Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GU3_RS15890 Genome accession   NC_016745
Coordinates   3383605..3384714 (-) Length   369 a.a.
NCBI ID   WP_014293553.1    Uniprot ID   H2FVA9
Organism   Oceanimonas sp. GK1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3378605..3389714
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GU3_RS15870 (GU3_15450) brnQ 3379145..3380419 (-) 1275 WP_162470462.1 branched-chain amino acid transport system II carrier protein -
  GU3_RS15875 (GU3_15455) - 3380614..3381321 (-) 708 WP_014293550.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  GU3_RS15880 (GU3_15460) srmB 3381392..3382690 (+) 1299 WP_014293551.1 ATP-dependent RNA helicase SrmB -
  GU3_RS15885 (GU3_15465) yaaA 3382818..3383597 (-) 780 WP_014293552.1 peroxide stress protein YaaA -
  GU3_RS15890 (GU3_15470) pilU 3383605..3384714 (-) 1110 WP_014293553.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GU3_RS15895 (GU3_15475) pilT 3384735..3385769 (-) 1035 WP_014293554.1 type IV pilus twitching motility protein PilT Machinery gene
  GU3_RS15900 (GU3_15480) - 3385810..3386502 (+) 693 WP_014293555.1 YggS family pyridoxal phosphate-dependent enzyme -
  GU3_RS15905 (GU3_15485) proC 3386513..3387337 (+) 825 WP_014293556.1 pyrroline-5-carboxylate reductase -
  GU3_RS15910 (GU3_15490) - 3387347..3387898 (+) 552 WP_014293557.1 YggT family protein -
  GU3_RS15915 (GU3_15495) - 3387886..3388197 (+) 312 WP_014293558.1 DUF167 family protein -
  GU3_RS15920 (GU3_15500) - 3388235..3388648 (+) 414 WP_014293559.1 DUF4426 domain-containing protein -
  GU3_RS15925 (GU3_15505) rdgB 3388742..3389374 (+) 633 WP_050899381.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41281.18 Da        Isoelectric Point: 6.2990

>NTDB_id=43285 GU3_RS15890 WP_014293553.1 3383605..3384714(-) (pilU) [Oceanimonas sp. GK1]
MELALLLGKMCEHKASDMYISVGVAPTIKTQGKLLPIGDHPLNERETLALVESCMTAECRERYHREKEANFAIHDGEHGR
FRVSAFWQQDKAGMVLRRIESRIPTFEELYLPEVLREVAMAKRGLVLFVGGTGSGKSTTQAAMIGHRNRHAGGHILTIED
PVEFVHQHDQSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTEETMAYALSFAETGHLCMATLHANNANQAIDRI
MHLVPENKHRQLLFDLSFNLKAIVAQQLIPTRDGNQRRGAFEILLNTPIVADIIRQGDMHRLKEIMGKSREAGMQTFDQA
LFDLYARQHIGYTEALAYADSPNDLRLMIKLQDGGGIDSGLFDNVTIES

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=43285 GU3_RS15890 WP_014293553.1 3383605..3384714(-) (pilU) [Oceanimonas sp. GK1]
ATGGAGCTGGCCTTATTATTGGGCAAAATGTGCGAGCACAAGGCCTCGGACATGTATATCTCGGTGGGCGTGGCGCCCAC
TATCAAGACCCAGGGCAAACTGCTGCCCATCGGGGACCACCCGCTGAACGAGCGCGAGACGCTGGCGCTGGTGGAGTCCT
GCATGACCGCCGAATGTCGGGAACGTTATCACCGGGAAAAGGAAGCCAACTTCGCCATTCACGACGGCGAGCACGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAAGACAAGGCGGGCATGGTGCTCAGGCGCATCGAGAGCCGCATTCCCACCTTTGA
AGAGCTGTACCTGCCCGAGGTGCTGCGTGAGGTGGCCATGGCCAAGCGCGGCCTGGTGTTGTTTGTGGGGGGCACCGGCA
GCGGCAAATCCACCACCCAGGCGGCCATGATCGGCCACCGCAACCGCCATGCCGGCGGCCATATTCTCACCATTGAGGAT
CCGGTGGAATTCGTGCACCAGCACGATCAGTCGATCATTACCCAGCGGGAGGTGGGCATCGACACCGAATCCTTCGATGC
GGCCCTGAAAAGCTCGCTGCGCCAGGCCCCGGATGTGATCCTGATCGGGGAGATCCGCACCGAGGAGACCATGGCCTACG
CCCTGTCGTTCGCCGAGACCGGCCACTTGTGCATGGCCACCCTGCATGCCAACAACGCCAACCAGGCCATTGACCGCATC
ATGCACCTGGTGCCCGAGAACAAGCATCGCCAGCTGCTGTTTGATCTGTCGTTCAACCTCAAGGCCATCGTGGCCCAGCA
ACTGATCCCCACCCGGGACGGCAACCAGCGCCGGGGCGCCTTTGAGATCCTGCTCAACACTCCCATCGTGGCCGACATCA
TTCGCCAGGGGGACATGCACCGGCTCAAGGAGATCATGGGCAAGTCCCGCGAGGCGGGCATGCAAACCTTTGATCAGGCG
CTGTTTGACCTCTATGCGCGCCAGCATATAGGCTACACCGAAGCCCTGGCCTACGCCGACTCTCCCAACGATCTGCGCTT
GATGATCAAGCTGCAGGACGGCGGCGGAATCGACTCCGGCCTGTTTGACAACGTAACCATCGAATCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(105-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB H2FVA9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

57.609

99.729

0.575
  pilU Pseudomonas stutzeri DSM 10701

58.974

95.122

0.561
  pilU Acinetobacter baylyi ADP1

57.062

95.935

0.547
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.881

90.786

0.398
  pilT Neisseria gonorrhoeae MS11

40.597

90.786

0.369
  pilT Vibrio cholerae strain A1552

41.59

88.618

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.59

88.618

0.369
  pilT Acinetobacter baylyi ADP1

39.474

92.683

0.366
  pilT Legionella pneumophila strain ERS1305867

40.785

89.702

0.366
  pilT Legionella pneumophila strain Lp02

40.785

89.702

0.366
  pilT Pseudomonas aeruginosa PAK

38.841

93.496

0.363

Multiple sequence alignment