Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   G4G31_RS08535 Genome accession   NZ_CP050451
Coordinates   1847612..1848655 (-) Length   347 a.a.
NCBI ID   WP_182991055.1    Uniprot ID   A0A7G5ZB84
Organism   Massilia sp. Se16.2.3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1842612..1853655
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G4G31_RS08515 (G4G31_08520) - 1843997..1844464 (-) 468 WP_182991051.1 chemotaxis protein CheW -
  G4G31_RS08520 (G4G31_08525) - 1844726..1845712 (-) 987 WP_182991052.1 MerR family transcriptional regulator -
  G4G31_RS08525 (G4G31_08530) - 1845806..1846297 (-) 492 WP_182991053.1 glutathione peroxidase -
  G4G31_RS08530 (G4G31_08535) pilU 1846388..1847524 (-) 1137 WP_182991054.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  G4G31_RS08535 (G4G31_08540) pilT 1847612..1848655 (-) 1044 WP_182991055.1 type IV pilus twitching motility protein PilT Machinery gene
  G4G31_RS08540 (G4G31_08545) - 1848747..1849447 (+) 701 Protein_1791 YggS family pyridoxal phosphate-dependent enzyme -
  G4G31_RS08545 (G4G31_08550) proC 1849462..1850277 (+) 816 WP_182991056.1 pyrroline-5-carboxylate reductase -
  G4G31_RS08550 (G4G31_08555) - 1850598..1850894 (-) 297 WP_182991057.1 YqjK family protein -
  G4G31_RS08555 (G4G31_08560) - 1850887..1851264 (-) 378 WP_182991058.1 phage holin family protein -
  G4G31_RS08560 (G4G31_08565) - 1851278..1851595 (-) 318 WP_182991059.1 YqjD family protein -
  G4G31_RS08565 (G4G31_08570) ubiA 1851666..1852520 (-) 855 WP_182991060.1 4-hydroxybenzoate octaprenyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38000.63 Da        Isoelectric Point: 6.7294

>NTDB_id=432832 G4G31_RS08535 WP_182991055.1 1847612..1848655(-) (pilT) [Massilia sp. Se16.2.3]
MDISELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPPLEHKDVHAMVYDIMNDGQRKQYEEVMEVDFSFSIPGLAR
FRVNAFNQERGAAAVLRTIPSKILTLEELNAPKIFGDLALKPRGLVLVTGPTGSGKSTTLAAMVNHLNENEYGHILTIED
PIEFVHESKKCLINQREVGPHTLSFSNSLRSALREDPDAILVGELRDLETIRLALSAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAEEKEMVRAMLSESLQAVISQTLLKTKDGSGRVAAHEIMIGTPAIRNLIREAKIAQMYSAIQTGSNTGMQTLDQN
LTDLVRRNIISSSAARAAAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=432832 G4G31_RS08535 WP_182991055.1 1847612..1848655(-) (pilT) [Massilia sp. Se16.2.3]
ATGGACATCTCAGAATTACTCGCCTTCTCGGTGAAGAACAAGGCTTCCGACTTGCACCTCTCGGCCGGCCTGCCGCCGAT
GATCCGCGTGCATGGCGACGTACGCCGCATCAACCTGCCGCCGCTCGAGCACAAGGACGTGCATGCCATGGTGTACGACA
TCATGAACGACGGCCAGCGCAAGCAGTACGAGGAAGTCATGGAGGTCGACTTCTCGTTCTCGATTCCGGGCCTGGCGCGT
TTCCGCGTCAACGCCTTCAACCAGGAGCGTGGCGCTGCCGCTGTGCTGCGTACCATTCCGTCGAAGATCCTGACGCTGGA
AGAACTGAACGCCCCGAAGATCTTCGGCGACCTGGCCCTCAAGCCGCGCGGCCTGGTGCTGGTCACGGGCCCTACCGGCT
CGGGCAAGTCGACGACGCTGGCGGCGATGGTCAACCACCTCAACGAAAACGAATACGGCCACATCCTCACCATCGAGGAC
CCGATCGAATTCGTGCACGAGTCGAAGAAGTGCCTGATCAACCAGCGCGAAGTCGGCCCGCACACGTTGTCGTTCTCGAA
TTCCCTGCGCTCGGCGCTGCGCGAAGACCCCGACGCCATCCTGGTCGGCGAATTGCGCGACCTCGAGACCATCCGTCTCG
CCTTGTCGGCGGCGGAAACCGGCCACCTCGTCTTCGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
GTCGACGTCTTCCCGGCCGAGGAAAAAGAGATGGTCCGTGCGATGCTGTCGGAGTCGCTGCAGGCCGTCATTTCGCAGAC
CCTGCTGAAAACCAAGGACGGCTCGGGCCGCGTGGCCGCGCACGAGATCATGATCGGCACGCCGGCAATCCGCAACCTGA
TCCGCGAAGCGAAGATCGCGCAGATGTACTCGGCAATCCAGACCGGCAGCAATACGGGCATGCAGACGCTGGACCAGAAC
CTGACCGACCTGGTACGCCGTAACATCATTTCGAGCTCGGCTGCCCGCGCAGCGGCTAAAATCCCGGAAAACTTCCCGGG
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G5ZB84

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.681

99.424

0.772
  pilT Acinetobacter nosocomialis M2

77.391

99.424

0.769
  pilT Acinetobacter baumannii D1279779

77.391

99.424

0.769
  pilT Acinetobacter baumannii strain A118

77.391

99.424

0.769
  pilT Pseudomonas stutzeri DSM 10701

75.652

99.424

0.752
  pilT Acinetobacter baylyi ADP1

75.072

99.424

0.746
  pilT Legionella pneumophila strain Lp02

74.631

97.695

0.729
  pilT Legionella pneumophila strain ERS1305867

74.631

97.695

0.729
  pilT Neisseria gonorrhoeae MS11

69.565

99.424

0.692
  pilT Neisseria meningitidis 8013

69.565

99.424

0.692
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.656

97.118

0.657
  pilT Vibrio cholerae strain A1552

67.656

97.118

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

99.424

0.513
  pilU Pseudomonas stutzeri DSM 10701

42.388

96.542

0.409
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Vibrio cholerae strain A1552

41.593

97.695

0.406