Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   G7047_RS20365 Genome accession   NZ_CP049910
Coordinates   4446764..4447807 (+) Length   347 a.a.
NCBI ID   WP_166309533.1    Uniprot ID   A0A6G8CUG3
Organism   Diaphorobacter sp. HDW4A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4441764..4452807
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G7047_RS20335 (G7047_20340) - 4441913..4442410 (+) 498 WP_240939203.1 hypothetical protein -
  G7047_RS20340 (G7047_20345) - 4442439..4443776 (-) 1338 WP_240939204.1 DUF445 domain-containing protein -
  G7047_RS20345 (G7047_20350) - 4443880..4444296 (-) 417 WP_166309525.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  G7047_RS20350 (G7047_20355) - 4444293..4444634 (-) 342 WP_166309527.1 hypothetical protein -
  G7047_RS20355 (G7047_20360) - 4444647..4445783 (-) 1137 WP_166309529.1 aminotransferase class V-fold PLP-dependent enzyme -
  G7047_RS20360 (G7047_20365) - 4445923..4446645 (-) 723 WP_166309531.1 YggS family pyridoxal phosphate-dependent enzyme -
  G7047_RS20365 (G7047_20370) pilT 4446764..4447807 (+) 1044 WP_166309533.1 type IV pilus twitching motility protein PilT Machinery gene
  G7047_RS20370 (G7047_20375) - 4447873..4448505 (+) 633 WP_166309535.1 cyclic nucleotide-binding domain-containing protein -
  G7047_RS20375 (G7047_20380) pilU 4448605..4449741 (+) 1137 WP_166309537.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  G7047_RS20380 (G7047_20385) - 4449803..4450717 (+) 915 WP_166309539.1 NAD(P)-dependent oxidoreductase -
  G7047_RS20385 (G7047_20390) - 4450972..4451748 (-) 777 WP_166309541.1 BON domain-containing protein -
  G7047_RS20390 (G7047_20395) - 4451748..4452341 (-) 594 WP_166309543.1 SIS domain-containing protein -
  G7047_RS20395 (G7047_20400) - 4452441..4452806 (-) 366 WP_166309545.1 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38161.92 Da        Isoelectric Point: 6.7743

>NTDB_id=428571 G7047_RS20365 WP_166309533.1 4446764..4447807(+) (pilT) [Diaphorobacter sp. HDW4A]
MDITQLLAFSVKNKASDLHLSSGLPPMIRVHGDVRRINVDALDHKTVHGMVYDIMSDSQRKTYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPKIFGDLALKPRGLVLVTGPTGSGKSTTLAAMVNYLNETEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSNGVGMQTLDQN
LTDLVRRNIISPAEARAKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=428571 G7047_RS20365 WP_166309533.1 4446764..4447807(+) (pilT) [Diaphorobacter sp. HDW4A]
GTGGACATTACCCAACTGCTGGCATTCAGCGTCAAGAACAAAGCCTCCGACTTGCACCTCTCCTCCGGCCTGCCGCCGAT
GATCCGGGTGCACGGCGATGTGCGCCGCATCAACGTGGACGCGCTCGATCACAAGACCGTGCATGGCATGGTCTACGACA
TCATGAGCGACTCGCAGCGCAAGACGTATGAAGAGTTCCTCGAGGTCGACTTCTCGTTCGAAATCGAAGGCCTCGCGCGC
TTCCGGGTGAATGCCTTCAACCAGAACCGGGGCGCTGCCGCCGTGTTCCGGACGATTCCCAGCAAGATCCTGACGCTTGA
GCAGCTGAACGCGCCCAAGATCTTCGGTGACCTCGCGCTCAAGCCGCGCGGTCTGGTGCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCCACGACGCTGGCCGCGATGGTCAACTACCTGAACGAAACCGAGTACGGCCACATCCTCACGGTGGAAGAT
CCAATCGAATTCGTGCACGAATCCAAGAAGTGCCTGATCAACCAGCGCGAAGTCGGCCCGATGACGCTGTCGTTCGCCGC
AGCGCTGAAGTCCGCGCTGCGGGAAGATCCGGACGCGATTCTGGTCGGCGAAATGCGAGATCTTGAAACCATCCGCCTCG
CGATGACCGCCGCCGAAACCGGTCACCTGGTGTTCGGCACCCTGCACACCTCGAGCGCCGCCAAGACCATCGACCGTATC
ATCGACGTGTTCCCGGCCGAAGAAAAGGAAATGGTCCGCGCGATGCTCTCCGAATCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGCGTGGCGGCGCACGAAATCATGCTGGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAAGCCAAGGTGGCGCAGATGTATTCGACCATCCAGACCAGCAACGGTGTAGGCATGCAGACGCTGGACCAGAAT
TTGACAGACTTGGTGCGACGCAACATCATCAGCCCGGCCGAGGCCCGTGCCAAGGCCAAGATTCCGGAGAATTTCCCGGG
ATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8CUG3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

76.232

99.424

0.758
  pilT Acinetobacter baumannii strain A118

76.232

99.424

0.758
  pilT Acinetobacter nosocomialis M2

76.232

99.424

0.758
  pilT Pseudomonas stutzeri DSM 10701

75.942

99.424

0.755
  pilT Pseudomonas aeruginosa PAK

75.942

99.424

0.755
  pilT Acinetobacter baylyi ADP1

75.362

99.424

0.749
  pilT Legionella pneumophila strain Lp02

73.451

97.695

0.718
  pilT Legionella pneumophila strain ERS1305867

73.451

97.695

0.718
  pilT Neisseria gonorrhoeae MS11

68.406

99.424

0.68
  pilT Neisseria meningitidis 8013

68.406

99.424

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.862

98.271

0.657
  pilT Vibrio cholerae strain A1552

66.862

98.271

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

99.424

0.499
  pilU Vibrio cholerae strain A1552

43.881

96.542

0.424
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.542

0.401