Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   G7048_RS08470 Genome accession   NZ_CP049905
Coordinates   1840759..1841802 (+) Length   347 a.a.
NCBI ID   WP_166067709.1    Uniprot ID   A0A6G8BV66
Organism   Diaphorobacter sp. HDW4B     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1835759..1846802
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G7048_RS08450 (G7048_08445) - 1836261..1837595 (-) 1335 WP_371747662.1 DUF445 domain-containing protein -
  G7048_RS08455 (G7048_08450) - 1837711..1838133 (-) 423 WP_166067706.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  G7048_RS08460 (G7048_08455) - 1838417..1839559 (-) 1143 WP_166067707.1 aminotransferase class V-fold PLP-dependent enzyme -
  G7048_RS08465 (G7048_08460) - 1839917..1840639 (-) 723 WP_166067708.1 YggS family pyridoxal phosphate-dependent enzyme -
  G7048_RS08470 (G7048_08465) pilT 1840759..1841802 (+) 1044 WP_166067709.1 type IV pilus twitching motility protein PilT Machinery gene
  G7048_RS08475 (G7048_08470) - 1841871..1842503 (+) 633 WP_166067710.1 cyclic nucleotide-binding domain-containing protein -
  G7048_RS08480 (G7048_08475) pilU 1842632..1843768 (+) 1137 WP_166067711.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  G7048_RS08485 (G7048_08480) - 1843826..1844743 (+) 918 WP_166067712.1 NAD(P)-dependent oxidoreductase -
  G7048_RS08490 (G7048_08485) - 1844820..1845614 (-) 795 WP_166067713.1 BON domain-containing protein -
  G7048_RS08495 (G7048_08490) - 1845614..1846207 (-) 594 WP_166067714.1 SIS domain-containing protein -
  G7048_RS08500 (G7048_08495) - 1846331..1846744 (-) 414 WP_166070876.1 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38143.95 Da        Isoelectric Point: 6.7745

>NTDB_id=428543 G7048_RS08470 WP_166067709.1 1840759..1841802(+) (pilT) [Diaphorobacter sp. HDW4B]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKTVHSMVYDIMSDAQRKAYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPKIFGELALKPRGLVLVTGPTGSGKSTTLAAMVNYLNETEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSNGVGMQTLDQN
LTDLVRRNIISPAEARAKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=428543 G7048_RS08470 WP_166067709.1 1840759..1841802(+) (pilT) [Diaphorobacter sp. HDW4B]
GTGGACATTACCCAACTGCTTGCATTCAGCGTCAAGAACAAAGCATCCGACTTGCACCTCTCCGCCGGCCTGCCGCCGAT
GATCCGGGTGCACGGCGATGTGCGCCGCATCAACGTCGACGCGCTCGATCACAAGACCGTGCACAGCATGGTCTACGACA
TCATGAGCGACGCGCAGCGCAAGGCCTACGAAGAATTCCTCGAAGTCGACTTTTCCTTCGAAATCGAAGGGCTGGCGCGT
TTCCGGGTGAACGCGTTCAACCAGAACCGGGGTGCGGCGGCGGTGTTCCGTACGATTCCAAGCAAGATCCTGACGCTGGA
ACAACTGAACGCGCCCAAGATCTTCGGCGAACTGGCACTCAAGCCGCGCGGTCTGGTGCTGGTGACCGGCCCTACCGGCT
CGGGCAAATCGACGACGCTGGCCGCGATGGTCAACTACCTGAACGAAACCGAGTACGGCCACATCCTCACCGTGGAAGAC
CCGATCGAATTCGTGCACGAATCCAAGAAGTGCCTGATCAACCAGCGCGAAGTCGGCCCGATGACGCTGTCGTTCGCCGC
CGCGCTGAAATCCGCACTGCGTGAAGATCCGGACGCGATTCTGGTGGGCGAAATGCGTGACTTGGAAACCATTCGCCTTG
CGATGACCGCCGCCGAAACCGGCCACTTGGTGTTCGGCACGCTGCACACCTCGAGCGCGGCCAAGACCATCGACCGTATC
ATCGACGTGTTCCCCGCCGAAGAAAAGGAAATGGTCCGCGCGATGCTCTCCGAATCGCTGCAGGCGGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGATGGACAAGGCCGTGTCGCGGCGCACGAAATCATGCTCGGCACCAGCGCGATCCGCAACCTGA
TCCGCGAAGCCAAGGTCGCGCAGATGTACTCCACGATCCAGACCAGCAACGGTGTGGGCATGCAGACGCTGGACCAGAAT
TTGACAGACTTGGTGCGCCGCAACATCATCAGCCCAGCCGAGGCGCGGGCCAAGGCCAAGATTCCGGAGAATTTCCCCGG
ATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8BV66

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.232

99.424

0.758
  pilT Acinetobacter nosocomialis M2

76.232

99.424

0.758
  pilT Acinetobacter baumannii D1279779

76.232

99.424

0.758
  pilT Acinetobacter baumannii strain A118

76.232

99.424

0.758
  pilT Pseudomonas stutzeri DSM 10701

75.652

99.424

0.752
  pilT Acinetobacter baylyi ADP1

75.362

99.424

0.749
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Neisseria gonorrhoeae MS11

68.116

99.424

0.677
  pilT Neisseria meningitidis 8013

68.116

99.424

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.449

98.271

0.663
  pilT Vibrio cholerae strain A1552

67.449

98.271

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

99.424

0.499
  pilU Vibrio cholerae strain A1552

44.179

96.542

0.427
  pilU Acinetobacter baylyi ADP1

41.908

99.712

0.418
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.542

0.403