Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   G8A07_RS05775 Genome accession   NZ_CP049919
Coordinates   1233074..1234210 (+) Length   378 a.a.
NCBI ID   WP_195796131.1    Uniprot ID   -
Organism   Roseateles sp. DAIF2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1228074..1239210
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G8A07_RS05755 (G8A07_05755) - 1229002..1229700 (-) 699 WP_195796127.1 c-type cytochrome -
  G8A07_RS05760 (G8A07_05760) - 1229745..1231169 (-) 1425 WP_195796128.1 PLP-dependent aminotransferase family protein -
  G8A07_RS05765 (G8A07_05765) - 1231166..1231855 (-) 690 WP_195796129.1 YggS family pyridoxal phosphate-dependent enzyme -
  G8A07_RS05770 (G8A07_05770) pilT 1231883..1232926 (+) 1044 WP_195796130.1 type IV pilus twitching motility protein PilT Machinery gene
  G8A07_RS05775 (G8A07_05775) pilU 1233074..1234210 (+) 1137 WP_195796131.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  G8A07_RS05780 (G8A07_05780) - 1234221..1235123 (+) 903 WP_195796132.1 NAD(P)-dependent oxidoreductase -
  G8A07_RS05785 (G8A07_05785) - 1235167..1235793 (-) 627 WP_195796133.1 BON domain-containing protein -
  G8A07_RS05790 (G8A07_05790) - 1235835..1236386 (-) 552 WP_195796134.1 SIS domain-containing protein -
  G8A07_RS05795 (G8A07_05795) - 1236410..1236799 (-) 390 WP_195796135.1 YraN family protein -
  G8A07_RS05800 (G8A07_05800) rsmI 1236792..1237706 (+) 915 WP_249937237.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  G8A07_RS05805 (G8A07_05805) tldD 1237714..1239177 (+) 1464 WP_195796136.1 metalloprotease TldD -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42000.09 Da        Isoelectric Point: 6.6645

>NTDB_id=428681 G8A07_RS05775 WP_195796131.1 1233074..1234210(+) (pilU) [Roseateles sp. DAIF2]
MERDQASKFINDLLRLMISRRGSDLFLTADFPPAIKVDGKVTKVSPQPLTGSHTLQLARSIMNDKQAAEFERSKECNFAI
SPAGVGRFRANAFLQQGQVGLVLRTIPATLPSIAQLDLPPILNDVVQAKRGLVIVVGATGSGKSTTLAAMIDHRNETTHG
HIITIEDPIEFVHPHKNCIVTQREVGIDTDNWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLVSQRLLPRQEGKGRIAAVEILLNTPLISDMIFKGEVGEIKEVMKRSREQG
MQTFDQALFDLYENGKVNYEDALRNADSVNDLRLQIKLNSERARQGDLSSGTEHLTIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=428681 G8A07_RS05775 WP_195796131.1 1233074..1234210(+) (pilU) [Roseateles sp. DAIF2]
ATGGAACGCGATCAGGCCTCGAAATTCATCAATGATCTGCTGCGCCTGATGATCTCGCGCCGCGGCTCGGACCTGTTCCT
GACCGCGGACTTCCCGCCGGCGATCAAGGTCGACGGCAAGGTCACCAAGGTCTCGCCGCAGCCGCTGACCGGCAGCCACA
CCCTGCAGCTGGCGCGTTCGATCATGAACGACAAGCAGGCCGCCGAGTTCGAGCGCAGCAAGGAGTGCAATTTCGCGATC
TCGCCGGCCGGTGTCGGGCGCTTCCGCGCCAATGCCTTCCTGCAGCAGGGCCAGGTCGGCCTGGTGCTGCGCACGATTCC
CGCCACCCTGCCCAGCATCGCCCAGCTGGATCTGCCGCCGATCCTGAACGATGTGGTGCAGGCCAAGCGCGGGCTGGTGA
TCGTGGTCGGCGCCACCGGCTCGGGCAAGAGCACGACCCTGGCGGCGATGATCGACCATCGCAACGAGACCACCCACGGC
CACATCATCACGATCGAGGATCCGATCGAGTTCGTGCATCCGCACAAGAACTGCATCGTGACCCAGCGCGAGGTCGGCAT
CGACACCGACAACTGGGAGGCGGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACCATGGAGCATGCGGTGGCCTTCGCCGAGACCGGCCACCTCTGCATGGCGACCCTGCATGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCATCAACTTCTTCCCCGAGGAGCGCCGCGCCCAGCTGCTGATGGACCTGTCGCTGAACCT
GCGCTCCCTGGTCAGCCAGCGCCTGCTGCCGCGCCAGGAGGGCAAGGGCCGCATCGCCGCGGTGGAGATCCTGCTGAACA
CGCCGCTGATCTCGGACATGATCTTCAAGGGCGAGGTCGGCGAGATCAAGGAAGTGATGAAGCGATCGCGCGAGCAGGGC
ATGCAGACCTTCGACCAGGCCTTGTTCGACCTCTACGAGAACGGCAAGGTCAACTACGAGGACGCGCTGCGCAATGCCGA
CTCGGTCAACGACCTGCGCCTGCAGATCAAGCTCAACAGCGAGCGCGCGCGCCAGGGCGATTTGTCCTCGGGCACCGAGC
ATCTGACGATCGTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.202

96.825

0.593
  pilU Acinetobacter baylyi ADP1

58.924

93.386

0.55
  pilU Vibrio cholerae strain A1552

52.893

96.032

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.774

89.153

0.426
  pilT Pseudomonas aeruginosa PAK

44.214

89.153

0.394
  pilT Acinetobacter baylyi ADP1

43.544

88.095

0.384
  pilT Vibrio cholerae strain A1552

43.373

87.831

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.373

87.831

0.381
  pilT Legionella pneumophila strain ERS1305867

44.41

85.185

0.378
  pilT Legionella pneumophila strain Lp02

44.41

85.185

0.378
  pilT Pseudomonas stutzeri DSM 10701

42.433

89.153

0.378
  pilT Acinetobacter nosocomialis M2

42.643

88.095

0.376
  pilT Acinetobacter baumannii D1279779

42.643

88.095

0.376
  pilT Acinetobacter baumannii strain A118

42.643

88.095

0.376