Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VFU_RS14845 Genome accession   NC_016602
Coordinates   3185940..3186977 (-) Length   345 a.a.
NCBI ID   WP_014205823.1    Uniprot ID   -
Organism   Vibrio furnissii NCTC 11218     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3180940..3191977
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VFU_RS14815 (vfu_A03044) - 3181126..3181827 (+) 702 WP_004724253.1 endonuclease -
  VFU_RS14820 (vfu_A03045) rsmE 3181935..3182666 (+) 732 WP_004724254.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  VFU_RS14825 (vfu_A03046) gshB 3182679..3183635 (+) 957 WP_004724255.1 glutathione synthase -
  VFU_RS14830 (vfu_A03047) - 3183674..3184237 (+) 564 WP_004724256.1 YqgE/AlgH family protein -
  VFU_RS14835 (vfu_A03048) ruvX 3184300..3184725 (+) 426 WP_014205821.1 Holliday junction resolvase RuvX -
  VFU_RS14840 (vfu_A03049) pilU 3184822..3185928 (-) 1107 WP_014205822.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VFU_RS14845 (vfu_A03050) pilT 3185940..3186977 (-) 1038 WP_014205823.1 type IV pilus twitching motility protein PilT Machinery gene
  VFU_RS14850 (vfu_A03051) - 3187003..3187710 (+) 708 WP_014205824.1 YggS family pyridoxal phosphate-dependent enzyme -
  VFU_RS14855 (vfu_A03052) proC 3187748..3188566 (+) 819 WP_014205825.1 pyrroline-5-carboxylate reductase -
  VFU_RS14860 (vfu_A03053) - 3188618..3189174 (+) 557 Protein_2891 YggT family protein -
  VFU_RS14865 (vfu_A03054) yggU 3189171..3189464 (+) 294 WP_038150700.1 DUF167 family protein YggU -
  VFU_RS14870 (vfu_A03055) - 3189522..3189953 (+) 432 WP_014205827.1 DUF4426 domain-containing protein -
  VFU_RS14875 (vfu_A03056) - 3189977..3190576 (+) 600 WP_014205828.1 XTP/dITP diphosphatase -
  VFU_RS14880 (vfu_A03057) hemW 3190576..3191748 (+) 1173 WP_014205829.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38247.82 Da        Isoelectric Point: 6.5583

>NTDB_id=42958 VFU_RS14845 WP_014205823.1 3185940..3186977(-) (pilT) [Vibrio furnissii NCTC 11218]
MDISELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHADVHRLIFEIMNDAQRSEYEEKLEVDFSFELPDIGR
FRVNAFHQSRGASAVFRTIPTTIPTLDQLHAPDIFNKIANYEKGLVLVTGPTGSGKSTTLAAMVDYINATHNKHVLTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDRETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRLGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIAQGLVDPAEVQKKIELEMSAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=42958 VFU_RS14845 WP_014205823.1 3185940..3186977(-) (pilT) [Vibrio furnissii NCTC 11218]
ATGGATATCTCTGAGTTACTGGAATTTAGTGTAAAGCATAATGCGTCAGATCTACATCTTTCTGCGGGCGTTCCTCCGAT
GGTACGTATCGATGGCGAGGTGAGAAAGCTTGGTGTGCCTGCGTTTACACACGCTGACGTGCATCGATTAATTTTTGAAA
TCATGAATGATGCCCAGCGCAGTGAGTACGAAGAAAAGCTGGAAGTGGACTTTTCCTTTGAATTACCGGATATCGGTCGT
TTTCGTGTCAACGCTTTCCATCAGTCGCGCGGCGCATCCGCAGTTTTTCGGACCATTCCAACGACGATTCCAACGCTAGA
TCAACTGCATGCGCCGGACATCTTCAACAAAATCGCCAATTACGAGAAAGGGCTAGTGTTGGTGACGGGGCCTACGGGCT
CGGGTAAATCGACCACATTGGCCGCGATGGTTGATTACATTAACGCCACCCACAACAAGCATGTGCTGACCATCGAAGAT
CCGATTGAGTTTGTGCACAGCAACAACAAATGTTTGATCAACCAACGTGAAGTGCACCGCGACACGCACAGTTTTAAAAA
TGCGCTGCGCAGTGCGCTGCGTGAAGATCCGGATGTGATTTTGGTCGGTGAGCTGCGCGATCGAGAGACCATCAGCTTAG
CGCTGACGGCGGCGGAAACCGGCCATTTGGTGTTTGGGACGCTGCACACCAGTTCGGCGGCGAAAACCATTGACCGCATC
ATTGACGTGTTTCCGGGCAGTGATAAAGACATGGTGCGTTCGATGTTGTCAGAATCGCTGCGCGCGGTGATTGCGCAGAA
ACTGCTCAAACGTCTCGGTGGTGGGCGAGTGGCGTGTCATGAAATCATGTTGGCGACACCGGCGATTCGTAACTTGATCC
GCGAAGACAAAGTCGCGCAGATGTATTCGATCATTCAGACGGGCGCGGCGCATGGCATGCAAACCATGGAACAGAACGCC
AAGCAGTTGATTGCACAAGGGCTGGTGGATCCGGCGGAAGTGCAGAAGAAAATTGAACTGGAAATGTCCGCATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

92.754

100

0.928
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

92.754

100

0.928
  pilT Acinetobacter baumannii D1279779

71.903

95.942

0.69
  pilT Acinetobacter baumannii strain A118

71.903

95.942

0.69
  pilT Acinetobacter baylyi ADP1

71.818

95.652

0.687
  pilT Acinetobacter nosocomialis M2

71.601

95.942

0.687
  pilT Pseudomonas stutzeri DSM 10701

68.546

97.681

0.67
  pilT Pseudomonas aeruginosa PAK

66.765

98.551

0.658
  pilT Legionella pneumophila strain Lp02

67.477

95.362

0.643
  pilT Legionella pneumophila strain ERS1305867

67.477

95.362

0.643
  pilT Neisseria meningitidis 8013

62.941

98.551

0.62
  pilT Neisseria gonorrhoeae MS11

62.647

98.551

0.617
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.774

92.174

0.496
  pilU Pseudomonas stutzeri DSM 10701

42.47

96.232

0.409
  pilU Vibrio cholerae strain A1552

42.769

94.203

0.403
  pilU Acinetobacter baylyi ADP1

40.938

92.754

0.38

Multiple sequence alignment