Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HBH39_RS12260 Genome accession   NZ_CP050313
Coordinates   2797777..2798889 (-) Length   370 a.a.
NCBI ID   WP_167678675.1    Uniprot ID   A0A6G9QKQ9
Organism   Shewanella aestuarii strain PN3F2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2792777..2803889
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HBH39_RS12230 (HBH39_12230) - 2793217..2794092 (-) 876 WP_167678663.1 HAD-IIB family hydrolase -
  HBH39_RS12235 (HBH39_12235) ruvX 2794392..2794814 (-) 423 WP_167678665.1 Holliday junction resolvase RuvX -
  HBH39_RS12240 (HBH39_12240) - 2794859..2795419 (-) 561 WP_167678667.1 YqgE/AlgH family protein -
  HBH39_RS12245 (HBH39_12245) yciH 2795487..2795816 (-) 330 WP_167678669.1 stress response translation initiation inhibitor YciH -
  HBH39_RS12250 (HBH39_12250) hemH 2795978..2796991 (-) 1014 WP_167678671.1 ferrochelatase -
  HBH39_RS12255 (HBH39_12255) - 2797038..2797559 (-) 522 WP_244325800.1 glutathione peroxidase -
  HBH39_RS12260 (HBH39_12260) pilU 2797777..2798889 (-) 1113 WP_167678675.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HBH39_RS12265 (HBH39_12265) pilT 2798902..2799939 (-) 1038 WP_167678677.1 type IV pilus twitching motility protein PilT Machinery gene
  HBH39_RS12270 (HBH39_12270) - 2799967..2800659 (+) 693 WP_167680069.1 YggS family pyridoxal phosphate-dependent enzyme -
  HBH39_RS12275 (HBH39_12275) proC 2800757..2801575 (+) 819 WP_167678679.1 pyrroline-5-carboxylate reductase -
  HBH39_RS12280 (HBH39_12280) - 2801680..2802228 (+) 549 WP_167678680.1 YggT family protein -
  HBH39_RS12285 (HBH39_12285) - 2802270..2802581 (+) 312 WP_167680070.1 DUF167 family protein -
  HBH39_RS12290 (HBH39_12290) - 2802608..2803054 (+) 447 WP_167678682.1 DUF4426 domain-containing protein -
  HBH39_RS12295 (HBH39_12295) rdgB 2803256..2803882 (+) 627 WP_167678684.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41047.13 Da        Isoelectric Point: 6.3733

>NTDB_id=431391 HBH39_RS12260 WP_167678675.1 2797777..2798889(-) (pilU) [Shewanella aestuarii strain PN3F2]
MDVRPFLKIMVDKKASDLFITAGFPPSAKIDGELRPLSENRFTPEQSLEFVESLMTDIQKKEFHETSECNFAFAAKELGR
FRVSAFWQRESAGCVMRRIETRIPEVEELKLPPILKDLVMSKRGLMIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHNHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCLATLHANNANQALDRI
MHLVPESKHNQLLFDLSLNLRGIVAQQLVPKSDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLALYQEGEISYADALHHADSPNDLRLMIKLKSSDTSTAGSMEGVTLDLG

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=431391 HBH39_RS12260 WP_167678675.1 2797777..2798889(-) (pilU) [Shewanella aestuarii strain PN3F2]
ATGGATGTGCGTCCGTTTTTAAAAATAATGGTGGATAAAAAGGCGTCTGATTTATTTATCACCGCAGGTTTCCCGCCTAG
TGCCAAAATTGATGGTGAGCTGCGGCCATTATCTGAAAATCGTTTTACACCTGAACAATCATTAGAGTTTGTTGAGTCAC
TTATGACCGACATTCAAAAAAAAGAATTTCATGAAACCAGTGAGTGTAACTTTGCATTTGCGGCCAAAGAGTTGGGTCGA
TTCCGTGTGAGTGCATTTTGGCAACGTGAATCGGCTGGCTGTGTCATGCGTCGTATCGAAACCCGTATTCCAGAAGTTGA
AGAGCTAAAACTTCCGCCAATTCTTAAAGACTTGGTGATGAGTAAGCGCGGGTTGATGATCATGGTCGGTGGTACCGGTA
CAGGTAAGTCAACCTCATTGGCAGCGCTGGTGGGTTATCGCAACGCCCATGCTCGCGGCCATATTTTGACCATCGAAGAC
CCGGTTGAATTTGTTCATAATCACCGTAAAAGTATTATTACCCAACGTGAAGTCGGTATCGATACTGAATCATTTGATGC
AGCACTGAAAAGCTCGCTACGCCAAGCGCCTGATGTCATCTTAATTGGTGAGATCCGTACCCAAGAAACCATGGAGTTTG
CCTTATCTTTTGCTGAAACTGGCCACCTTTGCTTAGCAACATTGCACGCCAACAACGCTAACCAAGCATTAGACCGTATT
ATGCATTTGGTGCCAGAAAGCAAACATAATCAATTATTGTTCGACTTATCGTTAAACTTACGCGGTATTGTGGCGCAGCA
ATTAGTACCTAAATCAGATGGAACCGGACGACGCGCTGCAATTGAGGTATTAATTAATACCCCTCGTGTTGCCAGTTTGA
TTGCTAAAAATGAACTGCATTTACTCAAAGAAACCATGGCCAAATCCAATGAGCAAGGTATGCAGACATTTGACCAAGCA
CTCTTGGCACTCTATCAAGAAGGTGAAATTAGTTATGCCGATGCACTGCATCATGCTGATTCACCAAATGATCTACGCTT
AATGATCAAGCTAAAAAGCAGTGATACCTCAACTGCAGGCTCGATGGAAGGTGTCACTTTAGATTTAGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G9QKQ9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.725

100

0.619
  pilU Vibrio cholerae strain A1552

57.995

99.73

0.578
  pilU Acinetobacter baylyi ADP1

59.943

95.135

0.57
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.03

89.189

0.384
  pilT Vibrio cholerae strain A1552

43.03

89.189

0.384
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.87

93.243

0.381
  pilT Legionella pneumophila strain ERS1305867

41.692

89.459

0.373
  pilT Legionella pneumophila strain Lp02

41.692

89.459

0.373
  pilT Acinetobacter baylyi ADP1

39.766

92.432

0.368
  pilT Acinetobacter nosocomialis M2

39.474

92.432

0.365
  pilT Acinetobacter baumannii D1279779

39.474

92.432

0.365
  pilT Acinetobacter baumannii strain A118

39.474

92.432

0.365
  pilT Pseudomonas aeruginosa PAK

38.841

93.243

0.362