Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GU3_RS15895 Genome accession   NC_016745
Coordinates   3384735..3385769 (-) Length   344 a.a.
NCBI ID   WP_014293554.1    Uniprot ID   H2FVB0
Organism   Oceanimonas sp. GK1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3379735..3390769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GU3_RS15875 (GU3_15455) - 3380614..3381321 (-) 708 WP_014293550.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  GU3_RS15880 (GU3_15460) srmB 3381392..3382690 (+) 1299 WP_014293551.1 ATP-dependent RNA helicase SrmB -
  GU3_RS15885 (GU3_15465) yaaA 3382818..3383597 (-) 780 WP_014293552.1 peroxide stress protein YaaA -
  GU3_RS15890 (GU3_15470) pilU 3383605..3384714 (-) 1110 WP_014293553.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GU3_RS15895 (GU3_15475) pilT 3384735..3385769 (-) 1035 WP_014293554.1 type IV pilus twitching motility protein PilT Machinery gene
  GU3_RS15900 (GU3_15480) - 3385810..3386502 (+) 693 WP_014293555.1 YggS family pyridoxal phosphate-dependent enzyme -
  GU3_RS15905 (GU3_15485) proC 3386513..3387337 (+) 825 WP_014293556.1 pyrroline-5-carboxylate reductase -
  GU3_RS15910 (GU3_15490) - 3387347..3387898 (+) 552 WP_014293557.1 YggT family protein -
  GU3_RS15915 (GU3_15495) - 3387886..3388197 (+) 312 WP_014293558.1 DUF167 family protein -
  GU3_RS15920 (GU3_15500) - 3388235..3388648 (+) 414 WP_014293559.1 DUF4426 domain-containing protein -
  GU3_RS15925 (GU3_15505) rdgB 3388742..3389374 (+) 633 WP_050899381.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  GU3_RS15930 (GU3_15510) hemW 3389374..3390519 (+) 1146 WP_014293561.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38212.80 Da        Isoelectric Point: 6.9131

>NTDB_id=43286 GU3_RS15895 WP_014293554.1 3384735..3385769(-) (pilT) [Oceanimonas sp. GK1]
MDITELLAFSVKHNASDLHLSAGVQPLLRVDGEVRKINLPVLDHREVHRLVYDIMNDRQRKELEENFEVDFSFELPGLAR
FRVNAYHQARGAAAVFRVIPSEVWTLAQLGGPEIFERIAEYSRGLVLVTGPTGSGKSTTLAAMVNHINDHFNKHILTIED
PIEFVHDNKKCLVNQREVHRDTHSFSNALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQNLLKKVSGGRVAAHEIMLGTPAIRNLIREDKVAQMYSVIQTGMAHGMQTMDQSL
KRLVASGLVSPADARTKAVDPQSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=43286 GU3_RS15895 WP_014293554.1 3384735..3385769(-) (pilT) [Oceanimonas sp. GK1]
ATGGATATTACGGAATTACTGGCGTTCAGTGTAAAGCATAATGCCTCGGATCTGCACCTCTCCGCCGGGGTGCAGCCGCT
GCTGCGGGTGGACGGCGAGGTACGCAAGATCAACCTGCCGGTGCTGGACCACCGGGAAGTGCACCGGCTGGTGTACGACA
TCATGAACGATCGCCAGCGCAAGGAGCTGGAAGAAAACTTCGAGGTCGACTTCTCCTTTGAGCTGCCGGGACTGGCGCGC
TTTCGGGTCAATGCCTATCACCAGGCCCGGGGCGCGGCGGCGGTATTCCGGGTGATCCCGAGCGAAGTGTGGACCCTGGC
GCAGCTCGGCGGGCCGGAGATCTTTGAGCGCATTGCGGAATATTCCCGCGGCCTGGTGCTGGTCACCGGCCCCACCGGCT
CGGGCAAGTCCACCACCCTGGCGGCCATGGTCAACCACATCAACGACCACTTCAACAAGCATATTCTGACCATCGAAGAT
CCCATCGAATTCGTGCACGACAACAAGAAGTGCCTGGTCAACCAGCGGGAAGTGCACCGGGATACCCATTCCTTCAGCAA
TGCCCTGCGCTCGGCGCTGCGGGAAGATCCCGACATCATACTGGTGGGGGAACTGCGGGATCTGGAAACCATTCGCCTGG
CGCTGACCGCCGCCGAAACCGGTCATCTGGTGTTCGGCACCCTGCATACCTCGTCGGCGGCCAAGACCATCGACCGTATC
ATCGACGTTTTTCCCGGCGCCGAAAAGGACATGGTGCGTTCCATGCTGTCGGAATCGCTGCGGGCGGTGATCTCCCAGAA
CCTGCTGAAGAAGGTCAGCGGCGGCCGGGTGGCGGCCCACGAGATCATGCTGGGCACCCCGGCCATTCGCAACCTGATCC
GGGAAGACAAGGTGGCGCAAATGTATTCGGTGATCCAGACCGGCATGGCCCATGGCATGCAGACCATGGATCAGTCCCTG
AAGCGGCTGGTGGCGAGCGGACTGGTGTCTCCCGCCGATGCCCGTACCAAGGCGGTGGATCCCCAGAGCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB H2FVB0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

77.448

97.965

0.759
  pilT Vibrio cholerae strain A1552

77.448

97.965

0.759
  pilT Acinetobacter baumannii D1279779

75.581

100

0.756
  pilT Acinetobacter baumannii strain A118

75.581

100

0.756
  pilT Acinetobacter nosocomialis M2

75.291

100

0.753
  pilT Acinetobacter baylyi ADP1

74.709

100

0.747
  pilT Pseudomonas aeruginosa PAK

74.128

100

0.741
  pilT Pseudomonas stutzeri DSM 10701

74.128

100

0.741
  pilT Legionella pneumophila strain Lp02

71.765

98.837

0.709
  pilT Legionella pneumophila strain ERS1305867

71.765

98.837

0.709
  pilT Neisseria meningitidis 8013

66.957

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.312

99.709

0.512
  pilU Vibrio cholerae strain A1552

41.194

97.384

0.401
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.299

97.384

0.392

Multiple sequence alignment