Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   G7047_RS20375 Genome accession   NZ_CP049910
Coordinates   4448605..4449741 (+) Length   378 a.a.
NCBI ID   WP_166309537.1    Uniprot ID   A0A6G8CT80
Organism   Diaphorobacter sp. HDW4A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4443605..4454741
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G7047_RS20345 (G7047_20350) - 4443880..4444296 (-) 417 WP_166309525.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  G7047_RS20350 (G7047_20355) - 4444293..4444634 (-) 342 WP_166309527.1 hypothetical protein -
  G7047_RS20355 (G7047_20360) - 4444647..4445783 (-) 1137 WP_166309529.1 aminotransferase class V-fold PLP-dependent enzyme -
  G7047_RS20360 (G7047_20365) - 4445923..4446645 (-) 723 WP_166309531.1 YggS family pyridoxal phosphate-dependent enzyme -
  G7047_RS20365 (G7047_20370) pilT 4446764..4447807 (+) 1044 WP_166309533.1 type IV pilus twitching motility protein PilT Machinery gene
  G7047_RS20370 (G7047_20375) - 4447873..4448505 (+) 633 WP_166309535.1 cyclic nucleotide-binding domain-containing protein -
  G7047_RS20375 (G7047_20380) pilU 4448605..4449741 (+) 1137 WP_166309537.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  G7047_RS20380 (G7047_20385) - 4449803..4450717 (+) 915 WP_166309539.1 NAD(P)-dependent oxidoreductase -
  G7047_RS20385 (G7047_20390) - 4450972..4451748 (-) 777 WP_166309541.1 BON domain-containing protein -
  G7047_RS20390 (G7047_20395) - 4451748..4452341 (-) 594 WP_166309543.1 SIS domain-containing protein -
  G7047_RS20395 (G7047_20400) - 4452441..4452806 (-) 366 WP_166309545.1 YraN family protein -
  G7047_RS20400 (G7047_20405) rsmI 4452869..4453804 (+) 936 WP_166309547.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  G7047_RS20405 (G7047_20410) - 4454135..4454539 (-) 405 WP_166309549.1 hypothetical protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41804.27 Da        Isoelectric Point: 6.9077

>NTDB_id=428572 G7047_RS20375 WP_166309537.1 4448605..4449741(+) (pilU) [Diaphorobacter sp. HDW4A]
MERDQASKFINDLLRLMVSRGGSDLFITAEFPPAIKVDGKITKVSPQPLTPSHTLMLARSIMSDKQVADFERTKECNFAI
SPAGIGRFRVNAFVQQGRVGLVLRTIPLTLPTIDGLGVPQVLKDVTMAKRGLCILVGATGSGKSTTLAAMLDWRNENSYG
HIITVEDPVEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRALVSQRLIPKQDGKGRAAAVEIMLNSPLIADLIFKGEVAEIKEIMKKSRNLG
MQTFDQALFDLFEANMITYEDALRNADSVNDLRLQIKLSSQRAKTTDLSAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=428572 G7047_RS20375 WP_166309537.1 4448605..4449741(+) (pilU) [Diaphorobacter sp. HDW4A]
ATGGAACGCGATCAGGCCAGTAAATTCATCAACGATCTGCTGCGCCTGATGGTCAGTCGGGGGGGGAGTGACCTGTTCAT
CACGGCCGAGTTTCCTCCCGCCATCAAGGTGGACGGCAAGATCACCAAGGTTTCGCCCCAGCCGCTGACCCCTTCGCACA
CCTTGATGCTCGCGCGCTCAATCATGAGCGACAAGCAGGTGGCGGATTTCGAGCGCACCAAGGAATGCAACTTCGCGATC
TCGCCTGCAGGCATCGGCCGCTTTCGCGTGAATGCCTTCGTGCAGCAGGGCCGCGTCGGGCTGGTGCTGCGGACGATTCC
GCTCACGCTGCCGACCATCGACGGTCTCGGCGTGCCGCAGGTACTCAAGGATGTGACCATGGCCAAGCGCGGCCTGTGCA
TTCTGGTGGGTGCGACCGGCTCGGGCAAATCGACCACACTGGCCGCCATGCTCGACTGGCGCAATGAAAACTCCTACGGT
CACATCATCACCGTGGAAGACCCGGTGGAATTCGTGCACCCGCACAAGAACTGCGTGGTCACCCAGCGTGAGGTGGGCCT
AGATACCGACAGCTGGGAAGCCGCGCTCAAGAACACGCTGCGCCAAGCACCCGACGTCATTCTGATGGGCGAAATCCGCG
ACCGCGAGACCATGGAACACGCCGTGGCCTTCGCCGAAACCGGGCACCTGTGCCTTGCTACGCTGCACGCCAACAGTGCC
AACCAGGCGCTGGACCGCATCATCAACTTCTTCCCCGAAGAACGCCGAGCACAGCTGCTGATGGACCTGTCGCTGAACCT
GCGTGCCCTCGTCTCGCAGCGCCTGATCCCCAAGCAGGACGGCAAGGGCCGCGCCGCCGCCGTGGAGATCATGCTGAACT
CGCCGCTGATCGCCGACTTGATCTTCAAGGGCGAGGTCGCTGAGATCAAGGAAATCATGAAGAAGAGCCGCAACCTCGGC
ATGCAGACGTTTGACCAGGCGCTGTTCGACCTGTTCGAAGCCAACATGATCACGTATGAGGACGCCCTTCGAAACGCCGA
CTCGGTCAACGACCTGCGCCTGCAGATCAAGCTGAGCAGCCAGCGCGCCAAGACCACCGACCTGTCGGCCGGCACAGAGC
ACTTCGCAATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8CT80

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.47

92.328

0.595
  pilU Acinetobacter baylyi ADP1

57.263

94.709

0.542
  pilU Vibrio cholerae strain A1552

54.023

92.063

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.291

89.153

0.413
  pilT Pseudomonas aeruginosa PAK

45.401

89.153

0.405
  pilT Acinetobacter baumannii D1279779

45.045

88.095

0.397
  pilT Acinetobacter nosocomialis M2

45.045

88.095

0.397
  pilT Acinetobacter baumannii strain A118

45.045

88.095

0.397
  pilT Legionella pneumophila strain Lp02

44.144

88.095

0.389
  pilT Legionella pneumophila strain ERS1305867

44.144

88.095

0.389
  pilT Pseudomonas stutzeri DSM 10701

43.62

89.153

0.389
  pilT Acinetobacter baylyi ADP1

43.844

88.095

0.386