Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   G7083_RS04120 Genome accession   NZ_CP049913
Coordinates   883814..884851 (-) Length   345 a.a.
NCBI ID   WP_166013712.1    Uniprot ID   -
Organism   Vibrio sp. HDW18     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 878814..889851
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G7083_RS04095 (G7083_04095) - 879171..879734 (+) 564 WP_166013707.1 YqgE/AlgH family protein -
  G7083_RS04100 (G7083_04100) ruvX 879930..880352 (+) 423 WP_166013708.1 Holliday junction resolvase RuvX -
  G7083_RS04105 (G7083_04105) tyrS 880410..881696 (-) 1287 WP_166013709.1 tyrosine--tRNA ligase -
  G7083_RS04110 (G7083_04110) - 881818..882615 (-) 798 WP_166013710.1 helix-turn-helix transcriptional regulator -
  G7083_RS04115 (G7083_04115) pilU 882694..883800 (-) 1107 WP_166013711.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  G7083_RS04120 (G7083_04120) pilT 883814..884851 (-) 1038 WP_166013712.1 type IV pilus twitching motility protein PilT Machinery gene
  G7083_RS04125 (G7083_04125) - 884877..885584 (+) 708 WP_166013713.1 YggS family pyridoxal phosphate-dependent enzyme -
  G7083_RS04130 (G7083_04130) proC 885641..886459 (+) 819 WP_166013714.1 pyrroline-5-carboxylate reductase -
  G7083_RS04135 (G7083_04135) - 886516..887073 (+) 558 WP_166013715.1 YggT family protein -
  G7083_RS04140 (G7083_04140) yggU 887073..887363 (+) 291 WP_166013716.1 DUF167 family protein YggU -
  G7083_RS04145 (G7083_04145) - 887448..888050 (+) 603 WP_166013717.1 XTP/dITP diphosphatase -
  G7083_RS04150 (G7083_04150) hemW 888044..889219 (+) 1176 WP_166013718.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38240.71 Da        Isoelectric Point: 6.5041

>NTDB_id=428594 G7083_RS04120 WP_166013712.1 883814..884851(-) (pilT) [Vibrio sp. HDW18]
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFSHSEVHRLVFEIMNDAQRSEYEEKLEVDFSFELPNVGR
FRVNAFHQARGASAVFRTIPSVIPTLEQLEAPEIFSKIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQNLLKRVGGGRIACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLIARGVVDAEQAQHKIEMDLKGF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=428594 G7083_RS04120 WP_166013712.1 883814..884851(-) (pilT) [Vibrio sp. HDW18]
ATGGATATCGCCGAGTTACTGGAGTTCAGTGTAAAGCATAACGCATCCGATCTACACCTTTCCGCAGGTGTTCCACCTAT
GGTACGGATTGATGGCGAAGTTAGGAAGCTTGGCGTGCCGGCTTTTAGTCATTCTGAGGTGCATCGCTTGGTGTTTGAAA
TTATGAATGATGCCCAGCGCAGTGAGTATGAAGAGAAACTGGAAGTCGATTTCTCCTTTGAGCTGCCGAATGTGGGTCGC
TTTCGGGTCAACGCATTTCATCAAGCGCGTGGCGCTTCGGCGGTATTTCGTACCATCCCAAGCGTGATCCCCACCTTAGA
GCAGCTTGAGGCCCCAGAAATTTTCAGCAAAATCGCCAACTATGAAAAAGGCTTAGTCTTGGTGACTGGGCCTACTGGAT
CGGGCAAATCTACCACTTTGGCGGCAATGGTCGATTACGTCAATGCTCACCATAACAAGCATATTTTAACCATTGAAGAT
CCGATTGAGTTTGTGCACAGCAACAACAAATGTTTGATTAATCAACGTGAAGTTCATCGTGATACCCACAGTTTTAAAAA
TGCGCTGCGCTCAGCGCTGCGTGAAGATCCGGATGTGATTTTAGTCGGAGAGCTGCGCGATCAAGAAACCATCAGTTTGG
CGCTCACCGCAGCAGAAACTGGGCACTTGGTATTTGGTACTTTGCACACCAGCTCGGCGGCCAAAACCATTGACCGGATC
ATTGATGTTTTCCCAGGCAGTGATAAAGATATGGTGCGCTCAATGCTCTCGGAATCGCTGCGTGCGGTCATTGCACAGAA
TCTGCTCAAACGAGTCGGCGGTGGGCGGATCGCTTGTCATGAGATCATGCTGGCCACCCCTGCGATCCGCAATTTGATCC
GCGAAGATAAAGTGGCGCAGATGTATTCGATTATTCAAACTGGCGCGGCACATGGTATGCAAACCATGGAGCAAAATGCC
AGACAACTGATCGCCCGAGGTGTGGTCGATGCTGAGCAAGCACAGCACAAAATCGAAATGGATTTAAAAGGATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

95.362

100

0.954
  pilT Vibrio cholerae strain A1552

95.362

100

0.954
  pilT Acinetobacter baumannii D1279779

72.239

97.101

0.701
  pilT Acinetobacter baumannii strain A118

72.239

97.101

0.701
  pilT Acinetobacter nosocomialis M2

71.94

97.101

0.699
  pilT Acinetobacter baylyi ADP1

71.94

97.101

0.699
  pilT Pseudomonas stutzeri DSM 10701

69.139

97.681

0.675
  pilT Pseudomonas aeruginosa PAK

67.353

98.551

0.664
  pilT Legionella pneumophila strain Lp02

66.766

96.812

0.646
  pilT Legionella pneumophila strain ERS1305867

66.766

96.812

0.646
  pilT Neisseria meningitidis 8013

64.118

98.551

0.632
  pilT Neisseria gonorrhoeae MS11

63.824

98.551

0.629
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.516

92.174

0.484
  pilU Vibrio cholerae strain A1552

41.84

97.681

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.101

0.4
  pilU Acinetobacter baylyi ADP1

40

92.754

0.371