Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VFU_RS14840 Genome accession   NC_016602
Coordinates   3184822..3185928 (-) Length   368 a.a.
NCBI ID   WP_014205822.1    Uniprot ID   -
Organism   Vibrio furnissii NCTC 11218     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3179822..3190928
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VFU_RS14810 (vfu_A03043) - 3180488..3180988 (+) 501 WP_014205820.1 SprT family zinc-dependent metalloprotease -
  VFU_RS14815 (vfu_A03044) - 3181126..3181827 (+) 702 WP_004724253.1 endonuclease -
  VFU_RS14820 (vfu_A03045) rsmE 3181935..3182666 (+) 732 WP_004724254.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  VFU_RS14825 (vfu_A03046) gshB 3182679..3183635 (+) 957 WP_004724255.1 glutathione synthase -
  VFU_RS14830 (vfu_A03047) - 3183674..3184237 (+) 564 WP_004724256.1 YqgE/AlgH family protein -
  VFU_RS14835 (vfu_A03048) ruvX 3184300..3184725 (+) 426 WP_014205821.1 Holliday junction resolvase RuvX -
  VFU_RS14840 (vfu_A03049) pilU 3184822..3185928 (-) 1107 WP_014205822.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VFU_RS14845 (vfu_A03050) pilT 3185940..3186977 (-) 1038 WP_014205823.1 type IV pilus twitching motility protein PilT Machinery gene
  VFU_RS14850 (vfu_A03051) - 3187003..3187710 (+) 708 WP_014205824.1 YggS family pyridoxal phosphate-dependent enzyme -
  VFU_RS14855 (vfu_A03052) proC 3187748..3188566 (+) 819 WP_014205825.1 pyrroline-5-carboxylate reductase -
  VFU_RS14860 (vfu_A03053) - 3188618..3189174 (+) 557 Protein_2891 YggT family protein -
  VFU_RS14865 (vfu_A03054) yggU 3189171..3189464 (+) 294 WP_038150700.1 DUF167 family protein YggU -
  VFU_RS14870 (vfu_A03055) - 3189522..3189953 (+) 432 WP_014205827.1 DUF4426 domain-containing protein -
  VFU_RS14875 (vfu_A03056) - 3189977..3190576 (+) 600 WP_014205828.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41357.40 Da        Isoelectric Point: 6.5588

>NTDB_id=42957 VFU_RS14840 WP_014205822.1 3184822..3185928(-) (pilU) [Vibrio furnissii NCTC 11218]
MQLEECLQGMMTHKASDLYITVGAPVLYRVDGELRAHGDALNEAQVRTLLAAMMDDTRHADYLATREANFAIVRDLGRFR
VSAFFQRELPGAVIRRIETQIPTFDELKLPPVLKDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNMHRTGHILTVEDPI
EFVHEHQRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEVLLNSPRVSDIIRRGELHELKNTMAKSKEVGMQTFDQALY
QLVVEHKISEQDALHSADSANDLRLMLKTQRGDVTSSGSLDHVKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=42957 VFU_RS14840 WP_014205822.1 3184822..3185928(-) (pilU) [Vibrio furnissii NCTC 11218]
ATGCAACTTGAGGAGTGCCTGCAAGGCATGATGACGCATAAAGCGTCGGATCTGTATATCACTGTGGGCGCGCCCGTGTT
GTATCGGGTCGATGGTGAACTGCGCGCCCACGGCGACGCGCTGAATGAAGCGCAGGTCAGGACGCTGTTGGCCGCGATGA
TGGATGACACGCGCCATGCCGACTACTTGGCGACGCGTGAAGCCAACTTTGCCATTGTGCGTGATTTAGGGCGCTTTCGC
GTCAGCGCTTTTTTTCAGCGCGAGCTGCCCGGCGCAGTGATCCGCCGCATTGAAACGCAAATTCCCACGTTTGATGAACT
CAAACTGCCACCCGTGTTGAAAGATCTCTCGATTGCCAAACGCGGTTTGGTGTTGGTGGTGGGGGCGACTGGATCGGGGA
AATCGACCACCATGGCCGCCATGACCGGGTATCGCAATATGCATCGCACCGGGCATATTCTCACCGTTGAAGATCCGATT
GAATTTGTCCATGAACATCAGCGCTGCATCGTGACGCAGCGCGAAGTGGGATTGGATACGGAAAGCTATGAAGTGGCGCT
GAAGAACTCGCTGCGTCAGGCGCCGGACATGATTTTGATCGGCGAGATCCGCAGTCGGGAAACCATGGAATACGCGATGA
CGTTTGCCGAAACGGGGCATTTGTGCATGGCCACTTTGCACGCCAACAACGCCAACCAAGCGCTTGAACGGATTTTACAT
CTGGTGCCGAAAGAGCAGCGTGAACAATTTCTGTTTGATTTGTCGATGAACCTCAAAGGGGTTGTCGGCCAGCAACTGAT
CCGCGATAAAAACGGCCAAGGGCGTCACGGGGTGTTTGAAGTGCTGCTCAACAGCCCGCGGGTGTCGGACATCATTCGTC
GTGGCGAGCTGCATGAGTTGAAAAACACCATGGCGAAATCAAAAGAGGTCGGCATGCAGACTTTTGATCAGGCGTTGTAT
CAGTTGGTGGTTGAGCACAAGATCAGCGAGCAAGATGCGCTGCACAGTGCTGATTCGGCCAATGATCTGCGCTTGATGCT
CAAGACGCAGCGGGGCGATGTGACCAGCAGTGGGTCGCTGGATCATGTGAAAATCGACATGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(73-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

86.141

100

0.861
  pilU Pseudomonas stutzeri DSM 10701

57.143

95.109

0.543
  pilU Acinetobacter baylyi ADP1

54.416

95.38

0.519
  pilT Acinetobacter baylyi ADP1

41.642

92.663

0.386
  pilT Legionella pneumophila strain Lp02

41.593

92.12

0.383
  pilT Legionella pneumophila strain ERS1305867

41.593

92.12

0.383
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.077

88.315

0.38
  pilT Acinetobacter baumannii D1279779

41.003

92.12

0.378
  pilT Acinetobacter baumannii strain A118

41.003

92.12

0.378
  pilT Acinetobacter nosocomialis M2

41.003

92.12

0.378
  pilT Pseudomonas aeruginosa PAK

40.708

92.12

0.375
  pilT Pseudomonas stutzeri DSM 10701

39.233

92.12

0.361

Multiple sequence alignment