Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   G4G31_RS08530 Genome accession   NZ_CP050451
Coordinates   1846388..1847524 (-) Length   378 a.a.
NCBI ID   WP_182991054.1    Uniprot ID   A0A7G5ZB83
Organism   Massilia sp. Se16.2.3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1841388..1852524
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G4G31_RS08505 (G4G31_08510) - 1841524..1842264 (+) 741 WP_229425461.1 S9 family peptidase -
  G4G31_RS27865 (G4G31_08515) - 1842275..1843963 (-) 1689 WP_182991050.1 methyl-accepting chemotaxis protein -
  G4G31_RS08515 (G4G31_08520) - 1843997..1844464 (-) 468 WP_182991051.1 chemotaxis protein CheW -
  G4G31_RS08520 (G4G31_08525) - 1844726..1845712 (-) 987 WP_182991052.1 MerR family transcriptional regulator -
  G4G31_RS08525 (G4G31_08530) - 1845806..1846297 (-) 492 WP_182991053.1 glutathione peroxidase -
  G4G31_RS08530 (G4G31_08535) pilU 1846388..1847524 (-) 1137 WP_182991054.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  G4G31_RS08535 (G4G31_08540) pilT 1847612..1848655 (-) 1044 WP_182991055.1 type IV pilus twitching motility protein PilT Machinery gene
  G4G31_RS08540 (G4G31_08545) - 1848747..1849447 (+) 701 Protein_1791 YggS family pyridoxal phosphate-dependent enzyme -
  G4G31_RS08545 (G4G31_08550) proC 1849462..1850277 (+) 816 WP_182991056.1 pyrroline-5-carboxylate reductase -
  G4G31_RS08550 (G4G31_08555) - 1850598..1850894 (-) 297 WP_182991057.1 YqjK family protein -
  G4G31_RS08555 (G4G31_08560) - 1850887..1851264 (-) 378 WP_182991058.1 phage holin family protein -
  G4G31_RS08560 (G4G31_08565) - 1851278..1851595 (-) 318 WP_182991059.1 YqjD family protein -
  G4G31_RS08565 (G4G31_08570) ubiA 1851666..1852520 (-) 855 WP_182991060.1 4-hydroxybenzoate octaprenyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42202.61 Da        Isoelectric Point: 6.5794

>NTDB_id=432831 G4G31_RS08530 WP_182991054.1 1846388..1847524(-) (pilU) [Massilia sp. Se16.2.3]
MERDQASKFMFDLLRLMVSKGGSDLFITAGFPPAIKIDGKMTPVSNQPLTAAHTSDLARSIMNDKQTAGFELTREANFAI
SPGDLGRFRVSAFVQMSNVGMVLRTITTQIPKLEDLELPPVLKDVIMTKRGLVIMVGATGSGKSTTLAAMVGYRNENSYG
HIITIEDPVEFVHPHRNCVITQREVGVDTDSFEAALKNSLRQAPDVIQIGEIRDRETMEHAIAFAETGHLCLATLHANSA
NQALDRIINFFPEERRQQLLMDLSLNMRGLISQRLIPRKEDKGRVVAMEIMLNTPLISDLIFKGDVHELKEIMKKSRELG
MQTFDQALFDLHEADKISYEDALRNADSVNDLRLTIKLNSKHMKNRDFSAGTEHLGLV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=432831 G4G31_RS08530 WP_182991054.1 1846388..1847524(-) (pilU) [Massilia sp. Se16.2.3]
ATGGAACGCGACCAGGCCTCAAAATTCATGTTCGACCTGCTGCGCCTGATGGTAAGCAAGGGCGGCTCCGACCTGTTCAT
CACGGCCGGCTTCCCGCCCGCGATCAAGATCGACGGCAAGATGACGCCGGTGTCGAACCAGCCGCTGACGGCCGCCCACA
CGTCCGACCTGGCGCGTTCGATCATGAACGACAAGCAGACCGCCGGCTTCGAGCTCACGCGCGAAGCGAACTTCGCGATC
AGCCCGGGCGACCTGGGACGCTTCCGCGTGTCCGCCTTTGTCCAGATGAGCAACGTCGGCATGGTGCTGCGTACGATCAC
CACCCAGATCCCGAAGCTCGAGGACCTGGAATTGCCGCCCGTCCTGAAGGACGTGATCATGACCAAGCGCGGCCTCGTGA
TCATGGTCGGCGCCACCGGCTCGGGCAAGTCGACGACGCTGGCGGCCATGGTCGGCTACCGCAACGAGAACAGCTACGGC
CACATCATCACCATCGAGGATCCGGTCGAATTCGTCCACCCGCACAGGAACTGCGTGATCACCCAGCGCGAAGTGGGCGT
GGACACCGACTCGTTCGAGGCGGCGCTGAAGAACTCCCTGCGCCAGGCGCCGGACGTGATCCAGATCGGCGAGATCCGCG
ACCGCGAAACGATGGAACACGCGATCGCCTTTGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTCGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGCCAGCAGCTCCTGATGGACCTGTCGCTGAACAT
GCGCGGCCTGATTTCGCAGCGCCTCATCCCGCGCAAGGAGGACAAGGGCCGCGTGGTGGCGATGGAGATCATGCTCAACA
CGCCCTTGATTTCGGATTTGATCTTCAAGGGCGACGTCCACGAGCTCAAGGAAATCATGAAGAAGTCGCGCGAGCTGGGC
ATGCAGACCTTCGACCAGGCCCTGTTCGACCTGCACGAGGCCGACAAGATCAGCTATGAAGACGCGCTGCGCAATGCCGA
CTCGGTGAACGACCTGCGCCTCACCATCAAGCTCAACAGCAAGCACATGAAGAACCGCGATTTCTCGGCCGGAACCGAGC
ACCTCGGTTTGGTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G5ZB83

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.47

92.328

0.595
  pilU Acinetobacter baylyi ADP1

58.333

95.238

0.556
  pilU Vibrio cholerae strain A1552

55.899

94.18

0.526
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.776

88.624

0.397
  pilT Pseudomonas aeruginosa PAK

43.582

88.624

0.386
  pilT Acinetobacter baumannii D1279779

43.202

87.566

0.378
  pilT Acinetobacter nosocomialis M2

43.202

87.566

0.378
  pilT Acinetobacter baumannii strain A118

43.202

87.566

0.378
  pilT Legionella pneumophila strain Lp02

42.9

87.566

0.376
  pilT Legionella pneumophila strain ERS1305867

42.9

87.566

0.376
  pilT Acinetobacter baylyi ADP1

43.75

84.656

0.37
  pilT Pseudomonas stutzeri DSM 10701

41.791

88.624

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.444

83.333

0.37
  pilT Vibrio cholerae strain A1552

44.444

83.333

0.37