Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   G8A07_RS05770 Genome accession   NZ_CP049919
Coordinates   1231883..1232926 (+) Length   347 a.a.
NCBI ID   WP_195796130.1    Uniprot ID   A0A7S9C5J2
Organism   Roseateles sp. DAIF2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1226883..1237926
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G8A07_RS05750 (G8A07_05750) - 1227743..1229005 (-) 1263 WP_195796126.1 cytochrome c -
  G8A07_RS05755 (G8A07_05755) - 1229002..1229700 (-) 699 WP_195796127.1 c-type cytochrome -
  G8A07_RS05760 (G8A07_05760) - 1229745..1231169 (-) 1425 WP_195796128.1 PLP-dependent aminotransferase family protein -
  G8A07_RS05765 (G8A07_05765) - 1231166..1231855 (-) 690 WP_195796129.1 YggS family pyridoxal phosphate-dependent enzyme -
  G8A07_RS05770 (G8A07_05770) pilT 1231883..1232926 (+) 1044 WP_195796130.1 type IV pilus twitching motility protein PilT Machinery gene
  G8A07_RS05775 (G8A07_05775) pilU 1233074..1234210 (+) 1137 WP_195796131.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  G8A07_RS05780 (G8A07_05780) - 1234221..1235123 (+) 903 WP_195796132.1 NAD(P)-dependent oxidoreductase -
  G8A07_RS05785 (G8A07_05785) - 1235167..1235793 (-) 627 WP_195796133.1 BON domain-containing protein -
  G8A07_RS05790 (G8A07_05790) - 1235835..1236386 (-) 552 WP_195796134.1 SIS domain-containing protein -
  G8A07_RS05795 (G8A07_05795) - 1236410..1236799 (-) 390 WP_195796135.1 YraN family protein -
  G8A07_RS05800 (G8A07_05800) rsmI 1236792..1237706 (+) 915 WP_249937237.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38209.78 Da        Isoelectric Point: 7.1148

>NTDB_id=428680 G8A07_RS05770 WP_195796130.1 1231883..1232926(+) (pilT) [Roseateles sp. DAIF2]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVEPLEHRHVHDMVYDIMNDSQRKAYEETLECDFSFEIQGLAR
FRVNAFNQNRGAGAVFRTIPSKILTLEQLNAPKVFAELALKPRGLVLVTGPTGSGKSTTLAGMVNHLNENEYGHILTIED
PIEFVHESKKSLINQREVGPHTLSFANALRSALREDPDAVLVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
VDVFPAAEKEMVRAMVSESLVAVISQSLCKLKDGTGRVAAHEIMLGTSAIRNLIRENKVAQMYSAIQTGNSQGMQTLDQN
LTDLVRRNIISPAEARAKAKFPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=428680 G8A07_RS05770 WP_195796130.1 1231883..1232926(+) (pilT) [Roseateles sp. DAIF2]
ATGGATATCACCCAACTGCTGGCATTCTCGGTCAAGAACAAGGCCTCCGACCTGCACCTCTCCGCCGGGCTGCCGCCGAT
GATCCGGGTGCATGGCGACGTGCGGCGCATCAATGTCGAACCGCTCGAGCACCGCCATGTGCACGACATGGTGTACGACA
TCATGAACGACTCGCAGCGCAAGGCCTACGAAGAGACCCTGGAGTGCGACTTCTCGTTCGAGATCCAGGGCCTGGCGCGC
TTTCGCGTCAATGCCTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGCACGATTCCGTCCAAGATCCTGACCCTGGA
GCAGCTCAACGCGCCCAAGGTCTTCGCCGAGCTGGCCCTGAAGCCGCGCGGCCTGGTGCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACCCTGGCCGGCATGGTCAACCACCTGAACGAAAACGAGTACGGCCATATCCTCACGATCGAGGAC
CCGATCGAGTTCGTGCATGAATCGAAGAAGAGCCTGATCAACCAGCGCGAGGTCGGCCCGCACACCCTGAGCTTCGCCAA
CGCGCTGCGCTCGGCGCTGCGCGAGGATCCGGACGCGGTGCTGGTCGGCGAAATGCGCGACCTGGAGACGATCCGCCTGG
CGCTGACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACCCTGCACACCTCCAGCGCCGCCAAGACGGTGGACCGCATC
GTCGACGTGTTCCCCGCGGCAGAGAAGGAGATGGTGCGCGCGATGGTGTCGGAATCGCTGGTCGCGGTGATCTCGCAGAG
CCTGTGCAAGCTCAAGGACGGCACCGGCCGCGTCGCGGCGCACGAGATCATGCTGGGCACCTCGGCGATCCGCAACCTCA
TCCGCGAGAACAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGCAACAGCCAGGGCATGCAGACCCTGGACCAGAAC
CTGACCGACCTGGTCCGCCGCAACATCATCTCGCCGGCCGAGGCCCGCGCCAAGGCGAAGTTTCCGGAGAACTTTCCGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7S9C5J2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.942

99.424

0.755
  pilT Acinetobacter nosocomialis M2

74.783

99.424

0.744
  pilT Acinetobacter baumannii D1279779

74.783

99.424

0.744
  pilT Acinetobacter baumannii strain A118

74.783

99.424

0.744
  pilT Pseudomonas stutzeri DSM 10701

74.203

99.424

0.738
  pilT Acinetobacter baylyi ADP1

72.754

99.424

0.723
  pilT Legionella pneumophila strain Lp02

71.091

97.695

0.695
  pilT Legionella pneumophila strain ERS1305867

71.091

97.695

0.695
  pilT Neisseria meningitidis 8013

68.696

99.424

0.683
  pilT Neisseria gonorrhoeae MS11

68.406

99.424

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.568

97.406

0.648
  pilT Vibrio cholerae strain A1552

66.568

97.406

0.648
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

99.424

0.513
  pilU Acinetobacter baylyi ADP1

40.462

99.712

0.403
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398
  pilU Vibrio cholerae strain A1552

41.194

96.542

0.398