Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HBH39_RS12265 Genome accession   NZ_CP050313
Coordinates   2798902..2799939 (-) Length   345 a.a.
NCBI ID   WP_167678677.1    Uniprot ID   A0A6G9QM75
Organism   Shewanella aestuarii strain PN3F2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2793902..2804939
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HBH39_RS12235 (HBH39_12235) ruvX 2794392..2794814 (-) 423 WP_167678665.1 Holliday junction resolvase RuvX -
  HBH39_RS12240 (HBH39_12240) - 2794859..2795419 (-) 561 WP_167678667.1 YqgE/AlgH family protein -
  HBH39_RS12245 (HBH39_12245) yciH 2795487..2795816 (-) 330 WP_167678669.1 stress response translation initiation inhibitor YciH -
  HBH39_RS12250 (HBH39_12250) hemH 2795978..2796991 (-) 1014 WP_167678671.1 ferrochelatase -
  HBH39_RS12255 (HBH39_12255) - 2797038..2797559 (-) 522 WP_244325800.1 glutathione peroxidase -
  HBH39_RS12260 (HBH39_12260) pilU 2797777..2798889 (-) 1113 WP_167678675.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HBH39_RS12265 (HBH39_12265) pilT 2798902..2799939 (-) 1038 WP_167678677.1 type IV pilus twitching motility protein PilT Machinery gene
  HBH39_RS12270 (HBH39_12270) - 2799967..2800659 (+) 693 WP_167680069.1 YggS family pyridoxal phosphate-dependent enzyme -
  HBH39_RS12275 (HBH39_12275) proC 2800757..2801575 (+) 819 WP_167678679.1 pyrroline-5-carboxylate reductase -
  HBH39_RS12280 (HBH39_12280) - 2801680..2802228 (+) 549 WP_167678680.1 YggT family protein -
  HBH39_RS12285 (HBH39_12285) - 2802270..2802581 (+) 312 WP_167680070.1 DUF167 family protein -
  HBH39_RS12290 (HBH39_12290) - 2802608..2803054 (+) 447 WP_167678682.1 DUF4426 domain-containing protein -
  HBH39_RS12295 (HBH39_12295) rdgB 2803256..2803882 (+) 627 WP_167678684.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38298.93 Da        Isoelectric Point: 6.9097

>NTDB_id=431392 HBH39_RS12265 WP_167678677.1 2798902..2799939(-) (pilT) [Shewanella aestuarii strain PN3F2]
MEITELLAFSVKHKASDLHLSAGVSPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDFEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAGAVFRTIPSEILSLEQLGAPEIFKKIASFPRGLVLVTGPTGSGKSTTLAAMVDYVNENRHEHILTIED
PIEFVHQNKQCLINQREVHKHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPEGEKSMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLDQCL
QNLVNRGLISREDAMSKSSNKQANF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=431392 HBH39_RS12265 WP_167678677.1 2798902..2799939(-) (pilT) [Shewanella aestuarii strain PN3F2]
ATGGAAATCACTGAATTACTGGCCTTTAGTGTAAAGCATAAAGCTTCAGATCTACATCTTTCTGCAGGTGTATCACCCAT
GATCCGTGTTGACGGTGAAGTCAGAAAAATTAATTTACCCGCGTTAGATCATCAAGGCGTACATAGCTTAGTGTACGATA
TTATGAATGATAAACAGCGTAAAGATTTTGAAGAACATCTTGAAATCGACTTCTCATTTGAAGTGCCCAATTTAGCACGT
TTTCGTGTGAACGCATTCAACCAGTCACGTGGTGCCGGTGCAGTATTCCGTACGATTCCATCTGAAATTTTATCGCTTGA
GCAGCTTGGTGCGCCTGAAATCTTCAAAAAAATCGCCAGTTTTCCGCGTGGGTTAGTGTTAGTTACCGGGCCGACAGGTT
CGGGTAAGTCAACAACGCTGGCGGCAATGGTCGATTACGTCAACGAAAACCGACACGAACATATTTTAACCATTGAAGAC
CCTATCGAATTCGTTCACCAAAATAAGCAATGTTTGATTAACCAGCGTGAAGTACATAAACATACCCACAGCTTTAATGC
CGCGCTTCGAAGCGCACTACGTGAAGACCCCGACGTTATTCTGGTGGGTGAGATGCGTGACTTAGAAACCATTCGCTTAG
CCATGACAGCCGCAGAAACAGGTCACTTAGTATTTGGTACCTTGCATACCACCTCAGCTGCAAAAACCATTGACCGTGTG
GTTGACGTATTCCCTGAAGGTGAAAAGAGTATGGTGCGTACCATGTTATCTGAATCATTACAGGCCGTTATTTCACAAAC
CCTGATTAAAAAAATTGGTGGCGGCCGTGTTGCTGCACATGAAATCATGATGGGAACCCCAGCAATTCGTAACCTTATTC
GTGAAGATAAAGTCGCGCAAATGTATTCAGCTATTCAAACTGGTATGGCACACGGCATGCAGACGCTTGATCAATGTTTA
CAAAATTTGGTTAACCGTGGATTAATTTCTCGTGAAGATGCAATGTCAAAAAGCTCTAATAAGCAAGCTAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G9QM75

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.009

99.42

0.786
  pilT Acinetobacter baumannii D1279779

79.009

99.42

0.786
  pilT Acinetobacter baumannii strain A118

79.009

99.42

0.786
  pilT Pseudomonas aeruginosa PAK

79.586

97.971

0.78
  pilT Pseudomonas stutzeri DSM 10701

77.971

100

0.78
  pilT Acinetobacter baylyi ADP1

79.091

95.652

0.757
  pilT Legionella pneumophila strain Lp02

75.953

98.841

0.751
  pilT Legionella pneumophila strain ERS1305867

75.953

98.841

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.404

97.681

0.707
  pilT Vibrio cholerae strain A1552

72.404

97.681

0.707
  pilT Neisseria meningitidis 8013

68.895

99.71

0.687
  pilT Neisseria gonorrhoeae MS11

68.605

99.71

0.684
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.442

98.261

0.496
  pilU Pseudomonas stutzeri DSM 10701

42.274

99.42

0.42
  pilU Vibrio cholerae strain A1552

42.56

97.391

0.414
  pilU Acinetobacter baylyi ADP1

40.95

97.681

0.4