yggS	3287644..3288348 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
IL980_RS15975 (IL980_15975)	pilT	3288366..3289346 (+)	981	WP_001467825.1	type IV pilus twitching motility protein PilT	Machinery gene
IL980_RS15980 (IL980_15980)	ruvX	3289520..3289936 (-)	417	WP_000017106.1	Holliday junction resolvase RuvX	-
IL980_RS15985 (IL980_15985)	yqgE	3289936..3290499 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
IL980_RS15990 (IL980_15990)	gshB	3290608..3291558 (-)	951	WP_000593256.1	glutathione synthase	-
IL980_RS15995 (IL980_15995)	rsmE	3291571..3292302 (-)	732	WP_001222509.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
IL980_RS16000 (IL980_16000)	endA	3292382..3293089 (-)	708	WP_001305312.1	deoxyribonuclease I	-
IL980_RS16005 (IL980_16005)	yggI	3293184..3293681 (-)	498	WP_000858396.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 36073.30 Da
• Isoelectric Point: 5.9975

>NTDB_id=436166 IL980_RS15975 WP_001467825.1 3288366..3289346(+) (pilT) [Escherichia coli strain KC-Dl-1]
MNMEEIVTLSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VKEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=436166 IL980_RS15975 WP_001467825.1 3288366..3289346(+) (pilT) [Escherichia coli strain KC-Dl-1]
ATGAATATGGAAGAAATTGTGACCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGAATGGAAGCTGCGCCGTTTGACGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACTTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCTGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGTTGTCACAAAAACTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGAAAGAAGGGCGCTTGTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.476	100	0.488
pilT	Neisseria gonorrhoeae MS11	48.171	100	0.485
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter baumannii D1279779	46.177	100	0.463
pilT	Acinetobacter nosocomialis M2	46.177	100	0.463
pilT	Acinetobacter baumannii strain A118	46.177	100	0.463
pilT	Pseudomonas stutzeri DSM 10701	46.177	100	0.463
pilT	Pseudomonas aeruginosa PAK	45.872	100	0.46
pilT	Legionella pneumophila strain ERS1305867	44.954	100	0.451
pilT	Legionella pneumophila strain Lp02	44.954	100	0.451
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.138	97.546	0.411
pilU	Vibrio cholerae strain A1552	39.514	100	0.399
pilU	Pseudomonas stutzeri DSM 10701	37.576	100	0.38
pilU	Acinetobacter baylyi ADP1	36.957	98.773	0.365
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362