Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   G7048_RS08480 Genome accession   NZ_CP049905
Coordinates   1842632..1843768 (+) Length   378 a.a.
NCBI ID   WP_166067711.1    Uniprot ID   A0A6G8BUY6
Organism   Diaphorobacter sp. HDW4B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1837632..1848768
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G7048_RS08455 (G7048_08450) - 1837711..1838133 (-) 423 WP_166067706.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  G7048_RS08460 (G7048_08455) - 1838417..1839559 (-) 1143 WP_166067707.1 aminotransferase class V-fold PLP-dependent enzyme -
  G7048_RS08465 (G7048_08460) - 1839917..1840639 (-) 723 WP_166067708.1 YggS family pyridoxal phosphate-dependent enzyme -
  G7048_RS08470 (G7048_08465) pilT 1840759..1841802 (+) 1044 WP_166067709.1 type IV pilus twitching motility protein PilT Machinery gene
  G7048_RS08475 (G7048_08470) - 1841871..1842503 (+) 633 WP_166067710.1 cyclic nucleotide-binding domain-containing protein -
  G7048_RS08480 (G7048_08475) pilU 1842632..1843768 (+) 1137 WP_166067711.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  G7048_RS08485 (G7048_08480) - 1843826..1844743 (+) 918 WP_166067712.1 NAD(P)-dependent oxidoreductase -
  G7048_RS08490 (G7048_08485) - 1844820..1845614 (-) 795 WP_166067713.1 BON domain-containing protein -
  G7048_RS08495 (G7048_08490) - 1845614..1846207 (-) 594 WP_166067714.1 SIS domain-containing protein -
  G7048_RS08500 (G7048_08495) - 1846331..1846744 (-) 414 WP_166070876.1 YraN family protein -
  G7048_RS08505 (G7048_08500) rsmI 1846762..1847712 (+) 951 WP_166067715.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  G7048_RS08510 (G7048_08505) - 1848036..1848434 (-) 399 WP_166067716.1 hypothetical protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41818.24 Da        Isoelectric Point: 6.9077

>NTDB_id=428544 G7048_RS08480 WP_166067711.1 1842632..1843768(+) (pilU) [Diaphorobacter sp. HDW4B]
MERDQASKFINDLLRLMVSRGGSDLFITAEFPPAIKVDGKITKVSPQPLTPAHTLILARSIMSDKQIADFERSKECNFAI
SPAGIGRFRVNAFVQQGRVGLVLRTIPLTLPTIDGLGVPQVLKDVTMTKRGLCILVGATGSGKSTTLAAMLDWRNENSYG
HIITVEDPVEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIINFFPEERRGQLLMDLSLNLRAIVSQRLIPKQDGKGRSAAVEIMLNSPLIADLIFKGEVAEIKEIMKKSRNLG
MQTFDQALFDLFEANMITYEDALRNADSVNDLRLQIKLSSQRAKTTDLSAGTEHFSIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=428544 G7048_RS08480 WP_166067711.1 1842632..1843768(+) (pilU) [Diaphorobacter sp. HDW4B]
ATGGAACGCGATCAGGCCAGTAAATTCATCAACGATCTGCTTCGCCTGATGGTCAGCCGTGGAGGCAGTGACCTGTTCAT
CACCGCCGAATTTCCTCCCGCCATCAAGGTGGACGGCAAGATCACCAAGGTCTCGCCCCAGCCGCTGACGCCTGCGCACA
CGCTGATCCTCGCGCGCTCGATCATGAGCGACAAGCAGATCGCCGACTTCGAGCGCTCCAAGGAATGCAACTTCGCGATT
TCGCCAGCGGGCATTGGTCGCTTTCGTGTGAACGCCTTCGTGCAGCAGGGCCGCGTCGGTCTGGTGCTGCGGACGATTCC
GCTGACGCTACCGACCATCGATGGCCTTGGCGTGCCGCAGGTGCTCAAGGACGTGACCATGACCAAGCGCGGCCTGTGCA
TTCTGGTGGGCGCGACCGGCTCGGGCAAATCGACCACGCTGGCCGCCATGCTCGACTGGCGCAACGAGAATTCCTACGGC
CACATCATCACCGTGGAAGACCCGGTGGAATTCGTGCACCCGCACAAGAATTGCGTGGTCACGCAGCGTGAAGTGGGTCT
GGATACCGACAGCTGGGAAGCCGCGCTGAAGAACACGCTGCGTCAGGCTCCCGACGTGATTCTGATGGGCGAAATCCGCG
ACCGCGAAACCATGGAGCATGCGGTGGCATTCGCCGAAACCGGTCACCTGTGTCTGGCCACGCTGCACGCCAACTCGGCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTCCCCGAAGAACGTCGCGGGCAGTTGCTGATGGACTTGTCGCTGAACCT
GCGCGCCATCGTCTCGCAGCGCCTGATTCCCAAGCAGGACGGCAAGGGCCGCTCCGCCGCCGTGGAAATCATGCTGAACT
CGCCGCTGATTGCCGACCTGATCTTCAAGGGCGAGGTCGCCGAGATCAAGGAAATCATGAAGAAGAGCCGCAACCTCGGC
ATGCAGACCTTCGATCAGGCGCTGTTCGACCTGTTCGAGGCCAACATGATCACCTACGAAGACGCGCTCAGAAACGCCGA
CTCGGTCAACGACCTGCGCCTGCAGATCAAGCTGAGCAGCCAGCGCGCCAAGACCACCGACCTGTCGGCCGGTACCGAAC
ATTTCTCGATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8BUY6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.043

92.328

0.601
  pilU Acinetobacter baylyi ADP1

56.825

94.974

0.54
  pilU Vibrio cholerae strain A1552

53.736

92.063

0.495
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.697

89.153

0.407
  pilT Pseudomonas aeruginosa PAK

45.104

89.153

0.402
  pilT Acinetobacter baumannii strain A118

45.045

88.095

0.397
  pilT Acinetobacter baumannii D1279779

45.045

88.095

0.397
  pilT Acinetobacter nosocomialis M2

45.045

88.095

0.397
  pilT Legionella pneumophila strain Lp02

44.144

88.095

0.389
  pilT Legionella pneumophila strain ERS1305867

44.144

88.095

0.389
  pilT Pseudomonas stutzeri DSM 10701

43.62

89.153

0.389
  pilT Acinetobacter baylyi ADP1

43.544

88.095

0.384