Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   G7083_RS04115 Genome accession   NZ_CP049913
Coordinates   882694..883800 (-) Length   368 a.a.
NCBI ID   WP_166013711.1    Uniprot ID   A0A6G8D3G2
Organism   Vibrio sp. HDW18     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 877694..888800
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G7083_RS04090 (G7083_04090) gshB 878180..879136 (+) 957 WP_166013706.1 glutathione synthase -
  G7083_RS04095 (G7083_04095) - 879171..879734 (+) 564 WP_166013707.1 YqgE/AlgH family protein -
  G7083_RS04100 (G7083_04100) ruvX 879930..880352 (+) 423 WP_166013708.1 Holliday junction resolvase RuvX -
  G7083_RS04105 (G7083_04105) tyrS 880410..881696 (-) 1287 WP_166013709.1 tyrosine--tRNA ligase -
  G7083_RS04110 (G7083_04110) - 881818..882615 (-) 798 WP_166013710.1 helix-turn-helix transcriptional regulator -
  G7083_RS04115 (G7083_04115) pilU 882694..883800 (-) 1107 WP_166013711.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  G7083_RS04120 (G7083_04120) pilT 883814..884851 (-) 1038 WP_166013712.1 type IV pilus twitching motility protein PilT Machinery gene
  G7083_RS04125 (G7083_04125) - 884877..885584 (+) 708 WP_166013713.1 YggS family pyridoxal phosphate-dependent enzyme -
  G7083_RS04130 (G7083_04130) proC 885641..886459 (+) 819 WP_166013714.1 pyrroline-5-carboxylate reductase -
  G7083_RS04135 (G7083_04135) - 886516..887073 (+) 558 WP_166013715.1 YggT family protein -
  G7083_RS04140 (G7083_04140) yggU 887073..887363 (+) 291 WP_166013716.1 DUF167 family protein YggU -
  G7083_RS04145 (G7083_04145) - 887448..888050 (+) 603 WP_166013717.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41438.38 Da        Isoelectric Point: 6.6211

>NTDB_id=428593 G7083_RS04115 WP_166013711.1 882694..883800(-) (pilU) [Vibrio sp. HDW18]
MDLNQYLDGMLTHKASDLYITVGAPMLYRVDGELRAQGEVLNVAQVTTLLNAMMDDVRQAEFKQTREANFAVVRDSGRFR
VSAFFQRELPGAVIRRIETQIPTFEELNLPAVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHQHQRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIGQQLLRDKHGQGRHGVFEILLNSPRIADLIRRGELHELKTTMARSQEIGMQTFDQALY
QLVVDDKINEQDALHSADSANDLRLMLKTKRGTHYSSGALHNVQIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=428593 G7083_RS04115 WP_166013711.1 882694..883800(-) (pilU) [Vibrio sp. HDW18]
ATGGATTTAAATCAATATCTGGATGGTATGCTGACGCATAAAGCATCGGATCTGTACATTACCGTGGGCGCACCGATGTT
ATATCGGGTTGATGGCGAACTGCGCGCACAAGGTGAAGTGCTCAATGTGGCACAGGTGACCACGTTGCTGAATGCGATGA
TGGATGACGTGCGGCAAGCCGAATTTAAGCAGACTCGTGAAGCGAACTTTGCCGTAGTACGCGACAGTGGCCGCTTTCGA
GTCAGTGCCTTTTTTCAGCGTGAGCTGCCGGGGGCGGTGATCCGCCGTATTGAAACTCAGATCCCGACTTTTGAAGAGCT
TAACTTGCCGGCGGTGCTACAAAATTTGGCGATTGCCAAACGCGGTTTAGTGCTGGTGGTTGGGGCCACGGGCTCGGGTA
AATCGACCACTATGGCGGCGATGACCGGTTATCGGAATCAGCATCGTACTGGGCACATTTTAACCGTGGAAGATCCGATT
GAGTTTGTGCATCAGCATCAGCGCTGCATCGTCACTCAACGTGAAGTGGGGCTGGATACCGAAAGTTATGAAGTGGCGCT
GAAAAACTCGCTACGCCAAGCGCCAGATATGATCTTGATTGGTGAAATCCGTAGCCGGGAGACGATGGAATACGCGATGA
CTTTTGCAGAAACTGGTCATTTGTGTATGGCCACTTTGCACGCCAACAATGCCAACCAAGCCTTAGAGCGCATTTTGCAT
TTGGTGCCTAAAGAGCAGCGCGAGCAATTTTTACTCGACTTATCGCTCAATCTCAAAGGGGTGATTGGTCAACAGTTGCT
ACGTGATAAGCACGGCCAAGGCCGACATGGGGTGTTTGAAATTCTGCTCAATAGTCCGCGCATTGCCGATTTAATTCGGC
GTGGTGAGCTGCATGAGCTGAAAACCACTATGGCGCGCTCACAAGAAATTGGTATGCAGACCTTTGATCAAGCGCTGTAT
CAATTGGTGGTGGATGACAAAATCAATGAGCAAGATGCGCTACACAGCGCTGATTCGGCCAACGATCTGCGTTTGATGCT
CAAAACCAAGCGTGGCACTCACTACTCAAGTGGTGCTCTGCACAATGTGCAAATTGATATGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8D3G2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

92.935

100

0.929
  pilU Pseudomonas stutzeri DSM 10701

57.714

95.109

0.549
  pilU Acinetobacter baylyi ADP1

54.131

95.38

0.516
  pilT Legionella pneumophila strain Lp02

42.183

92.12

0.389
  pilT Legionella pneumophila strain ERS1305867

42.183

92.12

0.389
  pilT Acinetobacter baylyi ADP1

41.935

92.663

0.389
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.484

84.239

0.383
  pilT Acinetobacter nosocomialis M2

41.003

92.12

0.378
  pilT Acinetobacter baumannii D1279779

41.003

92.12

0.378
  pilT Acinetobacter baumannii strain A118

41.003

92.12

0.378
  pilT Pseudomonas aeruginosa PAK

40.708

92.12

0.375
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.118

92.12

0.37